Discussing the Construction of Gratefulness Education in Modern Times

Gratefulness is Chinese traditional virtue, which gives Chinese the power to go upper ,encourages Chinese to understand others, having a important meaning to cultivate personal character, perfect the life meaning. In addition to this, it is necessary to build the harmonious camp and society. However, at present as a necessary part of the students’ education in university, it is also weak. There is no delay to strengthen the gratefulness education. This dissertation aims at probing into the reasons of loss of gratitude feeling, thereafter puts forward the countermeasures for this issue

Analysing the Effectiveness of University Scientific Research Projects on the Knowledge Management

This paper briefly analyzes the connotation and characteristic of knowledge management. Elaborating the problems which exist in the present scientific research project management, analyzing the relationship between university scientific research project management and knowledge management; putting forward the knowledgeable, informatization, modernization and humanization strategy into the process of scientific research project management under the view of knowledge management, and exploring theory and method to make full use of knowledge management, establishing the comprehensive and whole process management mechanism in local university scientific research project management, so as to improve the efficiency of scientific research project management, promoting the development of local colleges and universities’ scientific research

Characteristics of University’s Scientific Research Project Management i n the New Times

Along with the comprehensive deepening of reform and open, China\u27s economic has developed rapidly. National and provincial’s longitudinal and transverse scientific research projects are increasing. In particular, our country has many universities and they have close relationship with economics’ development. Also, scientific research projects that involved are multitude of names. Understand our country’s characteristics of scientific research project i n the new period correctly. Adjust local university’s scientific research project management method, means and key in time, and make out corresponding management system to promote local universities’ further development

Down-regulated miR-9 and miR-433 in human gastric carcinoma

<p>Abstract</p> <p>Background</p> <p>MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.</p> <p>Methods</p> <p>To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.</p> <p>Results</p> <p>We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.</p> <p>Conclusion</p> <p>Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.</p

Association between admission hemoglobin level and prognosis in sepsis patients based on a critical care database

Abstract This study aimed to explore the association between admission hemoglobin level and clinical outcomes in sepsis based on Medical Information Mart for Intensive Care IV(MIMIC-IV) database. It was a retrospective study. Sepsis patients in the database were included. Data extraction from MIMIC-IV was performed by PostgreSQL 9.6 software. Three different models including crude model (adjusted for none), model I (adjusted for gender and age) and model II (adjusted for all potential cofounders) were constructed. A generalized liner model and a smooth fitting curve for indicating the relationship between hemoglobin level and 30-day mortality were performed. 6249 septic patients with a 30.18% of 30-day mortality were included. With 1 g/dl increment in hemoglobin level, the values of odds ratio (OR) in crude model, model I and model II were 0.96 (95% confidential interval (CI) 0.94–0.99, P = 0.0023), 0.96 (95%CI 0.93–0.98, P = 0.0010) and 0.87 (95%CI 0.79–0.95, P = 0.0020), respectively. The smooth fitting curve indicated a non-linear relationship and the turning point was 7.2 g/dl. Compared the hemoglobin group < 7.2 g/dl, the risk of 30-day mortality significantly decreased by 32% in the hemoglobin group ≥ 7.2 g/dl (OR = 0.68, 95%CI 0.51–0.93, P = 0.0142). The non-linear relationship between admission hemoglobin level and 30-day mortality in sepsis was found. Hemoglobin supplementation might be beneficial for septic patients with hemoglobin level < 7.2 g/dl

Chylothorax and constrictive pericarditis in a woman due to generalized lymphatic anomaly: a case report

Abstract Background Generalized lymphatic anomaly (GLA) is characterized by diffuse or multicentric proliferation of dilated lymphatic vessels resembling common lymphatic malformations. Compared with soft tissue or bone involvement, thoracic involvement may be associated with a worse prognosis. Case presentation We reported a case of GLA with chylothorax and constrictive pericarditis in a 29-year-old woman. This patient exhibited remarkable features, including a continuously hemorrhagic chylothorax, constrictive pericarditis, and involvement of bone and neck lymph nodes. After attempting to manage her condition with conservative treatment, the patient underwent pericardial stripping surgery. Exploration revealed abundant hyperplasia of tubular tissue in the aortopulmonary window in both pleural cavities. Conclusions This case highlights the importance of maintaining the clinical suspicion of GLA during the follow-up of chylothorax patients. Aggressive pericardial surgery, which is important for both diagnosis and treatment, should be performed in patients with GLA with constrictive pericarditis

