Molecular cytogenetic characterisation of Salix viminalis L. using repetitive DNA sequences

Abstract Salix viminalis L. (2n=38) is a diploid dicot species belonging to the Salix genus of the Salicaceae family. This short-rotation woody crop is one of the most important renewable bioenergy resources worldwide. In breeding for high biomass productivity, limited knowledge is available on the molecular cytogenetics of willow, which could be combined with genetic linkage mapping. The present paper describes the adaptation of a fluorescence in situ hybridisation (FISH) protocol as a new approach to analyse the genomic constitution of Salix viminalis using the heterologous DNA clones pSc119.2, pTa71, pTa794, pAs1, Afafamily, pAl1, HT100.3, ZCF1 and the GAA microsatellite marker. Three of the nine probes showed unambiguous signals on the metaphase chromosomes. FISH analysis with the pTa71 probe detected one major 18S-5.8S-26S rDNA locus on the short arm of one chromosome pair; however, the pTa794 rDNA site was not visible. One chromosome pair showed a distinct signal around the centromeric region after FISH with the telomere-specific DNA clone HT100.3. Two chromosome pairs were found to have pAs1 FISH signals, which represent a D-genome-specific insert from Aegilops tauschii. Based on the FISH study, a set of chromosomes with characteristic patterns is presented, which could be used to establish the karyotype of willow species

Az átmenetifémionok peptidekkel alkotott komplexei. A fémion-fehérje kölcsönhatás modellezése. = Transition metal complexes of peptides. Models of the metal ion protein interactions.

1. Multihisztidin peptidek réz(II)- és cink(II)komplexei: A hisztidin nitrogén donoratomok a peptidek elsődleges fémkötőhelyei. Ezek koordinációjával makrokelátok képződhetnek, amelyek stabilitása a hisztidinek számától és távolságától függ. A karboxilcsoportok jelenléte a cink(II)komplexek stabilitását növeli. A réz(II)ionok az amidcsoport deprotonálódását is indukálhatják, ami többmagvú komplexek képződéséhez vezet. A megkötött rézionok száma megegyezik a hisztidinek számával. 2. A prion protein peptid fragmenseinek fémkomplexei: Az oktarepeaten kívüli hisztidinek is stabilis rézkötőhelyek. A HuPrP(84-114) fragmensre kapott eredmények szerint a kötési helyek stabilitási sora: His111 > His96 >> His85. Egyéb átmenetifémek komplexeit is tanulmányoztuk, amelyek stabilitási sora a következő: Pd(II) > Cu(II) > Ni(II) > Zn(II) > Cd(II) ~ Co(II) > Mn(II). 3. Az amyloid-? peptid réz(II)komplexei: Az A?(1-16) peptidnek kiugróan nagy rézionaffinitása van. A terminális aminocsoport az elsődleges fémkötőhely, amit a hisztidinek koordinációja követ. Egy A?(1-16) molekula 4 réziont képes megkötni. Az egy- két- és három-magvú komplexeknek koordinációs izomerjei lehetnek, de a terminális aminocsoport és a szomszédos amidnitrogének koordinációja preferált. | 1. Copper(II) and zinc(II) complexes of multihistidine peptides: Histidyl residues are the primary metal binding sites resulting in the formation of macrochelates. The stabilities of macrochelates are influenced by the number and location of histidyl residues. The stability of zinc(II) complexes is enhanced by the presence of carboxylate functions. Formation of polynuclear complexes has also been detected and their nuclearities correspond to the number of histidyl sites. 2. Metal binding affinity of prion peptide fragments: Histidyl residues outside the octarerepat domain are effective copper binding sites. The results obtained for the copper(II) complexes of HuPrP(84-114) revealed the following stability order: His111 > His96 >> His85. Complex formation with several other transition elements has also been studied and their stability order: Pd(II) > Cu(II) > Ni(II) > Zn(II) > Cd(II) ~ Co(II) > Mn(II). 3. Copper(II) complexes of amyloid-? peptide fragments: A?(1-16) has an outstanding affinity towards the complexation with copper. The terminal amino group is the primary metal binding site, followed by the coordination of histidyl residues. One molecule of A?(1-16) can bind as much as four copper(II) ions. Various coordination isomers of the mono-, di- and tri-nuclear complexes can exist with a preference for the coordination via the terminal amino and subsequent amide groups

Quality Education and Digitalization Efforts, the Catalysts of Sustainable Development

Education, science, technology, research and innovation are the supporting tools of the European Union's Sustainable Development 2030 program. They are valuable elements in themselves, the priority of which cannot be doubted, since they act as catalysts. What should be the direction to follow with the help of these factors in order to preserve competitiveness within the framework of sustainability? The solution is a factor affecting the lives of all of us. The world of business, education and this resent are trying to find a solution to this challenge. Sustainable development is a priority goal of the internal and external policies of the European Union, among the 17 Sustainable Development Goals (SDGs), the member states undertake to implement quality education and develop digital competence. The Erasmus+ education and training EU (European Union) programs help to overcome the differences between Member States and regions

