1 research outputs found
Responses of <i>Phanerochaete chrysosporium</i> to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification
The white-rot fungus <i>Phanerochaete chrysosporium</i> has been widely used for the treatment of waste streams containing
heavy metals and toxic organic pollutants. The development of fungal-based
treatment technologies requires detailed knowledge of the relationship
between bulk water quality and the physiological responses of fungi.
A noninvasive microtest technique was used to quantify real-time changes
in proton, oxygen, and cadmium ion fluxes following the exposure of <i>P. chrysosporium</i> to environmental toxic (2,4-dichlorophenol
and cadmium). Significant changes in H<sup>+</sup> and O<sub>2</sub> flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1
mM cadmium. Cd<sup>2+</sup> flux decreased with time. Reactive oxygen
species formation and antioxidant levels increased after cadmium treatment.
Superoxide dismutase activity correlated well with malondialdehyde
levels (r<sup>2</sup> = 0.964) at low cadmium concentrations. However,
this correlation diminished and malondialdehyde levels significantly
increased at the highest cadmium concentration tested. Real-time microscale
signatures of H<sup>+</sup>, O<sub>2</sub>, and Cd<sup>2+</sup> fluxes
coupled with oxidative stress analysis can improve our understanding
of the physiological responses of <i>P. chrysosporium</i> to toxic pollutants and provide useful information for the development
of fungal-based technologies to improve the treatment of wastes cocontaminated
with heavy metals and organic pollutants