Early Oral Ovalbumin Exposure during Maternal Milk Feeding Prevents Spontaneous Allergic Sensitization in Allergy-Prone Rat Pups

There are conflicting data to support the practice of delaying the introduction of allergenic foods into the infant diet to prevent allergy development. This study investigated immune response development after early oral egg antigen (Ovalbumin; OVA) exposure in a rat pup model. Brown Norway (BN) rat pups were randomly allocated into groups: dam reared (DR), DR pups challenged daily (days 4–13) with oral OVA (DR + OVAc), DR pups challenged intermittently (on day 4, 10, 12, and 13) with oral OVA (DR + OVAi), formula-fed pups (FF), and FF pups challenged daily with oral OVA (FF + OVA). Immune parameters assessed included OVA-specific serum IgE, IgG1, and IgA. Ileal and splenic messenger ribonucleic acid (mRNA) expression of transforming growth factor-beta (TGF-β1), mothers against decapentaplegic (Smad) 2/4/7, and forkhead box P3 (Foxp3) were determined. Ileum was stained for TGF-β1 and Smad4. Results. Feeding OVA daily to DR pups maintained systemic and local gut antibody and immunoregulatory marker mRNA responses. Systemic TGF-β1 was lower in DR + OVAi pups compared to DR and DR + OVAc pups. Feeding OVA to FF pups resulted in significantly greater OVA-specific IgE and IgG1, and lower IgA and TGF-β1 and Smad expression compared to DR pups. Conclusions. Early daily OVA exposure in the presence of maternal milk maintains immune markers associated with a regulated immune response, preventing early allergic sensitization

Effects of analgesic agents on blood biochemistry values of female Dark Agouti rats 72 hr after 5-FU or saline injection.

<p>Data are expressed as median values with experimental group range in brackets. Shaded results are outside references range values given by Giknis and Clifford, 2008 (Charles River labs for inbred Wistars).</p

Effect of analgesic agents on non-pain specific behaviours.

<p>Data are expressed as median (interquartile range) of behavioural frequency over 10 minute observation period unless duration indicated.</p

Ethogram for in-cage behavioural analysis (adapted from Roughan and Flecknell, 2001 [21]).

<p>Ethogram for in-cage behavioural analysis (adapted from Roughan and Flecknell, 2001 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158851#pone.0158851.ref021" target="_blank">21</a>]).</p

Alsophila pustulosa Christ.

原著和名: ヒカゲヘゴ モリヘゴ ヒヨケヘゴ科名: ヘゴ科 = Cyatheaceae採集地: 鹿児島県 沖永良部島 大山 (大隅 沖永良部島 大山)採集日: 1971/2/10採集者: 萩庭丈壽整理番号: JH029531国立科学博物館整理番号: TNS-VS-97953

The effects of orally-administered emu oil in a rat model of chemotherapy-induced mucositis

DDW 2009, May 30 - June 4, 2009, McCormick Place, Chicago, IllinoisRuth J. Lindsay, Mark S. Geier, Roger Yazbeck, Kerry A. Lymn, Ross N. Butler and Gordon S. Howart

Effects of analgesic agents on cumulative bodyweight change, food and water intake and urine output in saline-injected rats and in 5-FU injected rats from days 7–9.

<p>Data are expressed as means ± SEM.</p