Can biofortified plants accumulate trace elements essential to the growth and development of humans?

Magister Scientiae (Biodiversity and Conservation Biology)This study aimed to determine the nutrient content (Co, Cr, F, I, Se and V) of various vegetable based food items collected from the Cape Town area in the Western Cape Province of South Africa. This was done to determine which vegetable crops provided the highest concentrations of essential trace elements, and how much they contribute to the daily recommended intake (DRIs) of these trace elements. It also aimed to assess the effects of the addition of the trace elements (Co, Cr, F, I, Se, Si, Sn and V) on seed germination and root growth under controlled conditions in order to calculate their phytotoxicity, and then to biofortify four vegetable crop species, grown in sand culture, with a composite treatment of the trace elements to determine how the addition of these elements will affect the vegetable crops grown under these experimental conditions. From this study, it was shown that trace element content in vegetable crops in the Western Cape Province of South Africa varied between different geographic locations and that certain trace elements were absent from several items collected from some areas. Although some crop species contained sufficient amounts of certain trace elements to satisfy our daily recommended intakes, most of the crops were found to contain insufficient amounts of many of the trace elements to satisfy our needs. Leafy vegetables and tubers were identified as the better vegetable types to biofortify with essential trace elements as they already contain higher concentrations of several of the essential trace elements and should thus be assessed for their effectiveness as crops to be biofortified. When the trace elements were applied directly to cress and lettuce seeds, it was found that all the trace elements, as well as the composite treatments, exerted phytotoxic effects on cress and/or lettuce seeds when applied athighconcentrations. Lettuce was found to be more prone to the effects of these elements. Seed germination was strongly inhibited by fluoride, while several elements affected root growth. When fluoride was left out of the composite treatment, phytotoxicity only occurred at high concentrations. The addition of the trace elements at the high concentrations to already established spinach, cabbage, lettuce and turnip plants were found to affect the uptake of several essential plant nutrients, but the concentrations of the elements affected generally remained higher than the concentrations needed for adequate growth of agricultural crops. Several of the trace elements supplied to the plants were also found to be retained in the roots of the vegetable crops however, as the concentrations supplied to the plants increased, so did the concentrations found in the edible portions of the crop

Native legume species from the Northern Cape province of South Africa and their potential use as forage crops.

Philosophiae Doctor - PhDThe lack of bioclimatically suitable forage species for livestock production in the water-limited agro-ecological areas of South Africa has resulted in significant feed shortages within these areas during the ongoing drought experienced within the country. This, in turn, has resulted in significant livestock mortalities leading to financial difficulties for the farmers and farming communities within these areas. Thus, many of the water-limited agro-ecological areas in South Africa have been declared disaster areas. These cyclic long-term droughts, as well as more common short-term droughts are expected to increase in frequency, duration and intensity under the predicted future bioclimatic conditions. Although there has been significant investment into the development of improved, better-adapted forage crops for these bioclimatically marginal agro-ecosystems, these efforts, to date, have largely been unsuccessful. Therefore, in this study, we propose to identify and evaluate species that are native to the water-limited South African agro-ecosystems and that can potentially be implemented in alternative fodder flow programs within these water-limited agro-ecological areas

Seed germination and early seedling growth responses to drought stress in annual Medicago L. And Trifolium L. Forages

Climate change in the Mediterranean-like regions of South Africa has resulted in increased rainfall variability, a delayed start to the wet winter season, and increased occurrences of out-ofseason summer rainfall events. These changes in bioclimatic conditions are predicted to become more pronounced and, therefore, could result in poor seedling establishment and false breaks from the soil seed bank, followed by seedling desiccation in annual medic and clover pastures. This study therefore aimed to quantify and compare the responses of three annual Medicago species and five annual Trifolium species to drought stress imposed at the seed germination, seedling establishment, and early seedling growth stages. Three separate controlled trials were conducted. Firstly, the seeds were germinated in seed germination chambers at constant temperatures ranging from 5 to 30 ◦C, in 5 ◦C increments, with five osmotic treatments within each temperature, and germination was recorded daily. For the second experiment, seeds were planted at 100, 70, 50, and 30% of the soil’s moisture-holding capacity without subsequent watering, and emergence was recorded daily. For the third experiment, one-month-old seedlings were subjected to two water-limitation periods (15 or 30 days), and their subsequent morphological responses were measured

Morphological and physiological responses of Calobota sericea plants subjected to water limitation and subsequent rewatering

Calobota sericea is a native legume of South Africa, confined to the water-limited rangelands, and it has recently been prioritised for additional characterisation regarding its pasture potential. In this study, we examined the growth characteristics of C. sericea under glasshouse conditions where water limitation was implemented at different plant ages, and for different durations. Results indicate that preferential resource allocation to the roots, as well as reduced stomatal conductance and transpiration, were early responses to water limitation, irrespective of the age at which water limitation was imposed, or the duration of water limitation. Under water-limited conditions, increased production of protective pigments, such as carotenoids and anthocyanins, was also observed, which helped in recovery after rewatering. It was concluded that after rewatering, all negative impacts of water limitation on morphology and physiology of C. sericea plants were generally returned to well-watered levels. This suggests that C. sericea plants employ a wide range of phenotypic adjustments in response to water limitation, which makes the plants well adapted to areas with high rainfall variability

