Quasi maximum likelihood estimation for simultaneous spatial autoregressive models

This paper considers the problem of estimating a simultaneous spatial autoregressive model (SSAR). We propose using the quasi maximum likelihood method to estimate the model. The asymptotic properties of the maximum likelihood estimator including consistency and limiting distribution are investigated. We also run Monte Carlo simulations to examine the finite sample performance of the maximum likelihood estimator

Quasi maximum likelihood estimation for simultaneous spatial autoregressive models

This paper considers the problem of estimating a simultaneous spatial autoregressive model (SSAR). We propose using the quasi maximum likelihood method to estimate the model. The asymptotic properties of the maximum likelihood estimator including consistency and limiting distribution are investigated. We also run Monte Carlo simulations to examine the finite sample performance of the maximum likelihood estimator

Preparation and antitumor evaluation of hinokiflavone hybrid micelles with mitochondria targeted for lung adenocarcinoma treatment

Hinokiflavone (HF) is a natural biflavonoid extracted from medicinal plants such as Selaginella tamariscina and Platycladus orientalis. HF plays a crucial role in the treatment of several cancers. However, its poor solubility, instability, and low bioavailability have limited its use. In this study, soluplus/d-α-tocopherol acid polyethylene glycol 1000 succinate (TPGS)/dequalinium (DQA) was applied to improve the solubilization efficiency and stability of HF. HF hybrid micelles were prepared via thin-film hydration method. The physicochemical properties of micelles, including particle size, zeta potential, encapsulation efficiency, drug loading, CMC value, and stability were investigated. The in vitro cytotoxicity assay showed that the cytotoxicity of the HF hybrid micelles was higher than that of free HF. In addition, the HF hybrid micelles improved anticancer efficacy and induced mitochondria-mediated apoptosis, which is associated with the high levels of ROS inducing decreased mitochondrial membrane potential, promoting apoptosis of tumor cells. Furthermore, in vivo tumor suppression, smaller tumor volume and increased expression of pro-apoptotic proteins were found in nude mice treated with HF hybrid micelles, suggesting that HF hybrid micelles had stronger tumor suppressive activity compared with free HF. In summary, HF hybrid micelles developed in this study enhanced antitumor effect, which may be a potential drug delivery system for the treatment of lung adenocarcinoma

Preparation, evaluation and metabolites study in rats of novel amentoflavone-loaded TPGS/soluplus mixed nanomicelles

Amentoflavone (AMF) is a kind of biflavonoids existing in Ginkgo biloba leaves. It has many biological activities, such as antioxidant, anti-inflammatory, anti-bacterial, antiviral, hypoglycemic, anti-tumor and inducing apoptosis. However, its solubility and bioavailability are poor and there are a few studies on it in vivo. In this study, to improve its solubility and bioavailability, the nanomicelles were prepared with TPGS and soluplus as carriers for the first time. The particle size, Zeta potential, encapsulation efficiency, drug loading, stability, cytotoxicity, cellular uptake, and metabolites in rats were studied. Cytotoxicity, cellular uptake, and metabolites in rats of AMF-loaded TPGS/soluplus mixed micelles were compared with those of AMF. As a result, AMF-loaded TPGS/soluplus mixed micelles with a particle size of 67.33 ± 2.01 nm and Zeta potential of −0.84133 ± 0.041405 mV were successfully prepared. The encapsulation efficiency and drug loading of the mixed nanomicelles were 99.18 ± 0.76% and 2.47 ± 0.01%, respectively. The physical and chemical properties of the mixed micelles were stable within 60 d, and the cytotoxicity of the mixed micelles was much greater than that of AMF monomers. Thirty-four kinds of metabolites of AMF were identified in rats. The metabolites were mainly distributed in rat feces. No metabolites were detected in bile and plasma. 14 kinds of metabolites of the mixed micelles in rats were detected, including 11 in feces, 6 in urine, and 3 in plasma, which indicated that the bioavailability of AMF has been improved. And the toxicity to cancer cells was enhanced, which laid a foundation for the development of new drugs