Research on High-Performance Reception Technology for 50 Gbit/s PON

【Objective】In order to meet the growing demand for ultra-gigabit, from gigabit to 10-gigabit, from The 5th Generation Fixed networks (F5G) to F5G-Advanced (F5.5G), 50 Gbit/s Passive Optical Network (PON) is considered to be an important part of F5.5G. This paper conducts a thorough study of 50 Gbit/s PON technology, reflecting the latest advancements in access networks.【Methods】This paper initially addresses the difficulties in achieving high sensitivity in 50 Gbit/s PON, and proposes a solution. 50 Gbit/s Non-Return-to-Zero (NRZ) signals pass through Avalanche Photodiode (APD) detectors to form macroscopic currents that can be detected under strong electric fields. These currents are then amplified by a Transresistance Amplifier (TIA) and converted into voltage outputs. After balancing, the Feed Forward Equalizer (FFE) and Decision Feedback Equalizer (DFE) of optical Digital Signal Processing (oDSP) chip are used to compensate the trailing phenomenon of pulse signal, and the influence of intersymbol interference is minimized by DFE. Then the paper analyses the key technologies such as APD, TIA and oDSP, comparing the reception performance between 25 Gbit/s and 50 Gbit/s APDs.【Results】Experimental test results show that the 25 Gbit/s APD maintains error-free signal reception at -8.48 dBm over 4 minutes, and reaches -26.61 dBm at a Bit Error Rate(BER) of 2.78e-2. The 50 Gbit/s APD exhibits no bit errors at -8.97 dBm and achieves -27.05 dBm at the same BER, with the second 50 Gbit/s APD set demonstrating similar results.【Conclusion】The 50 Gbit/s APD has superior receiving sensitivity and performance, making it highly suitable for 50 Gbit/s PON optical modules to help achieve high receiver sensitivity. The paper suggests a feasible approach for cost reduction in future implementations, focusing on the balance technology and APD

Accurate Determination of Interfacial Protein Secondary Structure by Combining Interfacial-Sensitive Amide I and Amide III Spectral Signals

Accurate determination of protein structures at the interface is essential to understand the nature of interfacial protein interactions, but it can only be done with a few, very limited experimental methods. Here, we demonstrate for the first time that sum frequency generation vibrational spectroscopy can unambiguously differentiate the interfacial protein secondary structures by combining surface-sensitive amide I and amide III spectral signals. This combination offers a powerful tool to directly distinguish random-coil (disordered) and α-helical structures in proteins. From a systematic study on the interactions between several antimicrobial peptides (including LKα14, mastoparan X, cecropin P1, melittin, and pardaxin) and lipid bilayers, it is found that the spectral profiles of the random-coil and α-helical structures are well separated in the amide III spectra, appearing below and above 1260 cm^–1, respectively. For the peptides with a straight backbone chain, the strength ratio for the peaks of the random-coil and α-helical structures shows a distinct linear relationship with the fraction of the disordered structure deduced from independent NMR experiments reported in the literature. It is revealed that increasing the fraction of negatively charged lipids can induce a conformational change of pardaxin from random-coil to α-helical structures. This experimental protocol can be employed for determining the interfacial protein secondary structures and dynamics in situ and in real time without extraneous labels

Phosphate Ions Promoting Association between Peptide and Modeling Cell Membrane Revealed by Sum Frequency Generation Vibrational Spectroscopy

Phosphate ion is one of the most important anions present in the intracellular and extracellular fluid. It can form strongly hydrogen-bonded and salt-bridged complexes with arginine and lysine to activate the voltage gated channel protein. A molecular-level insight into how the phosphate anions mediate the interaction between peptides and cell membrane is critical to understand membrane-bound peptide actions. In this study, sum frequency generation vibrational spectroscopy (SFG-VS) has been applied to characterize interactions between mastoparan (MP, a G-protein-activating peptide) and different charged lipid bilayers in situ. It is found that phosphate ions can greatly promote the association of MP with lipid bilayers and accelerate the conformation transition of membrane-bound MP from aggregation into α-helical structure. In phosphate buffer solution, MP can insert not only into negatively and neutrally charged lipid bilayers but also into positively charged lipid bilayers. In neutrally and negatively charged lipid bilayers, the tilt angle of α-helical structure becomes smaller with increasing buffer concentration, while MP adopts a multiple orientation distribution in the positively charged lipid bilayer. MP interacts with lipid bilayers in the salt solution environment most likely by formation of toroidal pores inside the bilayer matrix. Results from our studies will provide insight into the MP action mechanism and offer some ideas to deliver exogenous protein into the cytosol