Gene profiling of cell cycle progression through S-phase reveals sequential expression of genes required for DNA replication and nucleosome assembly

The ordered expression of genes after growth factor stimulation in G(1) supportsthe onset of DNA replication. To characterize regulatory events during S-phase when cell cycle progression has become growth factor independent, we have profiled the expression of over 7,000 human genes using GeneChip DNA microarray analysis. HeLa cells were synchronized at the beginning of S-phase by thymidine/aphidicolin block, and RNA populations were analyzed throughout the S and G(2) phases. Expression of genes involved in DNA replication is maximal during early S-phase, whereas histone mRNAs peak at mid S-phase. Genes related to cell proliferation, including those encoding cyclins, oncoproteins, growth factors, proteins involved in signal transduction, and DNA repair proteins, follow distinct temporal patterns of expression that are functionally linked to initiation of DNA replication and progression through S-phase. The timing of expression for many genes in tumor-derived HeLa cells is highly conserved when compared with normal cells. In contrast, a number of genes show growth phenotype-related expression patterns that may directly reflect loss of stringent growth control in tumor cells. Our data reveal there is a core subset of cell growth-related genes that is fundamental to cycling cells irrespective of cell growth phenotype

Gattini 2010: Cutting Edge Science at the Bottom of the World

The high altitude Antarctic sites of Dome A and the South Pole offer intriguing locations for future large scale optical astronomical Observatories. The Gattini project was created to measure the optical sky brightness, large area cloud cover and aurora of the winter-time sky above such high altitude Antarctic sites. The Gattini-DomeA camera was installed on the PLATO instrument module as part of the Chinese-led traverse to the highest point on the Antarctic plateau in January 2008. This single automated wide field camera contains a suite of Bessel photometric filters (B, V, R) and a long-pass red filter for the detection and monitoring of OH emission. We have in hand one complete winter-time dataset (2009) from the camera that was recently returned in April 2010. The Gattini-South Pole UV camera is a wide-field optical camera that in 2011 will measure for the first time the UV properties of the winter-time sky above the South Pole dark sector. This unique dataset will consist of frequent images taken in both broadband U and B filters in addition to high resolution (R similar to 5000) long slit spectroscopy over a narrow bandwidth of the central field. The camera is a proof of concept for the 2m-class Antarctic Cosmic Web Imager telescope, a dedicated experiment to directly detect and map the redshifted lyman alpha fluorescence or Cosmic Web emission we believe possible due to the unique geographical qualities of the site. We present the current status of both projects

Photometry of Variable Stars from Dome A, Antarctica

Dome A on the Antarctic plateau is likely one of the best observing sites on Earth thanks to the excellent atmospheric conditions present at the site during the long polar winter night. We present high-cadence time-series aperture photometry of 10,000 stars with i<14.5 mag located in a 23 square-degree region centered on the south celestial pole. The photometry was obtained with one of the CSTAR telescopes during 128 days of the 2008 Antarctic winter. We used this photometric data set to derive site statistics for Dome A and to search for variable stars. Thanks to the nearly-uninterrupted synoptic coverage, we find 6 times as many variables as previous surveys with similar magnitude limits. We detected 157 variable stars, of which 55% are unclassified, 27% are likely binaries and 17% are likely pulsating stars. The latter category includes delta Scuti, gamma Doradus and RR Lyrae variables. One variable may be a transiting exoplanet.Comment: Accepted for publication in the Astronomical Journal. PDF version with high-resolution figures available at http://faculty.physics.tamu.edu/lmacri/papers/wang11.pd

Airglow and Aurorae at Dome A, Antarctica

Despite the absence of artificial light pollution at Antarctic plateau sites such as Dome A, other factors such as airglow, aurorae, and extended periods of twilight have the potential to adversely affect optical observations. We present a statistical analysis of the airglow and aurorae at Dome A using spectroscopic data from Nigel, an optical/near-IR spectrometer operating in the 300–850 nm range. These data complement photometric images from Gattini, a wide-field (90°) CCD camera with B, V, and R filters, allowing the background sky brightness to be disentangled from the various airglow and auroral emission lines. The median auroral contribution to the B, V, and R photometric bands is found to be 22.9, 23.4, and 23.0 mag arcsec^(-2), respectively. Auroral emissions most frequently occur between 10–23 hr local time, when up to 50% of observations are above airglow-level intensities. While infrequent, the strongest emissions detected occurred in the hours just prior to magnetic midnight. We are also able to quantify the amount of annual dark time available as a function of wavelength, as well as in the standard BVR photometric bands. On average, twilight ends when the Sun reaches a zenith distance of 102.6°

