1 research outputs found
NMR and Molecular Recognition of N‑Glycans: Remote Modifications of the Saccharide Chain Modulate Binding Features
Glycans
play a key role as recognition elements in the communication
of cells and other organisms. Thus, the analysis of carbohydrate–protein
interactions has gained significant importance. In particular, nuclear
magnetic resonance (NMR) techniques are considered powerful tools
to detect relevant features in the interaction between sugars and
their natural receptors. Here, we present the results obtained in
the study on the molecular recognition of different mannose-containing
glycans by <i>Pisum sativum</i> agglutinin. NMR experiments
supported by Corcema-ST analysis, isothermal titration calorimetry
(ITC) experiments, and molecular dynamics (MD) protocols have been
successfully applied to unmask important binding features and especially
to determine how a remote branching substituent significantly alters
the binding mode of the sugar entity. These results highlight the
key influence of common structural modifications in natural glycans
on molecular recognition processes and underscore their importance
for the development of biomedical applications