Representative immunofluorescence of retinal sections showing the expression pattern of GLAST following ischemia.

<p>Glast immunoreactivity in the ischemic retina after 150 min of reperfusion (ISCH/REP) shows no evident changes in the expression and distribution of the glial transporter compared to the contralateral non-ischemic retina (CTL). (GCL) ganglion cell layer (IPL), inner plexiform layer, (INL) inner nuclear layer, (OPL) outer plexiform layer, (ONL) outer nuclear layer.</p

Modulation of [³H]-D-Aspartate release and uptake in synaptosomes from ischemic retina.

<p>(A) <i>Ischemia reduced [³H]-D-Asp uptake in highly pure nerve ending.</i> [³H]-D-Asp uptake into synaptosomes was measured in animals subjected to retinal ischemia for 50 min in the right eye and reperfused for 150 min (dashed line). The contralateral eye (solid line) was used as control. Data are expressed as mean ± S.E.M. (n = 3 or 4 animals per group). (B) <i>[³H]-D-Asp release in retinal synaptosomes.</i> Animals were subjected to retinal ischemia for 50 min in the right eye and reperfusion was allowed for 150 min. Release was measured in the ischemic (white histograms), contralateral (grey) and naïve retinas (black). All results are expressed as percentage of the corresponding KCl-evoked release (15 mM). Omission of Ca²⁺ produces a reduction of [³H]-D-Asp release of about 40% in all groups, while DL-TBOA reduces by about 60% the release in naïve and contralateral samples, but not in the ischemic retina. *p<0.05 vs control.</p

Effects of retinal ischemia/reperfusion on the expression and distribution of glutamate transporter GLT-1.

<p>GLT-1 immunoreactivity increases in the retina of rats subjected to 50 min of ischemia followed by 150 min of reperfusion (ISCH/REP) as compared to the control, non-ischemic retina (CTL). An increased number of GLT-1 positive cell bodies is evident in the ONL and INL of retinas subjected to ischemia (ISCH/REP). (GCL) ganglion cell layer (IPL), inner plexiform layer, (INL) inner nuclear layer, (OPL) outer plexiform layer, (ONL) outer nuclear layer.</p

Native and bi-dimensional western blotting analysis of GLT-1.

<p>(A) GLT-1 immunoreactivity is increased under native conditions in the ischemic retina (I/R) after 150 min of reperfusion when compared to the non-ischemic contralateral eye (C). Histogram shows the results of the densitometric analysis of autoradiographic bands normalized to the value of loading control. Each value is the mean ± S.E.M. of three experiments. (B) A representative Native/SDS-PAGE image showing the appearance in the second dimension (SDS-PAGE) of a 38 kDa band up-regulated in the retina subjected to 50 min of ischemia and 150 min reperfusion (ISCH/REP) as compared to the contralateral non-ischemic retina (CTL).</p