The first reported case of trichothiodystrophy in Hungary: A young male patient with mutations in the ERCC2 gene

Trichothiodystrophy, also called sulphur-deficient brittle hair syndrome, is a rare autosomal recessive genetic disorder of DNA repair and transcription. Trichothiodysthrophy is characterised by dry, thin, easily broken hair, showing alternating light and dark pattern called ‘tiger tail’ banding under polarizing light microscopy. According to our knowledge, our report is the first one on this rare disorder from Hungary: a case of a 9-year-old boy showing clinical features typical of trichotiodystrophy. Sequence analysis of the ERCC2 gene identified two recurrent trichothidodystrophy missense heterozygous mutations – c.934G/A p.Asp312Asn (CM015299) and c.2251A/C p.Lys751Gln (CM004814) – suggesting compound heterozygous state of the patient and confirming the clinically suspected diagnosis of trichothiodystrophy.  </p

Országos Táplálkozás és Tápláltsági Állapot Vizsgálat 2014. I. A magyar felnőtt lakosság tápláltsági állapota | Hungarian Diet and Nutritional Status Survey 2014 I. Nutritional status of the Hungarian adult population

Absztrakt: Bevezetés és célkitűzés: Az Országos Táplálkozás és Tápláltsági Állapot Vizsgálat ötévenként, országos reprezentatív mintán határozza meg a felnőtt lakosság tápláltsági állapotát és táplálkozási szokásait, kiegészítve 2014 óta a fizikai aktivitás mérésével. Módszer: A felmérés azonos típusú eszközökkel végzett antropometriai mérésekkel, nemzetközi protokoll alapján valósul meg. Eredmények: A jelen vizsgálattal megegyező módszertannal zajló első adatgyűjtés 2009-ben, az Európai Lakossági Egészségfelméréshez kapcsolódva történt. A 2014-es eredmények szerint a felnőttek csaknem kétharmada túlsúlyos vagy elhízott. A férfiak 28,2%-a, a nők 31,5%-a elhízott. A morbid elhízás aránya férfiak esetén 2,6%, nők esetén 3,3%. A hasi elhízás férfiaknál ritkább, mint nőknél (38% vs. 55%), előfordulása az életkorral nő. Az idősek körében a hasi elhízás a férfiak több mint 55%-át, a nők közel 80%-át érinti. Magyarországon a túlsúly, az elhízás és a hasi elhízás előfordulása igen magas. Következtetés: Az elhízás visszaszorítása érdekében célzott beavatkozásokra, illetve az intervenciók hatékonyságának monitorozására van szükség, amelyekhez a méréseken alapuló adatok elengedhetetlenek. Orv. Hetil., 2017, 158(14), 533–540. | Abstract: Introduction and aim: The Hungarian Diet and Nutritional Status Survey examines the obesity prevalence, dietary habits and, since 2014, physical activity in Hungarian adults in every 5 years. Method: The survey provides national data representative by age and gender, based on anthropometric measurements and international standards. Results: In 2014, nearly two-thirds of adults were overweight or obese. 28.2% of men and 31.5% of women were obese. Prevalence of morbid obesity were 2.6% and 3.3% in men and women, respectively. Abdominal obesity was more prevalent among women than men (55% vs. 38%), and the rate was increasing with age in both genders. In elderly, 55% of men and almost 80% of women were abdominally obese. Conclusion: In conclusion, the prevalence of overweight, obesity, and abdominal obesity is high in Hungarian adults. In order to tackle obesity, we need to obtain representative and measured data, which form the basis of targeted interventions and the assessment of their impact. Orv. Hetil., 2017, 158(14), 533–540

The first reported case of trichothiodystrophy in Hungary: A young male patient with mutations in the ERCC2 gene

Trichothiodystrophy, also called sulphur-deficient brittle hair syndrome, is a rare autosomal recessive genetic disorder of DNA repair and transcription. Trichothiodysthrophy is characterised by dry, thin, easily broken hair, showing alternating light and dark pattern called ‘tiger tail’ banding under polarizing light microscopy. According to our knowledge, our report is the first one on this rare disorder from Hungary: a case of a 9-year-old boy showing clinical features typical of trichotiodystrophy. Sequence analysis of the ERCC2 gene identified two recurrent trichothidodystrophy missense heterozygous mutations – c.934G/A p.Asp312Asn (CM015299) and c.2251A/C p.Lys751Gln (CM004814) – suggesting compound heterozygous state of the patient and confirming the clinically suspected diagnosis of trichothiodystrophy.  </p

Strontium titanate photocatalysts : preparation, characterization and photocatalytic activity

In this study strontium titanate photocatalysts were prepared via a hydrothermal method. The effect of various synthesis parameters (synthesis time, pH) on the properties of the samples obtained was investigated. The photocatalysts were characterized by X-ray diffractometry, scanning electron microscopy and transmission electron microscopy. The photocatalytic activity of the samples was evaluated by the photocatalytic reduction of carbon dioxide. A direct correlation was observed between the efficiency and strontium carbonate content of the photocatalysts