Effects of heme on the structure of the denatured state and folding kinetics of cytochrome b562

Heme-linked proteins, such as cytochromes, are popular subjects for protein folding studies. There is the underlying question of whether the heme affects the structure of the denatured state by cross-linking it and forming other interactions, which would perturb the folding pathway. We have studied wild-type and mutant cytochrome b562 from Escherichia coli, a 106 residue four-α-helical bundle. The holo protein apparently refolds with a half-life of 4 μs in its ferrous state. We have analysed the folding of the apo protein using continuous-flow fluorescence as well as stopped-flow fluorescence and CD. The apo protein folded much more slowly with a half-life of 270 μs that was unaffected by the presence of exogenous heme. We examined the nature of the denatured states of both holo and apo proteins by NMR methods over a range of concentrations of guanidine hydrochloride. The starting point for folding of the holo protein in concentrations of denaturant around the denaturation transition was a highly ordered native-like species with heme bound. Fully denatured holo protein at higher concentrations of denaturant consisted of denatured apo protein and free heme. Our results suggest that the very fast folding species of denatured holo protein is in a compact state, whereas the normal folding pathway from fully denatured holo protein consists of the slower folding of the apo protein followed by the binding of heme. These data should be considered in the analysis of folding of heme proteins.This work was supported by the BBSRC under the SBDA initiative, the EU TMR Life Sciences programme (contract ERBFMRX-CT-98-0230), the MRC and the NIH (grant GM056250 to H.R.). P.D.B. thanks the BBSRC for an Advanced Research Fellowshi

Effects of Heme on the Structure of the Denatured State and Folding Kinetics of Cytochrome b 562

transition was a highly ordered native-like species with heme bound. Fully denatured holo protein at higher concentrations of denaturant consisted of denatured apo protein and free heme. Our results suggest that the very fast folding species of denatured holo protein is in a compact state, whereas the normal folding pathway from fully denatured holo protein consists of the slower folding of the apo protein followed by the binding of heme. These data should be considered in the analysis of folding of heme proteins. q 2005 Published by Elsevier Ltd. Keywords: cytochrome b 562 ; heme; folding kinetics; continuous flow; NMR *Corresponding author Introduction Heme-linked proteins, especially cytochrome c, have been favourite targets for protein folding studies because of their ready availability, large spectral changes associated with the heme, and the exploitability of its redox state. But, WittungStafshede has reviewed the roles of cofactors in protein folding and has raised importan