Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding-5

<p><b>Copyright information:</b></p><p>Taken from "Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding"</p><p>http://www.molecularneurodegeneration.com/content/2/1/23</p><p>Molecular Neurodegeneration 2007;2():23-23.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2211485.</p><p></p>ture incubator. Levels of holoAPP were measured from cell lysates with anti-APP antibody 369. Levels of Munc13-1 wild type and Munc13-1 H567K mutant proteins were measured by anti-GFP antibody. Equal protein loading was verified by measuring the levels of actin protein in all cell lysates

Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding-6

<p><b>Copyright information:</b></p><p>Taken from "Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding"</p><p>http://www.molecularneurodegeneration.com/content/2/1/23</p><p>Molecular Neurodegeneration 2007;2():23-23.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2211485.</p><p></p>ualization of (a) Munc13-1and (c) Munc13-1mutant molecules (green). (b, d) APP was immunolabeled with rabbit polyclonal anti-APP-specific antibody 369 followed by rhodamine red conjugated secondary antibody (red)

Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding-4

<p><b>Copyright information:</b></p><p>Taken from "Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding"</p><p>http://www.molecularneurodegeneration.com/content/2/1/23</p><p>Molecular Neurodegeneration 2007;2():23-23.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2211485.</p><p></p>esence (+) of PDBu. (B) Quantification of 3 such experiments

Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding-1

<p><b>Copyright information:</b></p><p>Taken from "Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding"</p><p>http://www.molecularneurodegeneration.com/content/2/1/23</p><p>Molecular Neurodegeneration 2007;2():23-23.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2211485.</p><p></p>ualization of (a) Munc13-1and (c) Munc13-1mutant molecules (green). (b, d) APP was immunolabeled with rabbit polyclonal anti-APP-specific antibody 369 followed by rhodamine red conjugated secondary antibody (red)

Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding-2

<p><b>Copyright information:</b></p><p>Taken from "Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding"</p><p>http://www.molecularneurodegeneration.com/content/2/1/23</p><p>Molecular Neurodegeneration 2007;2():23-23.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2211485.</p><p></p>of Munc18 (lanes 8–11). (B) Basal and PMA-stimulated sAPPα release were determined in the absence (lanes 1 and 6) or presence of wildtype (lanes 2 and 7) or phospho-site mutant forms of Munc18 (lanes 3–5 and 8–10)