1 research outputs found
Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms
Autoantibodies
raised against tumor-associated antigens have shown
high promise as clinical biomarkers for reliable diagnosis, prognosis,
and therapy monitoring of cancer. An electrochemical disposable biosensor
for the specific and sensitive determination of p53-specific autoantibodies
has been developed for the first time in this work. This biosensor
involves the use of magnetic microcarriers (MBs) modified with covalently
immobilized HaloTag fusion p53 protein as solid supports for the selective
capture of specific autoantibodies. After magnetic capture of the
modified MBs onto screen-printed carbon working electrodes, the amperometric
signal using the system hydroquinone/H<sub>2</sub>O<sub>2</sub> was
related to the levels of p53-autoantibodies in the sample. The biosensor
was applied for the analysis of sera from 24 patients with high-risk
of developing colorectal cancer and 6 from patients already diagnosed
with colorectal (4) and ovarian (2) cancer. The developed biosensor
was able to determine p53 autoantibodies with a sensitivity higher
than that of a commercial standard ELISA using a just-in-time produced
protein in a simpler protocol with less sample volume and easily miniaturized
and cost-effective instrumentation