Isolation of an alveolar septum by laser-assisted microdissection and manipulation from a hemalaun stained frozen lung section (magnification 200×)

<p><b>Copyright information:</b></p><p>Taken from "Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS"</p><p>BMC Biotechnology 2004;4():30-30.</p><p>Published online 3 Dec 2004</p><p>PMCID:PMC539305.</p><p>Copyright © 2004 Kwapiszewska et al; licensee BioMed Central Ltd.</p> A) Alveolar septum is selected for isolation. B) Laser photolysis is used to disconnect the cells from adjacent ones. C) Septum cells adhere tightly to the approximated sterile needle and can be transferred into a reaction tube

Antibody details.

<p>Antibody details.</p

Immunohistochemical analysis and quantification of CD11c-positive cells in human lung cancer.

<p>Human lung cancer tissue array was stained with CD11c antibody to detect dendritic cells. (A) Quantification of CD11c⁺ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD11c⁺ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD11c-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD11c antibody based on their pathology. Scale bar = 25 μm.</p

Additional file 1: of Increased S100A4 expression in the vasculature of human COPD lungs and murine model of smoke-induced emphysema

Supplemental information about data collecion, material and methods. (PDF 567 kb

Immunohistochemical analysis and quantification of CD4-positive T lymphocytes in human lung cancer.

<p>Human lung cancer tissue array was stained with CD4 antibody to detect T helper cells. (A) Quantification of CD4⁺ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD4⁺ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD4-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD4 antibody based on their pathology. Scale bar = 25 μm.</p

Morphological analysis of human lung specimens.

<p>Representative images of human lung sections stained with hematoxylin and eosin based on their pathology. (A) Healthy donor, (B) squamous cell carcinoma, (C) adenocarcinoma, (D) adenosquamous carcinoma, (E) small cell carcinoma, (F) papillary adenocarcinoma, (G) metastatic adenocarcinoma, and (H) bronchioloalveolar carcinoma. Scale bar = 250 μm.</p

Immunohistochemical analysis and quantification of CD3-positive T lymphocytes in human lung cancer.

<p>Human lung cancer tissue array was stained with CD3 antibody to detect T lymphocytes. (A) Quantification of CD3⁺ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD3⁺ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD3-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD3 antibody based on their pathology. Scale bar = 25 μm.</p

Immunohistochemical analysis and quantification of PD1–positive cells in human lung cancer.

<p>Human lung cancer tissue array was stained with PD1 antibody to detect activated T cells, B cells, myeloid cells, and a subset of thymocytes. (A) Quantification of PD1⁺ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of PD1⁺ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as PD1–positive cells per 1000 cells. (F) Representative images of human lung sections stained with PD1 antibody based on their pathology. Scale bar = 25 μm.</p

Immunohistochemical analysis and quantification of CD8-positive T lymphocytes in human lung cancer.

<p>Human lung cancer tissue array was stained with CD8 antibody to detect cytotoxic T lymphocytes. (A) Quantification of CD8⁺ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD8⁺ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD8-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD8 antibody based on their pathology. Scale bar = 25 μm.</p

β-catenin dependent transcription mediates upregulation of <i>Fgfr2b</i> expression, which is inhibited in PR/8-infected, but not in non-infected lung epithelial cells.

<p>(A) EpCam⁺ lung epithelial cells derived from <i>Rosa26</i>^{<i>ERTCre/ERTCre</i>}<i>;Ctnnb1</i>^{<i>flox/flox</i>} mice were grown to confluency and treated with tamoxifen or DMSO control prior to infection with PR/8 (MOI = 0.1; 24h). mRNA expression of β-catenin (<i>Ctnnb1</i>) (left) or of <i>Fgfr2b</i> (right) was quantified and normalized to values of DMSO-treated control. (B) Wt distal lung epithelial cells in confluent culture were PR/8-infected (MOI 0.1) and treated with an activator (LiCl) or inhibitor (XAV939) of β-catenin signaling. Expression of the viral M segment was quantified at 16 h pi and normalized to LiCl-treated cultures (left). The right plot shows representative photomicrographs of these cultures stained for IV nucleoprotein (NP) after 6 h of PR/8 infection. (C) Wt mice were infected with PR/8 for 7d and infected (IV hemagglutinin⁺, HA⁺) vs. non-infected (HA-) EpCam⁺ cells were flow-sorted. Expression of the β-catenin-dependent transcripts <i>Axin2</i>, <i>Fgfr2b</i>, and <i>Ccnd1</i> was quantified in HA- cells and normalized to values from HA⁺ cells. All bar graphs represent means ± SD of n = 3–4 independent experiments; * <i>p</i><0.05; **<i>p</i><0.01; Tam, tamoxifen.</p