18 research outputs found
Application of the support vector machine for processing the results of tin ores enrichment by the centrifugal concentration method
The relevance of the research is due to the acquisition of new knowledge about the features of the applicability of the support vector machine, related to machine learning tools, for solving problems of mathematical modeling of mining and processing equipment. The purpose of the research is a statistical analysis of the results of semi-industrial tests of the Knelson CVD technology on tin raw materials using the support vector machine method and the development of mathematical models suitable for further optimization of the technological parameters of the equipment. The objects of research were the products obtained as a result of the operation of hydro-cyclones, as well as the technological parameters of the operation of centrifugal concentrators. The work uses classical methods of mathematical statistics, the least squares method for constructing a linear regression model, the support vector machine implemented on the basis of the Scikit-learn library, as well as the method of verifying the resulting models based on the ShuffleSplit library. A general description of the process of testing the Knelson concentrator with continuous controlled unloading in relation to the enrichment of tin ores is presented. The results obtained were processed using the support vector machine. Regression models are obtained in the form of polynomials of the second degree and in the form of radial basis functions. A significant non-linearity is shown in the dependence between the content of the valuable component in the tailings and the values of the technological parameters of the apparatus
Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab
<p>Abstract</p> <p>Background</p> <p>Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have revolutionized the field of molecular biology. Indeed many standard molecular strategies, including molecular cloning, studies of DNA-protein interactions, and analysis of nucleic acid structures, would be virtually impossible without these versatile enzymes. The discovery of nucleases with unique properties has often served as the basis for the development of modern molecular biology methods. Thus, the search for novel nucleases with potentially exploitable functions remains an important scientific undertaking.</p> <p>Results</p> <p>Using degenerative primers and the rapid amplification of cDNA ends (RACE) procedure, we cloned the Duplex-Specific Nuclease (DSN) gene from the hepatopancreas of the Kamchatka crab and determined its full primary structure. We also developed an effective method for purifying functional DSN from the crab hepatopancreas. The isolated enzyme was highly thermostable, exhibited a broad pH optimum (5.5 – 7.5) and required divalent cations for activity, with manganese and cobalt being especially effective. The enzyme was highly specific, cleaving double-stranded DNA or DNA in DNA-RNA hybrids, but not single-stranded DNA or single- or double-stranded RNA. Moreover, only DNA duplexes containing at least 9 base pairs were effectively cleaved by DSN; shorter DNA duplexes were left intact.</p> <p>Conclusion</p> <p>We describe a new DSN from Kamchatka crab hepatopancreas, determining its primary structure and developing a preparative method for its purification. We found that DSN had unique substrate specificity, cleaving only DNA duplexes longer than 8 base pairs, or DNA in DNA-RNA hybrids. Interestingly, the DSN primary structure is homologous to well-known Serratia-like non-specific nucleases structures, but the properties of DSN are distinct. The unique substrate specificity of DSN should prove valuable in certain molecular biology applications.</p
Microscopic model of optical potential for testing the
The data on the 12,14Be + p elastic scattering cross sections at 700 Mev are compared with those obtained by solving the relativistic wave equation with the microscopic optical potentials calculated as folding of the NN amplitude of scattering with densities of these nuclei in the form of the symmetrized Fermi function with the fitted radius and diffuseness parameters, and also with the densities obtained in two microscopic models, based on the Generator Coordinate Method (GCM) and the other one – on the Variational Method of Calculations (VMC). For 12Be, above models turn out to be in a small disagreement with the data at "large" angles of scattering θ ≥ 9°, while for the 14Be one sees some inconsistence at smaller angles, too
Microscopic model of optical potential for testing the 12,14Be+p elastic scattering at 700 Mev
The data on the 12,14Be + p elastic scattering cross sections at 700 Mev are compared with those obtained by solving the relativistic wave equation with the microscopic optical potentials calculated as folding of the NN amplitude of scattering with densities of these nuclei in the form of the symmetrized Fermi function with the fitted radius and diffuseness parameters, and also with the densities obtained in two microscopic models, based on the Generator Coordinate Method (GCM) and the other one – on the Variational Method of Calculations (VMC). For 12Be, above models turn out to be in a small disagreement with the data at "large" angles of scattering θ ≥ 9°, while for the 14Be one sees some inconsistence at smaller angles, too
Comparative analysis of the pion-nucleus scattering within the microscopic Folding and the local Kisslinger type potentials
Elastic scattering cross sections are calculated and compared to the data of π± + 28Si and 40Ca at energies 130, 180, and 230 MeV by using the both microscopic optical potentials (OP), the high-energy folding and the local Kisslinger type potentials. In the folding OP, we use the known nuclear density distributions while parameters of the elementary πN-scattering amplitude are fitted to the data with the aim to estimate the inmedium effect on pions scattered on bound nucleons. As to the local modified Kisslinger OP its parameters are known from the earlier studies. The cross sections for both OPs are calculated by solving the Klein-Gordon wave equation, and thus the relativistic and distortion effects on the process are accounted for exactly. A fairly well agreement with experimental data was obtained and the decisive role of a surface region of potentials was established where both OPs occur in close coincidence
