Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis.

BACKGROUND:Molecular diagnostic tests, notably polymerase chain reaction (PCR), are highly sensitive test for Leishmania detection, which is especially relevant in chronic cutaneous lesion with lower parasite load. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. Nevertheless, diagnosis of cutaneous leishmaniasis (CL) is hampered by the absence of a reference standard. Assuming that the PCR-based molecular tools are the most accurate diagnostic method, the objective of this systematic review was to assess the diagnostic accuracy of PCR-based molecular tools in a meta-analysis of the published literature. METHODOLOGY/PRINCIPAL FINDINGS:A search of the published literature found 142 papers of which only 13 studies met the selection criteria, including conventional PCR, real-time PCR, Loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), polymorphism-specific PCR (PS-PCR). The sensitivities of the individual studies ranged from 61% to 100%, and specificities ranged from 11% to 100%. The pooled sensitivities of PCR in smears were 0.95 (95% CI, 0.90 to 0.98), and the specificity was 0.91(95% CI, 0.70 to 0.98). In general population, estimates were lower in aspirates, skin biopsies and swab samples with 0.90 (95% CI, 0.80 to 0.95) and 0.87 (95% CI, 0.76 to 0.94) for sensitivity and specificity, respectively. The specificity was lower in consecutive studies, at 0.88 (95% CI, 0.59 to 0.98) and its CI were wider. CONCLUSIONS/SIGNIFICANCE:No statistically significant differences between the accuracy in smears, aspirate, skin biopsies or swabs samples were observed. Therefore, a simple smear sample run by PCR, instead more invasive samples, may be enough to obtain a positive diagnosis of CL. The results for PCR in all samples type confirm previous reports that consider PCR as the most accurate method for the diagnosis of CL

La inteligencia emocional aplicada a la administración de riesgo en la fábrica de calcetines crystal s. a. en el área de tejido sabaneta.

Identificar la importancia de la autoevaluación como práctica de la inteligencia emocional en la preparación del personal como parte de un proceso de autoconocimiento, autorregulación y motivación que favorezca el mejoramiento de las relaciones interpersonales, la responsabilidad, el compromiso, la capacidad para solucionar conflictos, la aplicación de los principios éticos en la vida laboral y familiar optimizando el aprovechamiento de los recursos como una estrategia en la gerencia de riesgo. Evaluar a través de una guía de autoevaluación, el estado actual del personal en la Fábrica de Calcetines Crystal S.A. en el área de tejido Sabaneta, determinando su estado de inteligencia emocional, teniendo en cuenta las respuestas afirmativas dadas por cada uno de los encuestados, tomando como base los indicadores establecidos para: el autoconocimiento, la autorregulación, la motivación, la empatía y las habilidades sociales y su incidencia a través de la teoría de la gerencia del riesgo. Proponer un programa de aplicación de la inteligencia emocional a través de la sensibilización y la autoevaluación para la detección oportuna de los estados emocionales de los trabajadores y los riesgos que afectan la producción y por medio de la administración del riesgo prevenir, orientar y solucionarlos. Obteniendo un ambiente laboral favorable para la calidad y la producción optimizando los recursos

Safety and efficacy of mesenchymal stem cells therapy in the treatment of rheumatoid arthritis disease: A systematic review and meta-analysis of clinical trials.