Nutritional quality of Calobota sericea fodders. A preliminary assessment

This study aimed to provide preliminary information regarding the nutritional quality of Calobota sericea, a preferred perennial legume forage species from the water-limited rangelands of South Africa. Calobota sericea plant samples were collected from the Namaqualand rangelands in the wet and dry season and analysed for secondary compounds, fibre, protein and mineral nutrient content. The results from the fibre analyses were used to determine the digestibility and energy content of fodders. Preliminary results indicate that C. sericea fodders are of better nutritional quality in the wet season and that protein content, digestibility and energy content is sufficient for maintenance of lambs and dry ewes. The energy content, however, was not sufficient for maintenance of pregnant and lactating ewes. Furthermore, certain mineral nutrients (Na, P and K) were not found in sufficient concentrations in this species, and it was thus suggested that further investigation is needed into whether fertilisation could potentially improve the protein, digestibility and mineral nutrient content of C. sericea fodders

Nutritional quality of Calobota sericea fodders: A preliminary assessment

This study aimed to provide preliminary information regarding the nutritional quality of Calobota sericea, a preferred perennial legume forage species from the water-limited rangelands of South Africa. Calobota sericea plant samples were collected from the Namaqualand rangelands in the wet and dry season and analysed for secondary compounds, fibre, protein and mineral nutrient content. The results from the fibre analyses were used to determine the digestibility and energy content of fodders. Preliminary results indicate that C. sericea fodders are of better nutritional quality in the wet season and that protein content, digestibility and energy content is sufficient for maintenance of lambs and dry ewes. The energy content, however, was not sufficient for maintenance of pregnant and lactating ewes. Furthermore, certain mineral nutrients (Na, P and K) were not found in sufficient concentrations in this species, and it was thus suggested that further investigation is needed into whether fertilisation could potentially improve the protein, digestibility and mineral nutrient content of C. sericea fodders

The Potential for Endozoochorous Dispersal of <i>Vachellia nilotica</i> Seeds by Goats: Implications for Bush Encroachment

Seed dispersal has become an important component in understanding plant–animal interaction. Recently, there have been concerns about the role of ruminants, particularly browsers, in the dispersal of woody plant seeds. This study aimed to determine whether including Vachellia nilotica seeds in livestock, diets could reduce the spread of viable seeds in the rangelands and control bush encroachment. The shoots and seed pods of Vachellia nilotica were harvested and analyzed for fiber, protein, and mineral nutrients at different seed pods inclusion rates, with or without a feed additive. Six diets were selected for feeding 24 goats and quantifying seed recovery and germination after ingestion. Results indicated that including seed pods and feed additives to Vachellia nilotica shoots significantly improved the quality of the fodder. Chipping the seed pods prior to including them in the diet resulted in 13% intact seed recovery, and approximately 2% of these seeds were recovered after ingestion. These recovered seeds were mostly still viable but were still dormant as seed coats were not sufficiently damaged after ingestion. Therefore, viable seeds may still be dispersed in the rangeland, leading to further bush encroachment

Seed Germination and Early Seedling Growth Responses to Drought Stress in Annual <i>Medicago</i> L. and <i>Trifolium</i> L. Forages

Climate change in the Mediterranean-like regions of South Africa has resulted in increased rainfall variability, a delayed start to the wet winter season, and increased occurrences of out-of-season summer rainfall events. These changes in bioclimatic conditions are predicted to become more pronounced and, therefore, could result in poor seedling establishment and false breaks from the soil seed bank, followed by seedling desiccation in annual medic and clover pastures. This study therefore aimed to quantify and compare the responses of three annual Medicago species and five annual Trifolium species to drought stress imposed at the seed germination, seedling establishment, and early seedling growth stages. Three separate controlled trials were conducted. Firstly, the seeds were germinated in seed germination chambers at constant temperatures ranging from 5 to 30 °C, in 5 °C increments, with five osmotic treatments within each temperature, and germination was recorded daily. For the second experiment, seeds were planted at 100, 70, 50, and 30% of the soil’s moisture-holding capacity without subsequent watering, and emergence was recorded daily. For the third experiment, one-month-old seedlings were subjected to two water-limitation periods (15 or 30 days), and their subsequent morphological responses were measured. The results from these experiments indicated that the species differed significantly in their responses to drought, and the best-performing species often differed when drought was imposed at different development stages. Five species—M. polymorpha L., M. truncatula Gaertn., T. alexandrinum L., T. vesiculosum Savi., and T. subterraneum L. ssp. subterraneum—were able to tolerate incidences of drought better than other species and, thus, should be prioritized for further research into the variation in drought tolerance between cultivars within these species

The impacts of season and livestock management strategy on the quality of diets selected by goats and sheep in the semi-arid rangelands of Namaqualand, South Africa

Access to good-quality forages is one of the major limitations to livestock production in semi-arid pastoral systems. This study aimed to determine whether there are differences in the nutritional quality of diets selected by herded and free-ranging goat and sheep flocks utilising Namaqualand Granite Renosterveld vegetation during the wet and dry seasons. Plant samples collected along the grazing routes of livestock were dried and analysed for their fibre, condensed tannin, total phenolic and mineral nutrient contents. The study showed that a large variety of forages were on offer and livestock groups selected different diets of which some were different to the total diet on offer. In general, significant deficiencies in phosphate, protein and energy in the diets selected by herded and free-ranging goats and sheep were observed in both wet and dry season. The quality of the diets selected by herded and free-ranging livestock was also found to be different from each other, with herded livestock generally selecting more nutrient-dense diets. Herding, therefore, allows livestock to access better-quality forage in the Namaqualand Granite Renosterveld rangeland, where it is generally poor.Keywords: herding, Namaqualand Granite Renosterveld, pastoral livestock, protein deficiencies, small stock nutritio