LIVER HISTOPATHOLOGY AT MICE WITH INDUCED DIABETES TREATED WITH VEGETAL EXTRACT OF SYLIBUM MARIANUM

In this study we have followed the liver’s histopathologic changes at CD-line mice (mice rederived from a nonconsanguineous stem) with alloxan-induced diabetes, treated with vegetal extract of Sylibum marianum. We have followed the liver’s changes of weight following the removal and its histopathologic changes. Thus, mice with weight ranging from 20 to 25 g were divided into three experimental lots, a witness lot, a lot of mice with alloxan-induced diabetes, and another lot with diabetes treated with vegetal extract of Sylibum marianum. Diabetes was induced by administering two doses of Alloxan monohydrate 0,2 ml (130 mg/kg body dissolved in physiological saline), following which 0.2 ml of vegetal extract of milk thistle was administered by gavage during two weeks. The results obtained for the liver blades sampled from the mice with diabetes showed the presence of hepatic steatosis; following the administration of the vegetal extract to the mice with diabetes a decrease of the blisters full of neutral lipids (hepatic triglycerides) can be noticed, which reveals the beneficial action of the vegetal extract of milk thistl

INVESTIGATION ON THE HYPOGLYCEMIANT EFFECT OF SOME VEGETAL EXTRACTS IN EXPERIMENTAL INTOXICATIONS AT CD-1 MICE

Using plants in treating some diseases has become a tradition, ‘the nature’s pharmacy’ being an important source of therapy. At present, the medicinal plants properties are being re-assessed due to progress made in chemical, pharmaceutical and clinical research of plants and due to forms obtained from vegetal products, but especially due to the advantages they offer. In the context of more frequent use of natural products with pharmaceutical and therapeutical aims, both at a national and at an international level, our research hints at highlighting and giving details about a series of effects produced by vegetal extracts of Aronia melanocarpa and Silybum marianum on certain physiological, biochemical and histopathological processes at CD1 mice. In this study, 2 hydro-alcoholic extracts obtained from 2 species of medicinal plants (Aronia melanocarpa and Silybum marianum), were tested in order to analyse the hypoglycemiant activity. The data accumulated in the specialty literature reveal that the phytotherapeutic use of extracts of Aronia melanocarpa and Silybum marianum is based only on the major pharmacologic effect while the intimate action mechanism of the two vegetal products, al cellular and subcellular level, is not known. Starting from these premises, we considered useful the initiation of a comparative study regarding the antidiabetic influence of fluid extracts of Aronia melanocarpa and Silybum marianum upon mice experimentally intoxicated with alloxan monohydrate solution. CDI mice were used as an experimental model in order to induce diabetes: alloxan monohydrate was injected intraperitoneal with concentration of 130 mg/kg body (Ahmed Saber Abu – zaiton, 2013), dissolved in physiological serum, during two weeks, at an interval of 3 days. Both extracts acted positively by lowering blood sugar and by returning to normal body weight in diabetic mice. Aronia extract has a pronounced effect compared to milk thistle extract for both parameters monitored in the experiments

STUDY ON THE HEPATOPROTECTIVE EFFECT OF SILYBUM MARIANUM EXTRACT ON EXPERIMENTAL POISONING BY PARACETAMOL IN NMRI ALBINO MICE

Hepatoprotection is a matter of worldwide interest, since liver diseases are common, and liver transplant has increasedover the past years, drastically reducing the number of people able to meet the criteria for such a transplant. The experimental research in this paper aimed at evaluating the hepatoprotective capacity of Silybum marianum species (thistle). In order to fulfil the purpose of the paper, thistle was administrated as hydr alcoholic extracts to animals, i.e. albino mice-NMRI strain. We used paracetamol solution for infusion known as Perfalgan (Bristol-Myers Squibb). The dose used was 400mg/kg/bod y substance, and we administrated ethanolic extracts of Sylibum marianum after the paracetamol poisoning. We conducted the research using biochemical methods and techniques, potential structural and functional changes occurred in the experimental animals’ internal organs poisoned with perfalgan then treated with Silybum marianum plant extract

STUDY ON THE HEPATOPROTECTIVE EFFECT OF PLANTAGO MAJOR EXTRACT ON EXPERIMENTAL POISONING BY DICLOFENAC IN NMRI ALBINO MICE

Medicinal plants are alternative medicine’s key weapons. Thanks to the medicinal effects some plants turned out to have, they have been chosen by people to heal or at least ameliorate various affections. Nature is continuously changing: some species become extinct, others are born and some cross time. Despite the trend to enhance plant-based medicines, research data in this field is still incomplete. The experimental research in this paper aimed at evaluating the hepatoprotective capacity of the Plantago major (plantain) species in albino mice –NMRI stain, after experimental poisoning by Diclofenac. Using physiological and biochemical methods and techniques, we researched potential structural and functional changes ocurred further to the experimental poisoning by Diclofenac

EVOLUTION OF THE HAEMATOLOGICAL INDICES AT CD1 MICE WITH INDUCED DIABETES, TREATED WITH VEGETAL EXTRACTS OF ARONIA MELANOCARPA AND SILYBUM MARIANUM

The aim of this paper is to investigate the effects produced by the vegetal extracts of Aronia melanocarpa and Silybum marianum on the haematological index at CD1 (the rederived mice from a non-consanguineous strain) mice with artificially induced diabetes. We established the number of haematites and leucocytes, as well as their morphological dimension and aspect on the blood smear. The diabetisation of the mice was carried out by administering two doses of 0.2 ml solution of Alloxan (130mg/kg body, dissolved in physiological saline), following which, during two weeks, vegetal extract of aronia and milk thistle was administered (0,2 ml). The results obtained showed that there are no significant changes of the number of haematites and leucocytes in comparison with the witness lot. As for the aspect and the dimensions of the figurate elements on the blood smear, in the case of the lot injected with alloxan monohydrate, it was noticed an increase of the haematites’ volume and the apparition of some atypical cells – megalocytes, as well as the presence of apoptosis nuclei. Following the administration of the vegetal extracts of aronia and milk thistle, we notice a comeback of the haematites’ dimension, as well as the lack of apoptosis micronuclei, which demonstrates the beneficial effect of the two extracts