Detection and genotyping of human papillomavirus in a sample of the cervix of adolescents from the Northwest Sanitary District of the city of Goiânia, Goiás

Submitted by Luciana Ferreira ([email protected]) on 2019-01-07T12:07:44Z No. of bitstreams: 2 Dissertação - Lyana Elias Santos Daud - 2005.pdf: 1938845 bytes, checksum: cc200d8d43bef3065b4fd57275735f93 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2019-01-07T13:12:15Z (GMT) No. of bitstreams: 2 Dissertação - Lyana Elias Santos Daud - 2005.pdf: 1938845 bytes, checksum: cc200d8d43bef3065b4fd57275735f93 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-01-07T13:12:15Z (GMT). No. of bitstreams: 2 Dissertação - Lyana Elias Santos Daud - 2005.pdf: 1938845 bytes, checksum: cc200d8d43bef3065b4fd57275735f93 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2005-03-31OutroBackground: Human Papillomavirus (HPV) infection is a strong predictor for cervical cancer development. Some HPV types are considered at high-risk and associated with pre-malignant lesions and cervical cancer. Genital HPV infection is highly prevalent in sexually active young women. Actually PGMY09/11 primer system associated with Restriction Fragment Length Polymorphism (RFLP), hibridization or DNA sequencing is one of the most sensitive methods for detecting and typing HPV-DNA in genital samples. Objectives: To detect and identify HPV-DNA genotypes in genital specimens among sexually active female adolescents from Distrito Sanitário Noroeste, Goiânia. Methods: We performed a cross-sectional study among 432 sexually active female adolescents (15-19 years), randomly selected at Distrito Sanitário Noroeste, Goiânia, and served by the Family Health Program. HPV-DNA was detected by PCR assay, in endocervical samples, using the PGMY09/11 consensus primer set. The presence of amplifiable DNA was assessed with -globin primers (PCO3/PCO4). Amplicons generated with PGMY primers were typed with the line blot assay (PGMY-line blot) and RFLP. Results: HPV-DNA was detected in 121 samples yielding a 28% prevalence (95% CI 23.8- 32.5). All samples yielded a -globin amplimer, confirming the DNA adequacy. Thirty different HPV genotypes were identified, the high risk genotypes frequently found being: HPV-16 (6,7%); followed by HPV-51 (5,1%); HPV-31 (4,6%); HPV-52 (4,2%) and HPV-18 (3,5%). PGMY-line blot test identified 54 cases of infection caused by multiple genotypes. The genotypes 16, 51, 18, 53 and 52 were the most frequently associated with cervical co- infection with multiple HPV types. Conclusions: A high prevalence of HPV-DNA was found and a broad spectrum of HPV genotypes was identified, predominantly high risk HPV genotypes. A high frequency of multiple HPV infections was found in the studied population.Introdução: A infecção pelo papilomavirus humano (HPV) é um fator causal necessário para o desenvolvimento do câncer cervical. Dos vários tipos de HPV conhecidos alguns sãoconsiderados como de alto risco oncogênico pela sua associação com lesões cervicais pré- neoplásicas e câncer cervical. A infecção genital pelo HPV é altamente prevalente em mulheres jovens sexualmente ativas. Atualmente o sistema de primers de consenso PGMY09/11 associado à análise de polimorfismo dos fragmentos gerados por enzimas de restrição (RFLP), à hibridização ou ao sequenciamento é um dos métodos mais sensíveis para detecção e tipagem do DNA-HPV em amostras genitais. Objetivos: Detectar o DNA-HPV e identificar os genótipos virais em amostras endocervicais de adolescentes sexualmente ativas do distrito sanitário noroeste do município de Goiânia. Metodologia: Realizou-se um estudo transversal com 432 adolescentes residentes no distrito sanitário noroeste do município de Goiânia e atendidas pelo programa de saúde da família. A detecção do DNA-HPV foi realizada em amostras endocervicais através da PCR empregando os primers de consenso PGMY09/11. A presença de DNA amplificável foi avaliada com os primers da -globina (PCO3/PCO4). A identificação genotípica do HPV foi feita a partir do produto da PCR através de hibridização reversa line blot e RFLP. Resultados: O DNA-HPV foi detectado em 121 amostras, o que correspondeu a uma prevalência de 28% (IC95% 23,8-32,5). Todas as 432 amostras foram amplificadas com os primers da -globina (PCO3/PCO4), confirmando a adequação do DNA. Foram identificados 30 genótipos diferentes de HPV sendo os mais prevalentes os de alto risco oncogênico: HPV-16 (6,7%), HPV-51 (5,1%), HPV-31 (4,6%), HPV-52 (4,2%) e HPV-18 (3,5%). A hibridização line blot possibilitou a identificação de 54 casos de infecções múltiplas, nos quais os HPVs 16, 51, 18, 53 e 52 foram os mais frequentes. Conclusão: A prevalência do DNA-HPV foi elevada e identificou-se um amplo espectro de genótipos do HPV, predominando os de alto risco oncogênico. A frequência de infecções múltiplas foi alta na população estudada

Prevalence, genotype profile and risk factors for multiple human papillomavirus cervical infection in unimmunized female adolescents in Goiânia, Brazil: a community-based study

Submitted by Luciana Ferreira ([email protected]) on 2019-10-16T14:12:04Z No. of bitstreams: 2 Artigo - Rosane Ribeiro Figueiredo Alves - 2013.pdf: 484238 bytes, checksum: 9b42ea9ac32099a21c09b8b0f2d3a35b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2019-10-16T15:02:48Z (GMT) No. of bitstreams: 2 Artigo - Rosane Ribeiro Figueiredo Alves - 2013.pdf: 484238 bytes, checksum: 9b42ea9ac32099a21c09b8b0f2d3a35b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-10-16T15:02:48Z (GMT). No. of bitstreams: 2 Artigo - Rosane Ribeiro Figueiredo Alves - 2013.pdf: 484238 bytes, checksum: 9b42ea9ac32099a21c09b8b0f2d3a35b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013Background: The epidemiology of infection with multiple human papillomavirus (HPV) types in female adolescents is poorly understood. The purpose of this study was to explore the epidemiology of infection with multiple HPV types in adolescents and its association with demographic, behavioral and biological variables, as well as with cytological abnormalities. Methods: This community-based study included 432 sexually active females between 15 and 19 years of age. Genotyping for 30 HPV types was performed using a reverse blot strip assay/restriction fragment length polymorphism. Unconditional multivariate logistic regression was performed to identify factors significantly associated with HPV infection. The association between HPV infection and cytological abnormalities was calculated using a prevalence ratio. Results: The most common HPV types detected were 16, 51, 31, 52 and 18. Of the 121 HPV-positive women, 54 (44.6%) were infected with multiple HPV types. Having more than one lifetime sexual partner was associated with infection with any HPV infection, single HPV infection, and infection with multiple HPV types. The presence of cytological abnormalities was associated with infection with multiple HPV types. Conclusions: Co-infecting HPV genotypes occur in a high proportion of sexually active adolescents. Socio-demographic or sexual behavior factors associated with single HPV infection were similar to those associated with multiple HPV types. The higher risk of cytological abnormalities conferred by infection with multiple HPV types suggests a potential role of co-infection in the natural history of HPV infection