Anti-angiogenic drug loaded liposomes: Nanotherapy for early atherosclerotic lesions in mice - Fig 4

<p>(A) Fluorescence imaging of harvested aortas. The arrows point the patchy areas where plaques are located. (B) Harvested aortas stained with Oil Red O.</p

Chemical structure of fumagillin.

<p>Chemical structure of fumagillin.</p

Chromatographic conditions for the analysis of fumagillin content by HPLC-UV.

<p>Chromatographic conditions for the analysis of fumagillin content by HPLC-UV.</p

Percent of aortic arch plaque obtained after treatment with angiogenesis inhibitors (LF and ILF) in mice.

<p>Plain liposomes control <b>L</b> (x), liposomes loaded with fumagillin <b>LF</b> (○), and immunoliposomes with fumagillin <b>ILF</b> (Δ) animals were treated for 5 weeks as previously described. Red line centered at each group represents median plaque area of aortic arch lesions measured in a cohort (n = 6).</p

Experimental plan designed to test the action of liposomes loaded with the anti-angiogenic agent fumagillin in early atherosclerotic lesions.

<p>Experimental plan designed to test the action of liposomes loaded with the anti-angiogenic agent fumagillin in early atherosclerotic lesions.</p

Percentage of lesion area relative to the area of the aortic arch.

<p>Percentage of lesion area relative to the area of the aortic arch.</p

Cross-sectional images of the aortic arch on <i>in vivo</i> T1-weighted MRI of mice treated with L and LF liposomes and obtained 24 hours after final injection.

<p>Cross-sectional images of the aortic arch on <i>in vivo</i> T1-weighted MRI of mice treated with L and LF liposomes and obtained 24 hours after final injection.</p

Representative chromatogram of the fumagillin sample obtained after liposome extraction.

<p>Fumagillin spectra presented two maximum peaks of absorbance at 335.5 and 351.1 nm. We used a wavelength of 351nm for the detection of fumagillin to avoid interferences from other liposome components. The retention time obtained was 5.35 ± 0.05 minutes (mean ± SD; n = 10).</p

hAAT gene translation index in liver tissue.

<p>Fig 3 represents the gene translation index per cell, calculated per ELISA, after both the endovascular and surgery closed perfusion procedures. Translation in each liver lobe is represented (mean ± SD). First letter: R = right, L = left; Second letter: M = medial, L = lateral; Number: 1 = upper, 2 = lower. Statistical analysis: linear mixed model with pig as random factor, hAAT protein as response variable, and group, liver lobe and their interaction as explicative variables.</p

hAAT Immunohistochemistry.

<p>Non-transfected pig liver hAAT staining (a; 40x) versus transfection employing endovascular closed catheterization (b; 40x). hAAT protein staining in liver sections of transfected mice is shown (c; 40x); human liver hAAT immunostaining can be observed (d; 40x). Black arrows indicate representative specific immunohistochemical reaction against hAAT protein in pig samples. Tissue was counterstained with hematoxylin.</p