Logistic regression analysis of SNPs in ARMS2 and HTRA1 between AMD and PCV.

<p>P value and OR (95%CI) of rs10490924 and rs11200638 after adjusting for the following SNPs.</p

Summary of flow cytomery data of apoSptosis measured by Annexin V and PI.

<p>Summary of flow cytomery data of apoSptosis measured by Annexin V and PI.</p

Effect of wild-type ARMS2 and rs10490924 on the tube formation of RF/6A cells.

<p>PReceiver-M29-Basic plasmid-treated RF/6A cells (A), wild-type ARMS2 plasmid-treated cells RF/6A cells (B) and rs10490924 plasmid -treated RF/6A cells (C) were plated on Matrigel as described in Methods. After 24 h of incubation, the three groups cells formed well organized capillary-like structures. Values are the means±SD of at least three independent experiments. There are no significant differenence during the three groups (D, p>0.05). Abbreviations: wild-type ARMS2 plasmid-treated cells (WT); rs10490924 plasmid -treated cells (G270T); pReceiver-M29-Basic plasmid-treated cells (Vector).</p

Effect of wild-type ARMS2 and rs10490924 on the apoptosis of human RPE cells.

<p>Apoptosis was quantified by flow cytometry measured by Annexin V and PI staining. Data are presented as mean±SEM.Each experiment was repeated at least three independent times. DMEM+10%FBS control was set to 100%.*P<0.05. UR: late apoptotic cells; LR: early apoptotic cells, UR+LR: apoptotic cells. Abbreviations: wild-type ARMS2 plasmid-treated cells (WT); rs10490924 plasmid -treated cells (G270T); pReceiver-M29-Basic plasmid-treated cells (Vector).</p

Characteristics of the study population.

<p>Characteristics of the study population.</p

Effects of wild-type ARMS2 and rs10490924 on the cell cycles of human RPE cells.

<p>A) Cell cycle of pReceiver-M29-Basic plasmid-treated ARPE-19 cells. B) Cell cycle of wild-type ARMS2 plasmid-treated ARPE-19 cells. C) Cell cycle of rs10490924 plasmid-treated ARPE-19 cells. D) Data from the ARPE-19 cell cycle distribution of the control group, wild-type ARMS2 and rs10490924 group. Flow cytometric analysis demonstrates the effects of wild-type ARMS2 and rs10490924 on the human RPE cell cycle. The x-axis represents fluorescence intensity on a logarithmic scale and the y-axis represents the number of events. The results show that the fraction of cells in the G1 phase has decreased and the proportion of cells in the S phase has increased in the presence of wild-type ARMS2 and rs10490924-treated cells(*P<0.05, **P<0.01). Values are the mean±SD from three independent experiments. Abbreviations: wild-type ARMS2 plasmid-treated cells (WT); rs10490924 plasmid -treated cells (G270T); pReceiver-M29-Basic plasmid-treated cells (Vector).</p

Effects of wild-type ARMS2 and rs10490924 on the attachment of RF/6A and RPE cells.

<p>Cell attachment was assessed after 6 h incubation and subsequent MTT assay. Values are the means±SD of at least three independent experiments. Asterisks denote values significantly different from those of cells treated with wild-type ARMS2 and rs10490924 compared to negative control (p<0.01). Abbreviations: wild-type ARMS2 plasmid-treated cells (WT); rs10490924 plasmid -treated cells (G270T); pReceiver-M29-Basic plasmid-treated cells (Vector) (*P<0.05, **P<0.01).</p

Effect of wild-type ARMS2 and rs10490924 on the migration of RF/6A and human RPE cells.

<p>The migratory activity of both cell lines was estimated based on the number of cells that had migrated through the filter of the chamber. A) Migrated cells of pReceiver-M29-Basic plasmid-treated RF/6A cells. B) Migrated cells of wild-type ARMS2 plasmid-treated RF/6A cells. C) Migrated cells of rs10490924 plasmid -treated RF/6A cells. Values are the means±SD of at least three independent experiments. D) The results showed that the number of migrating cells in the wild-type ARMS2 plasmid -treated group was the most during the three groups(p<0.01). Abbreviations: wild-type ARMS2 plasmid-treated cells (WT); rs10490924 plasmid -treated cells (G270T); pReceiver-M29-Basic plasmid-treated cells (Vector) (*P<0.05, **P<0.01).</p

Polymorphisms in the <i>ARMS2</i> gene lesion: Distribution and Allele in neovascular Age-Related Macular Degeneration (nAMD), Polypoidal Choroidal Vasculopathy, and Controls in the northern Chinese Population.

<p>After Bonferroni correction the represent significance at P<0.05/8 = 0.00625.</p>*<p>Homozygous;Comparing the likelihood of individuals with two copies of the risk allele versus individuals with no copies of the risk allele;</p>**<p>Hetrozygous;Comparing the likelihood of individuals with one copy of the risk allele versus individuals with no copies of the risk allele;</p>$<p>homozygous for the risk factor.</p>##<p>HE: Hetrozygous insertion.</p>###<p>HO: Homozygous insertion.</p

Analysis of pair-wise LD across <i>ARMS2</i> and <i>HTRA1</i> SNPs in northern Chinese PCV and nAMD cohort.

<p>A) Analysis of pair-wise LD across the eight <i>ARMS2</i> and <i>HTRA1</i> SNPs in northern Chinese PCV cohort. B) Analysis of pair-wise LD across the eight <i>ARMS2</i> and <i>HTRA1</i> SNPs in northern Chinese nAMD cohort. The relative physical position of each SNP is given in the upper diagram. The pairwise D′ between all SNPs is given below each SNP combination. And when D′ = 1.0, no number is given inside the square. Bright red squares indicate D′≥0.90 and LOD≥2. Bright red squares indicate D′<0.90 and LOD≥2. White squares indicate D′<0.90 and LOD<2.</p