Inclusion of Cocoa Bean Shell in the Diet of Dairy Goats: Effects on Milk Production Performance and Milk Fatty Acid Profile

The use of agro-industrial by-products in animal nutrition is a promising strategy to reduce the food-feed competition, the diet cost at farm level and the environmental impact of animal-derived food production. In this study, the suitability of cocoa bean shell (CBS), a by-product of the cocoa industry, as a feed ingredient in the diet of dairy goats was evaluated, with a focus on the related implications on feed intake, milk yield, milk main constituents, and fatty acid (FA) profile of milk fat. Twenty-two Camosciata delle Alpi goats were divided into two balanced groups. All the goats were fed mixed hay ad libitum. The control group (CTRL; n = 11) also received 1.20 kg/head × day of a commercial concentrate, while in the experimental group (CBS; n = 11) 200 g of the CTRL concentrate were replaced by the same amount of pelleted CBS. The total dry matter intake of the goats was reduced by the dietary inclusion of CBS (P ≤ 0.01). The milk yield, as well as the milk fat, protein, and casein contents and yields were unaffected by the treatment. Milk from the CBS-fed goats showed decreased urea content when compared to the CTRL group (P ≤ 0.001). Milk from the CBS group of goats also showed increased concentrations of total branched-chain FA (both iso and anteiso forms; P ≤ 0.001) and total monounsaturated FA (P ≤ 0.05), as well as a decreased ∑ n6/∑ n3 FA ratio (P ≤ 0.05). De novo saturated FA, total polyunsaturated FA, total conjugated linoleic acids, and the majority of ruminal biohydrogenation intermediates remained unaffected by the dietary treatment. These results suggest that CBS can be strategically used as an alternative non-conventional raw material in diets intended for lactating goats, with no detrimental effects on their milk production performance. The use of CBS in goat nutrition may be hindered by the presence of theobromine, a toxic alkaloid. Special attention is needed by nutritionists to avoid exceeding the theobromine limits imposed by the current legislation. Detheobromination treatments are also suggested in literature to prevent toxic phenomena

Full-fat insect meals in ruminant nutrition: in vitro rumen fermentation characteristics and lipid biohydrogenation

BACKGROUND: The most used protein sources in ruminant nutrition are considered as having negative impacts in terms of environmental sustainability and competition with human nutrition. Therefore, the investigation of alternative and sustainable feedstuffs is becoming a priority in ruminant production systems. RESULTS: This trial was designed to evaluate eight full-fat insect meals (Acheta domesticus – ACD; Alphitobius diaperinus – ALD; Blatta lateralis – BL; Gryllus bimaculatus – GB; Grylloides sygillatus – GS; Hermetia illucens – HI; Musca domestica – MD; and Tenebrio molitor – TM) as potential protein and lipid sources in ruminant nutrition. Fermentation parameters and fatty acids (FA) of rumen digesta after 24-h in vitro ruminal incubation of the tested insect meals were measured and compared with those of three plant-based meals (soybean meal, rapeseed meal and sunflower meal) and fishmeal (FM). Similarly to FM, the insect meals led to a significantly lower total gas production (on average, 1.75 vs. 4.64 mmol/g dry matter—DM), methane production (on average, 0.33 vs. 0.91 mmol/g DM), volatile FA production (on average, 4.12 vs. 7.53 mmol/g DM), and in vitro organic matter disappearance (on average, 0.32 vs. 0.59 g/g) than those observed for the plant meals. The insect meals also led to lower ammonia of rumen fluid, when expressed as a proportion of total N (on average, 0.74 vs. 0.52 for the plant and insect meals, respectively), which could be an advantage provided that intestinal digestibility is high. Differences in ruminal fermentation parameters between the insect meals could be partially explained by their chitin, crude protein and ether extract contents, as well as by their FA profile. In particular, high content of polyunsaturated FA, or C12:0 (in HI), seems to partially inhibit the ruminal fermentations. CONCLUSIONS: The tested full-fat insect meals appear to be potentially an interesting protein and lipid source for ruminants, alternative to the less sustainable and commonly used ones of plant origin. The FA profile of the rumen digesta of ACD, ALD, GB, GS and TM, being rich in n-6 polyunsaturated FA, could be interesting to improve the quality of ruminant-derived food products. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-022-00792-2

Hazelnut Skin in Ewes’ Diet: Effects on Colostrum Immunoglobulin G and Passive Transfer of Immunity to the Lambs

SIMPLE SUMMARY: A ewe’s diet in the last period of gestation can modify the immunoglobulin G composition of the colostrum and consequently the health status of lambs. This study aims to determine the role of hazelnut skin on the immunological colostrum quality and the passive immunity transfer in newborns. The results show that hazelnut skin supplementation in the diet positively affects the immunoglobulin G composition of the colostrum. The experimental trial underlines that the use of by-products in livestock feeding provides a paramount opportunity to create a circular economy system with health benefit on farmed animals. ABSTRACT: Passive immunity transfer has a pivotal role in newborn lambs, where the colostrum represents the primary source of immunoglobulins. This study hypothesized that the high content in polyphenolic compounds, mono- and poly-unsaturated fatty acids, and vitamin E of hazelnut skin affects blood and colostrum immunoglobulin G (IgG) concentration and related gamma-glutamyl-transferase (GGT) and lactate dehydrogenase (LDH) levels in sheep and their lambs. In the last 45 days of pregnancy, ewes were divided into a control (CTR) and a hazelnut skin supplemented group (HZN). Blood and colostrum were collected from ewes and lambs before the first suckling, at 24 and 48 h after birth, then IgG concentration, GGT and LDH activity levels were measured. IgG concentration in the colostrum and in lamb’s serum were significantly greater in HZN than CTR. No significant difference was detected for ewe’s blood. A significant positive correlation was found between IgG and GGT in lambs’ serum and colostrum, between IgG and LDH, as well as between GGT and LDH in lambs’ serum and colostrum. Our results suggest that hazelnut skin supplementation influences IgG colostrum concentration, with improved immune passive transfer to the suckling lambs. The transfer of maternal derived immune factors is confirmed by the GGT and LDH enzyme activity levels

Fatty acid composition of the seed oils of selected Vicia L. taxa from Tunisia

Whole mature seeds of eight selected varieties, subspecies and accessions of three Vicia L. species grown in Tunisia were investigated for their fatty acid (FA) profile. The FA composition ranged from lauric (C12:0) to lignoceric (C24:0) acids. The total FA content was 1235.14 to 1580.34 mg 100 g–1 dry matter (DM). Linoleic acid (C18:2 c9c12; 647.87 to 801.93 mg 100 g–1 DM, i.e. >50% of total FA), oleic acid (C18:1 c9; 181.32 to 346.79 mg 100 g–1 DM, i.e. 13.2 to 24.6% of total FA) and a-linolenic acid (C18:3 c9c12c15; 42.01 to 97.72 mg 100 g–1 DM, i.e. 3.4 to 7.1% of total FA) were the most abundant unsaturated FA. Palmitic acid (C16:0; 189.86 to 281.07 mg 100 g–1 DM, i.e. 15.4 to 17.8% of total FA) and stearic acid (C18:0; 24.35 to 52.75 mg 100 g–1 DM, i.e. 2.0 to 4.0% of total FA) were the major saturated ones. The sum of all other FA did not exceed 3.0% of TFA. The favourable FA profile of the studied vetch seeds makes them interesting cheap diet components to be used in the nutrition of ruminants and non-ruminants reared in the dryland agricultural regions of Mediterranean countries