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Eupatorin is the major bioactive component of Java tea (Orthosiphon stamineus), exhibiting strong anticancer and anti-inflammatory activities. However, no research on the metabolism of eupatorin has been reported to date. In the present study, ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) combined with an efficient online data acquisition and a multiple data processing method were developed for metabolite identification in vivo (rat plasma, bile, urine and feces) and in vitro (rat liver microsomes and intestinal flora). A total of 51 metabolites in vivo, 60 metabolites in vitro were structurally characterized. The loss of CH2, CH2O, O, CO, oxidation, methylation, glucuronidation, sulfate conjugation, N-acetylation, hydrogenation, ketone formation, glycine conjugation, glutamine conjugation and glucose conjugation were the main metabolic pathways of eupatorin. This was the first identification of metabolites of eupatorin in vivo and in vitro and it will provide reference and valuable evidence for further development of new pharmaceuticals and pharmacological mechanisms

Metabolomic Approach to Screening Homozygotes in Chinese Patients with Severe Familial Hypercholesterolemia

Homozygous familial hypercholesterolemia (HoFH) is a rare inborn-errors-of-metabolism disorder characterized by devastatingly elevated low-density lipoprotein cholesterol (LDL-C) and premature cardiovascular disease. The gold standard for screening and diagnosing HoFH is genetic testing. In China, it is expensive and is always recommended for the most likely HoFH subjects with aggressive LDL-C phenotype. However, the LDL-C levels of HoFH patients and a substantial proportion of heterozygous FH (HeFH) patients overlapped considerably. Here, we performed a cost-effective metabolomic profiling on genetically diagnosed HoFH (n = 69) and HeFH patients (n = 101) with overlapping LDL-C levels, aiming to discovery a unique metabolic pattern for screening homozygotes in patients with severe FH. We demonstrated a differential serum metabolome profile in HoFH patients compared to HeFH patients. Twenty-one metabolomic alterations showed independent capability in differentiating HoFH from severe HeFH. The combined model based on seven identified metabolites yielded a corrected diagnosis in 91.3% of HoFH cases with an area under the curve value of 0.939. Collectively, this study demonstrated that metabolomic profiling serves as a useful and economical approach to preselecting homozygotes in FH patients with severe hypercholesterolemia and may help clinicians to conduct selective genetic confirmation testing and familial cascade screening

EMR-YOLO: A Study of Efficient Maritime Rescue Identification Algorithms

Accurate target identification of UAV (Unmanned Aerial Vehicle)-captured images is a prerequisite for maritime rescue and maritime surveillance. However, UAV-captured images pose several challenges, such as complex maritime backgrounds, tiny targets, and crowded scenes. To reduce the impact of these challenges on target recognition, we propose an efficient maritime rescue network (EMR-YOLO) for recognizing images captured by UAVs. In the proposed network, the DRC2f (Dilated Reparam-based Channel-to-Pixel) module is first designed by the Dilated Reparam Block to effectively increase the receptive field, reduce the number of parameters, and improve feature extraction capability. Then, the ADOWN downsampling module is used to mitigate fine-grained information loss, thereby improving the efficiency and performance of the model. Finally, CASPPF (Coordinate Attention-based Spatial Pyramid Pooling Fast) is designed by fusing CA (Coordinate Attention) and SPPF (Spatial Pyramid Pooling Fast), which effectively enhances the feature representation and spatial information integration ability, making the model more accurate and robust when dealing with complex scenes. Experimental results on the AFO dataset show that, compared with the YOLOv8s network, the EMR-YOLO network improves the mAP (mean average precision) and mAP50 by 4.7% and 9.2%, respectively, while reducing the number of parameters and computation by 22.5% and 18.7%, respectively. Overall, the use of UAVs to capture images and deep learning for maritime target recognition for maritime rescue and surveillance improves rescue efficiency and safety

First Direct Dating of Alteration of Paleo-Oil Pools Using Rubidium-Strontium Pyrite Geochronology

