牛山英治が編纂した山岡鉄舟の伝記について

Table S8. Comparison of GD in different studies. MICN is an abbreviation of Modified introduction in China; TS is an abbreviation of Tropical/Subtropical; SS is an abbreviation of Stiff Stalk; NSS is an abbreviation of non-Stiff Stalk; HZS is an abbreviation of Huangzaosi. (XLSX 11 kb

Differentially expressed siRNAs, differentially expressed genes, and differentially methylated genes in embryonic calli.

<p>(A-C) Venn diagrams displaying the numbers of differentially expressed siRNAs and differentially methylated target genes in the 3 stages. (D-F) Venn diagrams displaying numbers of differentially expressed and differentially methylated target genes in the 3 stages. The <i>P</i>-<i>values</i> of the correlations are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180567#pone.0180567.s003" target="_blank">S2 Table</a>.</p

Expression patterns and gene functions of target genes.

<p>(A-C) The patterns of target genes and significantly enriched (<i>P</i> < 0.05) GO terms in clusters 1, 2 and 3.</p

siRNAs identified in the process of callus formation.

<p>(A) The number of siRNAs identified in the 4 samples. (B) The number of siRNAs of different lengths. (C) Venn diagram of the differentially expressed siRNAs identified in the 4 samples.</p

Expression of 8 DE-siRNAs and their target genes in 4 samples.

<p>Expression is shown as log₂ (FC) as calculated in the comparison of the samples from Stages I, II and III to the CK sample. “Deep-seq” and “RT-qPCR” represent the relative expression levels of the siRNAs and target genes derived from the deep sequencing and real-time qPCR, respectively. “cor bet siRNA & target” represent the correlation between the expression level of the siRNA and its target genes derived from the deep sequencing.</p

<i>HB25</i> was cleaved by zm-siR004119-2.

<p>(A) The cleavage site of <i>HB25</i> was in the 5’ UTR at 37–38 bp. (B) The relative expression levels of zm-siR004119-2 and <i>HB25</i> during callus formation. Expression is expressed as log₂ (FC) as calculated in the comparison of the samples from Stage I, II and III to that of CK. (C) The relative expression levels of zm-siR004119-2 and <i>HB25</i> calli induced from the 18R, IBM107, IBM127 and IBM164 lines. Expression level is calculated for the comparison of the samples from IBM107, IBM127 and IBM164 to that from 18R, and the results are shown as the means ± SD (n = 3). *P < 0.05 (Student’s t-test) for the differences between 18R and IBM107, 18R and IBM127, or 18R and IBM164.</p

Associations among siRNA up-regulation, DNA hyper-methylation and transcriptional down-regulation of GRMZM2G013465 in CK and samples from the 3 stages.

<p>(A) IGV track of the MeDIP data in GRMZM2G013465. Turquoise, blue, pink and yellow: MeDIP-seq tracks of the samples from the CK group and samples from Stages I, II and III, respectively; red outlines the hyper-methylated region of the gene of interest. The colored rectangles represent the differentially expressed siRNAs targeting the 3’ UTR of GRMZM2G013465. (B) Fold changes of these differentially expressed siRNAs during callus induction. (C) Fold changes of GRMZM2G013465 in the 3 samples calculated from the deep sequencing and real-time qPCR data. (D) The expression levels of GRMZM2G013465 in different types of calli induced from the 18R, IBM107, IBM127 and IBM164 lines. Expression is calculated for the comparison of samples from IBM107, IBM127 and IBM164 to that from 18R, and the results are shown as the means ± SD (n = 3). *P < 0.05 and ** P < 0.01 (Student’s t-test) for differences between 18R and IBM107, 18R and IBM127, or 18R and IBM164.</p

Additional file 13: of Characterizing the population structure and genetic diversity of maize breeding germplasm in Southwest China using genome-wide SNP markers

Table S7. Similarity ratio of 30 inbred lines most similar to S37 for 10 chromosomes. (DOCX 18 kb

Large-Area Conductive MOF Ultrathin Film Controllably Integrating Dinuclear-Metal Sites and Photosensitizers to Boost Photocatalytic CO₂ Reduction with H₂O as an Electron Donor

Owing to the electrical conductivity and periodic porosity, conductive metal–organic framework (cMOF) ultrathin films open new perspectives to photocatalysis. The space-selective assembly of catalytic sites and photosensitizers in/on cMOF is favorable for promoting the separation of photogenerated carriers and mass transfer. However, the controllable integration of functional units into the cMOF film is rarely reported. Herein, via the synergistic effect of steric hindrance and an electrostatic-driven strategy, the dinuclear-metal molecular catalysts (DMC) and perovskite (PVK) quantum dot photosensitizers were immobilized into channels and onto the surface of cMOF ultrathin films, respectively, affording [DMC@cMOF]-PVK film photocatalysts. In this unique heterostructure, cMOF not only facilitated the charge transfer from PVK to DMC but also guaranteed mass transfer. Using H2O as an electron donor, [DMC@cMOF]-PVK realized a 133.36 μmol·g–1·h–1 CO yield in photocatalytic CO2 reduction, much higher than PVK and DMC-PVK. Owing to the excellent light transmission of films, multilayers of [DMC@cMOF]-PVK were integrated to increase the CO yield per unit area, and the 10-layer device realized a 1115.92 μmol·m–2 CO yield in 4 h, which was 8-fold higher than that of powder counterpart. This work not only lightens the development of cMOF-based composite films but also paves a novel avenue for an ultrathin film photocatalyst

Large-Area Conductive MOF Ultrathin Film Controllably Integrating Dinuclear-Metal Sites and Photosensitizers to Boost Photocatalytic CO₂ Reduction with H₂O as an Electron Donor

Owing to the electrical conductivity and periodic porosity, conductive metal–organic framework (cMOF) ultrathin films open new perspectives to photocatalysis. The space-selective assembly of catalytic sites and photosensitizers in/on cMOF is favorable for promoting the separation of photogenerated carriers and mass transfer. However, the controllable integration of functional units into the cMOF film is rarely reported. Herein, via the synergistic effect of steric hindrance and an electrostatic-driven strategy, the dinuclear-metal molecular catalysts (DMC) and perovskite (PVK) quantum dot photosensitizers were immobilized into channels and onto the surface of cMOF ultrathin films, respectively, affording [DMC@cMOF]-PVK film photocatalysts. In this unique heterostructure, cMOF not only facilitated the charge transfer from PVK to DMC but also guaranteed mass transfer. Using H2O as an electron donor, [DMC@cMOF]-PVK realized a 133.36 μmol·g–1·h–1 CO yield in photocatalytic CO2 reduction, much higher than PVK and DMC-PVK. Owing to the excellent light transmission of films, multilayers of [DMC@cMOF]-PVK were integrated to increase the CO yield per unit area, and the 10-layer device realized a 1115.92 μmol·m–2 CO yield in 4 h, which was 8-fold higher than that of powder counterpart. This work not only lightens the development of cMOF-based composite films but also paves a novel avenue for an ultrathin film photocatalyst