Characterization and genomic analysis of Exiguobacterium alkaliphilum B-3531D, an efficient crude oil degrading strain

The aim of the work was to carry out the physiological, biochemical and genetic characterization of the Exiguobacterium alkaliphilum B-3531D strai

A blue-purple pigment-producing bacterium isolated from the Vezelka river in the city of Belgorod

The purpose of the present work was to study the properties of a newly isolated bacterium capable of synthesizing blue-purple pigment. An aboriginal bacterium was isolated from the coastal zone of the Vezelka River in the city of Belgorod. Based on chemical and spectrophotometric studies of the crude ethanol extract, the pigment was identified as violacein, and the isolate was assigned to the group of violacein-forming bacteria, which includes bacteria of the genera Chromobacterium, Iodobacter, Janthinobacterium, Duganella, Collimonas, and Massili

Assessment of the antifungal activity of the violacein-forming strain Janthinobacterium sp. B-3515 against the mould fungus Alternaria brassicicola F-1864

A study of antifungal properties of violacein-forming strain Janthinobacterium sp. B-3515 as well as its secondary metabolite, violacein, against Alternaria brassicicola F-1864 is presented. Regardless of the presence of bacteria, mycelium growth in the first two days proceeded at the same rate. The effect of the bacterial strain was manifested after the third day of incubation. In general, during co-culture, the bacterial strain statistically significantly reduced the average growth of the mycelium of the mould fungus by 10

Експериментальне дослідження проблем детектування закладних пристроїв засобами нелінійної локації

It is common knowledge that today information is the most important resource of the modern world. Active development of information technologies and their integration, determine the extremely important roles that they play in many spheres of human life. Important information of national importance should be protected from unauthorized interception and the special importance in the issue of information security is the problem of information leakage through the mortgage devices. The problem lies in the fact that such devices for data capture can use most of the existing leakage channels. However, bookmarks are often used to intercept acoustic (linguistic) and specific information. Therefore, knowledge of the structural and technical features of the construction and functioning of hardware bookmarks is necessary to identify their strengths and weaknesses and to choose the optimal method of counteraction. The experimental researches of problems of detection of the mortgaged devices by a nonlinear locator are carried out. Laboratory studies were carried out using different semiconductor elements with different lengths of legs. The obtained results have shown that the length of the legs of semiconductor elements affects their detection.Проведены экспериментальные исследования проблем обнаружения закладных устройств нелинейным локатором. Лабораторные исследования проводились при использовании различных полупроводниковых элементов с различной длиной ножек. Полученные результаты показали, что длина ножек полупроводниковых элементов влияет на их детектирование.Проведено експериментальні дослідження проблем виявлення закладних пристроїв нелінійним локатором. Лабораторні дослідження проводилися при використанні різних напівпровідникових елементів з різною довжиною ніжок. Отримані результати показали, що довжина ніжок напівпровідникових елементів впливає на їхнє детектування

Whole-Genome Sequencing and Biotechnological Potential Assessment of Two Bacterial Strains Isolated from Poultry Farms in Belgorod, Russia

Bacteria, designated as A1.1 and A1.2, were isolated from poultry waste based on the ability to form ammonia on LB nutrient medium. Whole genome sequencing identified the studied strains as Peribacillus frigoritolerans VKM B-3700D (A1.1) and Bacillus subtilis VKM B-3701D (A1.2) with genome sizes of 5462638 and 4158287 bp, respectively. In the genome of B. subtilis VKM B-3701D, gene clusters of secondary metabolites of bacillin, subtilisin, bacilisin, surfactin, bacilliacin, fengycin, sactipeptide, and ratipeptide (spore killing factor) with potential antimicrobial activity were identified. Clusters of coronimine and peninodin production genes were found in P. frigoritolerans VKM B-3700D. Information on coronimine in bacteria is extremely limited. The study of the individual properties of the strains showed that the cultures are capable of biosynthesis of a number of enzymes, including amylases. The B. subtilis VKM V-3701D inhibited the growth of bacterial test cultures and reduced the growth rate of the mold fungus Aspergillus unguis VKM F-1754 by 70% relative to the control. The antimicrobial activity of P. frigoritolerans VKM V-3700D was insignificant. At the same time, a mixture of cultures P. frigoritolerans VKM B-3700D/B. subtilis VKM B-3701D reduced the growth rate of A. unguis VKM F-1754 by 24.5%. It has been shown that strain A1.1 is able to use nitrogen compounds for assimilation processes. It can be assumed that P. frigoritolerans VKM V-3700D belongs to the group of nitrifying or denitrifying microorganisms, which may be important in developing methods for reducing nitrogen load and eutrophication

A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod

Violacein is a biotechnologically significant secondary metabolite due to its antibacterial, antifungal, and other properties. Isolation, research, and identification of violacein producing strains are of interest for the development of biotechnological processes, in order to enhance the biosynthesis of this compound. The purpose of the present work was to study the properties of a newly isolated bacterium capable of synthesizing blue-purple pigment. An aboriginal bacterium was isolated from the coastal zone of the Vezelka River in the city of Belgorod. Based on chemical and spectrophotometric studies of the crude ethanol extract, the pigment was identified as violacein, and the isolate was assigned to the group of violacein-forming bacteria, which includes bacteria of the genera Chromobacterium, Iodobacter, Janthinobacterium, Duganella, Collimonas, and Massilia. Based on cultural, morphological, tinctorial, physiological, and biochemical properties, as well as analysis of the 16S rRNA gene sequence, the new isolated strain was assigned to the genus Janthinobacterium. The isolated strain is capable of suppressing the growth of a number of fungal and bacterial phytopathogens. For representatives of the genus Janthinobacterium, their inhibitory influence on cyanobacteria was shown for the first time