Direct dating of petroleum systems by hydrocarbon or associated authigenic minerals is crucial for petroleum system analysis and hydrocarbon exploration. The precipitation of authigenic pyrite in petroliferous basins is commonly genetically associated with hydrocarbon generation, migration, accumulation, or destruction. Pyrite rubidium-strontium (Rb-Sr) isotope dilution thermal ionization mass spectrometry (ID-TIMS) is a well-developed technique, and its successful application for high-temperature ore systems suggests that this dating method has the potential to directly date key processes in the low-temperature petroleum systems. Rb-Sr data for pyrites in two Ordovician carbonate rock specimens collected from similar to 4952 m in the YD-2 well in the Yudong region, northern Tarim Basin (NW China), yield two identical isochron ages within analytical uncertainties: 206 +/- 13 (2 sigma) and 224 +/- 28 Ma (2 sigma). SEM investigations demonstrate that Rb and Sr atoms mainly reside in the crystal lattice of the pyrites due to the absence of fluid and mineral inclusions. The rigorous Rb-Sr isochron relations documented in the samples indicate that such residency can result in sufficient Rb/Sr fractionation and initial Sr isotopic homogenization for geochronology. In addition, the closure temperature (227-320 degrees C) for the Rb-Sr isotope system in pyrite is higher than the precipitation temperature for pyrite in petroleum-related environments (below 200 degrees C), suggesting that the Rb-Sr age of pyrite was not overprinted by post-precipitation alteration. Integrating the lead-strontium-sulfur isotopes of the pyrites with burial history analysis, the ages are interpreted as the timing of alteration of the paleo-oil pool by a hydrothermally-triggered thermochemical sulfate reduction process. This study, for the first time, demonstrates that Rb-Sr pyrite geochronology, combined with radiogenic and stable isotopic analyses, can be a useful tool to evaluate the temporal evolution of oil pools. This approach bears great potential for dating of petroleum systems anywhere else in the world

Fermentation characteristics of different sources of dietary fiber in vitro and impacts on growth performance, nutrient digestibility and blood para-meters of piglets

This study aimed to assess the effect of various source of high-fiber ingredients on growth performance, apparent digestibility of nutrients, and blood para- meters of piglets by investigating their in vitro fermentation characteristics. A total of 300 healthy crossbred piglets were randomly allocated to one of five diets including corn-soybean meal basal diet (CON), sugar beet pulp (SBP), soybean hull (SBH), fermented distillers’ grains (FDG), and kelp powder (KP). In vitro fermentation analysis revealed that the predominant short-chain fatty acids produced were acetate and valerate. Sugar beet pulp and kelp powder exhibited rapid fermentation rates, in contrast to SBH, oat bran, and FDG. The apparent total tract digestibility of dry matter (P < 0.05), gross energy (P < 0.05), and crude protein were decreased (P < 0.001) in different fiber diets than the CON diet. The serum concentration of threonine was increased by feeding fibers diets (P < 0.05). In conclusion, a diet containing 3% SBP (with a soluble-to-insoluble dietary fiber ratio of 17.04%) showed the best effects on increasing nutrients digestibility and serum amino acid concentrations. These findings may inform future applications of SBP in piglet nutrition

Secondary Migration of Hydrocarbons in the Zhujiang Formation in the Huixi Half-graben, Pearl River Mouth Basin, South China Sea

The process and mechanisms of secondary hydrocarbon migration in the Huixi half-graben, Pearl River Mouth Basin, were investigated on the basis of geological analysis of the strata and study of the porosity and permeability of the reservoir rocks, fluid potential, oil properties, and geochemistry of oil-source correlation. The results suggest that the hydrocarbons of the Zhujiang Formation in the Huixi half-graben were derived from source rocks of the Eocene Wenchang Formation and the Eocene–Oligocene Enping Formation in the Huizhou Sag. The hydrocarbons migrated laterally from northeast to southwest. The sandstone in the upper member of the Zhujiang Formation exhibited superior physical properties (porosity and permeability) and connectivity than the lower member. Thin sandstone beds with good physical properties and stable distribution in the upper member of the Zhujiang Formation were the main carrier beds for lateral hydrocarbon migration.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author