Background and objectivesSome patients have insufficient treatment response to conventional disease-modifying antirheumatic drugs (cDMARD); although biologics have proven to be an effective treatment for RA, the effects that bDMARDs have on integumentary, cardiac, and immune systems and the high costs associated with these treatments, make that mesenchymal stem cell-based therapies (MSCs) for RA are being considered potential treatment methods. This work analyses the performance in safety and efficacy terms of MSCs techniques.Methods and findingA literature search was performed in PubMed, EMBASE, Cochrane Library, Web of Science, and Open Grey databases from inception to October 28, 2022. Three randomized controlled trials (RCTs) and one non-randomized controlled trial (non-RCTs), including 358 patients met our inclusion criteria and were included in qualitative synthesis; only RCTs were eligible for quantitative synthesis (meta-analysis). Meta-analysis of adverse events (AE) in RCTs showed no significant differences in the incidence of AE in the MSCs group compared to the control group (Risk ratio: 2.35; 95% CI, 0.58 to 9.58; I2 = 58.80%). The pooled Risk ratio for non-serious and serious adverse events showed no statistical difference between intervention and control groups concerning the incidence of non-serious and serious adverse events (Risk ratio: 2.35; 95% CI, 0.58 to 9.51; I2 = 58.62%) and (Risk ratio: 1.10; 95% CI, 0.15 to 7.97; I2 = 0.0%) respectively. The Health Assessment Questionnaire (HAQ) and Disease Activity Score (DAS28) decreased in agreement with the decreasing values of C-reactive protein (CRP) and Erythrocyte sedimentation rate (ESR). Additionally, a trend toward clinical efficacy was observed; however, this improvement was not shown in the studies after 12 months of follow-up without continuous treatment administration.ConclusionThis Systematic review and meta-analysis showed a favorable safety profile, without life-threatening events in subjects with RA, and a trend toward clinical efficacy that must be confirmed through high-quality RCTs, considerable sample size, and extended follow-up in subjects with RA

In vitro and in vivo antileishmanial activity of Artemisia annua L. leaf powder and its potential usefulness in the treatment of uncomplicated cutaneous leishmaniasis in humans

ABSTRACT INTRODUCTION: Cutaneous leishmaniasis (CL) is a tropical disease that affects millions of individuals worldwide. The current drugs for CL may be effective but have serious side effects; hence, alternatives are urgently needed. Although plant-derived materials are used for the treatment of various diseases in 80% of the global population, the validation of these products is essential. Gelatin capsules containing dried Artemisia annua leaf powder were recently developed as a new herbal formulation (totum) for the oral treatment of malaria and other parasitic diseases. Here, we aimed to determine the usefulness of A. annua gel capsules in CL. METHODS: The antileishmanial activity and cytotoxicity of A. annua L. capsules was determined via in vitro and in vivo studies. Moreover, a preliminary evaluation of its therapeutic potential as antileishmanial treatment in humans was conducted in 2 patients with uncomplicated CL. RESULTS: Artemisia annua capsules showed moderate in vitro activity in amastigotes of Leishmania (Viannia) panamensis; no cytotoxicity in U-937 macrophages or genotoxicity in human lymphocytes was observed. Five of 6 (83.3%) hamsters treated with A. annua capsules (500mg/kg/day) for 30 days were cured, and the 2 examined patients were cured 45 days after initiation of treatment with 30g of A. annua capsules, without any adverse reactions. Both patients remained disease-free 26 and 24 months after treatment completion. CONCLUSION: Capsules of A. annua L. represent an effective treatment for uncomplicated CL, although further randomized controlled trials are needed to validate its efficacy and safety

Perfil de algunas condiciones psicosociales de un grupo de adultos mayores

Objetivo: exponer algunos componentes psicosociales de los adultos mayores de Caldas, Antioquia para identificar procesos protectores y causantes de deterioro del desarrollo humano y social de este colectivo. Metodología: esta investigación es un estudio descriptivo-transversal, de intervención, que tiene en cuenta elementos de la epidemiología crítica. Como técnica cuantitativa para la recolección de información, se realizó un muestreo multietápico, estratificado y en el componente cualitativo, un muestreo intencional. Resultado: aunque se encuentran varios procesos deteriorantes de la salud psicosocial de los adultos mayores, al aplicar una escala de depresión geriátrica con la que se puede tener un indicio de la condición mórbida por depresión, se encuentra un reducido número con ella. Conclusión: los adultos mayores de Caldas cuentan con mecanismos socioculturales compensadores que les permiten desarrollarse en el contexto caldeño haciendo frente a las vulnerabilidades para lograr un envejecimiento, si no exitoso, al menos adaptado a las adversidades. La vulnerabilidad identificada en este grupo de adultos mayores debe enriquecer el diagnóstico sobre sus condiciones para favorecer el desarrollo de políticas públicas orientadas a mejorar esos procesos deteriorantes; los procesos protectores identificados deben ser potenciados y favorecidos igualmente por los grupos intersectoriales, que deben aunar esfuerzos por la protección de los adultos mayores