SAMARAS OF AUSTROPLENCKIA POPULNEA (CELASTRACEAE): NEW CONSTITUENTS AND EFFECT OF EXTRACTS AND FRIEDELIN ON GERMINATION OF BIDENS PILOSA (ASTERACEAE)

Objective: Evaluation of the impact of extracts and constituents from samaras of Austroplenckia populnea on percentage of seed germination (%SG), germination speed index (GSI), length of rootlets (LR), seedling length (SL), and on dry mass (DM) of Bidens pilosa L weed.Methods: The  powder  of  samaras  was  extracted  with  organic  solvents  providing  the  hexane  (SAPEH),  chloroform  (SAPEC),  ethyl  acetate  (SAPEAE) and ethanol  (SAPEE)  extracts. The terpene 1 was isolated from SAPEH by means of column and thin layer chromatography and identified through NMR spectroscopy. Each extract and 1 were subjected to growth inhibition assays evaluating the following parameters: %SG, GSI, LR, SL and DM, with five repetitions.Results: The compounds Friedelin (1), 7-hydroxy-clerodan-3-en-16,15:18,20-diolide (2), 3,5,7,4'-tetrahydroxy-6-methoxy-8-prenylflavanone (3), tetradecanamide (4), and 4-hydroxy-1,6,15-acetyloxy-8,9-benzoyloxy-agarofurane (5) were isolated from hexane extract of samaras of A. populnea and identified by spectroscopic data. The compounds 2, 3 and 5 were not previously described as being chemical constituents from Celastraceae family. In addition, the novel compounds 3 and 5 were described here for the first time. Substantial effect on the germination of B. pilosa L. (picão-preto) was observed after treatment of seeds with nonpolar extracts from Samaras of A. populnea. Friedelin inhibited the seed germination in the tested concentrations showing toxic properties against picão-preto.Conclusion: The germination inhibition of seeds was higher using nonpolar extracts than polar extract. Friedelin inhibited the seed germination in the tested concentrations showing toxic properties against B. pilosa.Â

CYTOTOXICITY, ANTI-POLIOVIRUS ACTIVITY AND IN SILICO BIOLOGICAL EVALUATION OF CONSTITUENTS FROM MAYTENUS GONOCLADA (CELASTRACEAE)

Objective: The in silico free access web tools PASS online and ChemMapper were used to predict potential biological activities of compounds 1 to 8 isolated from Maytenus gonoclada (Celastraceae). The constituents 4'-O-methylepigalocatequin (6), tingenone (7) and proanthocyanidin A (8), and ethanolic extracts were subjected to in vitro cytotoxicity using VERO cells and anti-Poliovirus assays. Methods: QSAR and molecular superposition, correlating the average number of pharmacophores were used in the prediction studies. Cellular line VERO ATCC CCL-81 was used to determine anti-Poliovirus effect, observed by colorimetric (MTT) method. The annexing V/propidium iodide assay was used to determine the occurrence of apoptosis in the cytotoxicity assays. Results: The experimental results found for constituents 6-8 were in accordance with observed data obtained through PASS online and ChemMapper simulation. Conclusion: Compound 7 showed higher cytotoxic and apoptosis induction properties, and 6 and 8 presented anti-Poliovirus activity

Bioactivity of extracts from Alpinia zerumbet against sinusitis-causing bacterial pathogens

Made available in DSpace on 2017-06-01T19:35:42Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) 3.pdf: 180440 bytes, checksum: 005d8406177b2a827c7674f0e9caa588 (MD5) Previous issue date: 5Universidade de Itaúna. Faculdade de Farmácia. Minas Gerais, MG, Brasil.Universidade de Itaúna. Faculdade de Farmácia. Minas Gerais, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Minas Gerais, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Minas Gerais, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Minas Gerais, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Química. Minas Gerais, MG, Brasil.Universidade de Itaúna. Faculdade de Farmácia. Minas Gerais, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Química. Minas Gerais, MG, Brasil.Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. tem sido indicada na medicina popular brasileira para tratar reumatismo, dor de cabeça e sinusite. Para avaliar o efeito de extratos e frações de flores de Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm., a fim de validar cientificamente o uso da planta para o tratamento de sinusite, foi proposto este estudo. Os extratos e frações foram preparados e analisados por ensaios fitoquímicos. A atividade antibacteriana contra 12 patógenos bacterianos associados com a sinusite foi avaliada empregando o método de diluição em ágar. A fração hexânica apresentou o mais amplo espectro de atividade, inibindo 10 das 12 bactérias testadas, especialmente Porphyromonas gingivalis, Fusobacterium nucleatum e Fusobacterium necrophorum. O menor valor de MIC foi observado para a fração em acetato de etila contra Streptococcus pneumoniae (32 µgµL-1 ). Todos os extratos e frações testadas apresentaram atividade contra Prevotella intermedia. Por outro lado, nenhum extrato ou fração exibiu efeito antibacteriano contra Streptococcus agalactiae. A solução usada na medicina popular brasileira mostrou atividade inibitória contra os microorganismos isolados a partir de pacientes com sinusite crônica e aguda. Este estudo demonstra promissora atividade antibacteriana de Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. contra bactérias responsáveis pela sinusite aguda e crônica e valida o seu uso pela primeira vez.Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. is indicated in Brazilian traditional medicine to treat rheumatism, headache and sinusitis. To evaluate the effect of extracts and fractions of flowers from Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. in order to provide a scientific rationale for the use of the plant for the treatment of sinusitis, this study was realized. The extracts and fractions were prepared and analyzed by phytochemical investigation. Antibacterial activity against 12 bacterial pathogens associated with sinusitis was evaluated by employing an agar dilution method. The hexane fraction showed the broader activity spectrum inhibiting 10 out of the 12 tested bacteria specially Porphyromonas gingivalis, Fusobacterium nucleatum, and Fusobacterium necrophorum. The lower MIC value was observed for the ethyl acetate fraction against Streptococcus pneumoniae (32 µgµL-1). All extracts and fractions tested expressed activity against Prevotella intermedia. On the other hand, none of them exhibited antibacterial effect against Streptococcus agalactiae. The solution used in Brazilian traditional medicine showed inhibitory activity against microorganisms isolated from acute and chronic sinusitis patients. This study demonstrates the promising antibacterial activity of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. against bacteria responsible for acute and chronic sinusitis and validates its use for the first time

Evaluation of antimicrobial activity and toxic potential of extracts and triterpenes isolated from Maytenus imbricata

The phytochemical study of hexane/ethyl ether (1:1) extract of the roots of M. imbricata, Celastraceae, resulted in the isolation and characterization of six known triterpenes: 11α-hydroxylup-20(29)-en-3-one, previously isolated from this species besides, 3β,11α-di-hydroxylup-20(29)-ene, 3,7-dioxofriedelane, 3-oxo-29-hydroxyfriedelane, tingenone and 6-oxo-tingenol. The chemical structures of these triterpenes were established by spectrometric data (IR, ¹H and 13C NMR) and through comparison with literature data. The hexane/ethyl ether (1:1), ethyl acetate and methanol extracts, and 11α-hydroxylup-20(29)-en-3-one, tingenone and 6-oxo-tingenol, showed antimicrobial properties on in vitro assays. All extracts and triterpenes, except 3β,11α-di-hydroxylup-20(29)-ene, presented toxicity demonstrated by the larvicidal effect test using Artemia salina