Comparative analysis of the pion-nucleus scattering within the microscopic Folding and the local Kisslinger type potentials
Elastic scattering cross sections are calculated and compared to the data of π± + 28Si and 40Ca at energies 130, 180, and 230 MeV by using the both microscopic optical potentials (OP), the high-energy folding and the local Kisslinger type potentials. In the folding OP, we use the known nuclear density distributions while parameters of the elementary πN-scattering amplitude are fitted to the data with the aim to estimate the inmedium effect on pions scattered on bound nucleons. As to the local modified Kisslinger OP its parameters are known from the earlier studies. The cross sections for both OPs are calculated by solving the Klein-Gordon wave equation, and thus the relativistic and distortion effects on the process are accounted for exactly. A fairly well agreement with experimental data was obtained and the decisive role of a surface region of potentials was established where both OPs occur in close coincidence
Digital tools for seaports geo-information support while climate change and Covid-19 pandemic
In article, there are presented digital tools development results for geo-information support to seaports activity within Industry 4.0 period, when while climate change and covid-19 pandemic. In study, there are used methods of data bases constructing, web-technologies and virtual reality tools. Also, there are used Foresight technologies, theory of decision making under uncertainties and risk management. As digital tools, there is proposed to use digital online platforms, which integrate heterogeneous hardware and software resources with the use of web-technologies in distributed networks and wide application of cloud services. In study, there are used data bases and tools of geo-information digital online platform EOS, including its Land Viewer product. As study result, there is demonstrated usage of geo-information support system for Russian seaport Ust-Luga. The research results presented in this article has significant scientific novelty and can be useful for private investors, public environmental organizations of the civil sector and state environmental control bodies
Model of baddeleyite recovery from dump products of an apatite-baddeleyite processing plant using a CVD6 concentrator
The paper is devoted to developing a model of baddeleyite recovery from dump products of an apatite-baddeleyite processing plant using centrifugal concentrators. The relevance of the work arises from the acquisition of new knowledge on the optimization of technological parameters of centrifugal concentrators using Knelson CVD (continuous variable discharge) technology – in particular, setting the frequency of valve opening and the duration of valves remaining open. The purpose of the research was to assess the applicability of CVD technology in the treatment of various dump products of the processing plant and to build a model of dependencies between the concentrate and tailings yields and the adjustable parameters, which will allow to perform preliminary calculations of the efficiency of implementing this technology at processing plants. The research objects are middling and main separation tailings of the coarse-grained stream and combined product of main and recleaner separation tailings of the fine-grained stream. The study uses general methods of mathematical statistics: methods of regression analysis, aimed at building statistically significant models, describing dependence of a particular variable on a set of regressors; group method of data handling, the main idea of which is to build a set of models of a given class and choose the optimal one among them. Authors proposed an algorithm for processing experiment results based on classical regression analysis and formulated an original criterion for model selection. Models of dependencies between the concentrate and tailings yields and the adjustable parameters were built, which allowed to establish a relationship between the concentrate yield and the valve opening time, as well as a relationship between the tailings yield and the G-force of the installation
MECHATRONICS TECHNOLOGICAL SYSTEMS INTELLECTUAL MANAGEMENT PROSPECTS AND RESULTS
DSTU outlook on research in mechatronics management intellectual systems creation is considered. Agricultural harvesting machine control problems are considered as examples
A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas
We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for nonperfectly matched duplexes of the same length. Thus, DSN differentiates between one-nucleotide variations in DNA. We developed a novel assay for single nucleotide polymorphism (SNP) detection based on this unique property, termed “duplex-specific nuclease preference” (DSNP). In this innovative assay, the DNA region containing the SNP site is amplified and the PCR product mixed with signal probes (FRET-labeled short sequence-specific oligonucleotides) and DSN. During incubation, only perfectly matched duplexes between the DNA template and signal probe are cleaved by DSN to generate sequence-specific fluorescence. The use of FRET-labeled signal probes coupled with the specificity of DSN presents a simple and efficient method for detecting SNPs. We have employed the DSNP assay for the typing of SNPs in methyltetrahydrofolate reductase, prothrombin and p53 genes on homozygous and heterozygous genomic DNA. [Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to GenBank/EMBL/Date Bank under accession nos. AF520591. The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: N.K. Yankovsky, A.V. Polyakov, and G.N. Rudenskaya.