PHOTOMETRY OF VARIABLE STARS FROM DOME A, ANTARCTICA: RESULTS FROM THE 2010 OBSERVING SEASON

We present results from a season of observations with the Chinese Small Telescope ARray (CSTAR), obtained over 183 days of the 2010 Antarctic winter. We carried out high-cadence time-series aperture photometry of 20,000 stars with i<15.3 mag located in a 23 square-degree region centered on the south celestial pole. We identified 188 variable stars, including 67 new objects relative to our 2008 observations, thanks to broader synoptic coverage, a deeper magnitude limit and a larger field of view. We used the photometric data set to derive site statistics from Dome A. Based on two years of observations, we find that extinction due to clouds at this site is less than 0.1 and 0.4 mag during 45% and 75% of the dark time, respectively.Comment: Accepted for publication in the Astronomical Journal. Light curves and finding charts of all variable stars will be made available at http://casdc.china-vo.org/data/csta

STELLAR VARIABILITY AND FLARE RATES FROM DOME A, ANTARCTICA, USING 2009 AND 2010 CSTAR OBSERVATIONS

The Chinese Small Telescope ARray (CSTAR) carried out high-cadence time-series observations of 20.1 square degrees centered on the South Celestial Pole during the 2008, 2009 & 2010 winter seasons from Dome A in Antarctica. The nearly-continuous 6 months of dark conditions during each observing season allowed for >10^6 images to be collected through gri and clear filters, resulting in the detection of >10^4 sources over the course of 3 years of operation. The nearly space-like conditions in the Antarctic plateau are an ideal testbed for the suitability of very small-aperture (<20 cm) telescopes to detect transient events, variable stars and stellar flares. We present the results of a robust search for such objects using difference image analysis of the data obtained during the 2009 & 2010 winter seasons. While no transients were found, we detected 29 flaring events and find a normalized flaring rate of 5+\-4x10^-7 flare/hour for late-K dwarfs, 1+\-1x10^-6 flare/hour for M dwarfs and 7+\-1x10^-7 flare/hour for all other stars in our sample. We suggest future small-aperture telescopes planned for deployment at Dome A would benefit from a tracking mechanism, to help alleviate effects from ghosting, and a finer pixel scale, to increase the telescope's sensitivity to faint objects. We find that the light curves of non-transient sources have excellent photometric qualities once corrected for systematics, and are limited only by photon noise and atmospheric scintillation.Comment: Accepted for Publication in the Astronomical Journal, 14 pages, 12 figures and 2 table

Winter sky brightness and cloud cover at Dome A, Antarctica

At the summit of the Antarctic plateau, Dome A offers an intriguing location for future large scale optical astronomical observatories. The Gattini Dome A project was created to measure the optical sky brightness and large area cloud cover of the winter-time sky above this high altitude Antarctic site. The wide field camera and multi-filter system was installed on the PLATO instrument module as part of the Chinese-led traverse to Dome A in January 2008. This automated wide field camera consists of an Apogee U4000 interline CCD coupled to a Nikon fisheye lens enclosed in a heated container with glass window. The system contains a filter mechanism providing a suite of standard astronomical photometric filters (Bessell B, V, R) and a long-pass red filter for the detection and monitoring of airglow emission. The system operated continuously throughout the 2009, and 2011 winter seasons and part-way through the 2010 season, recording long exposure images sequentially for each filter. We have in hand one complete winter-time dataset (2009) returned via a manned traverse. We present here the first measurements of sky brightness in the photometric V band, cloud cover statistics measured so far and an estimate of the extinction