Production of IFN-γ by CD4⁺ T cells in the first week after 17DD and 17D/ SIV Gag recombinants immunization in mice.

<p>Percentages of CD4⁺ IFN- γ⁺ cells in draining lymph nodes and spleens of mice following 17 DD – (Lymph node-panel A; Spleen- panel D), 17D SIV Gag _45-269 (Lymph node-panel B; Spleen- panel E) and 17D Δ IRES (Lymph node- panel C, Spleen- panel F) immunization in C57BL/6 and BALB/c mice. Statistical analysis was performed by Two- Way ANOVA with Bonferroni post-test. * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001. The means are representative of three independent experiments. N= 3 animals/ experimental point.</p

Functional profile of CD8⁺ T cell responses in mice immunized with 17DD and 17D/ SIV Gag recombinants.

<p>ICCS for YF-specific and Gag-specific CD8⁺ T-cells at day 14 after immunization on secretion of IFN-γ and/or IL-2 following stimuli with antigen (whole inactivated YF 17DD virus -panels A, C and E and peptide mixtures spanning amino acids 1–291 of Gag -panels B, D and F). Bar graphs indicate the mean frequency of CD8⁺ splenocytes specific to YF and Gag capable of producing IFN-γ (black), IL-2 (white) or both (grey). Error bars represent the standard error of the mean. Differences between cytokine + cells were statistically significant between BALB/c and C57BL/6 mice (* p<0.05, **p<0.01). The means are representative of three independent experiments. N= 4 animals/ experiment.</p

Production of IFN-γ by CD8⁺ T cells in the first week after 17DD and 17D/ SIV Gag recombinants immunization in mice.

<p>Percentages of CD8⁺ IFN- γ⁺ cells in draining lymph nodes and spleens of mice following 17 DD – (Lymph node-panel A; Spleen- panel D), 17D SIV Gag _45-269 (Lymph node-panel B; Spleen- panel E) and 17D Δ IRES (Lymph node- panel C, Spleen- panel F) immunization in C57BL/6 and BALB/c mice. Statistical analysis was performed by Two- Way ANOVA with Bonferroni post-test. * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001. The means are representative of three independent experiments. N= 3 animals/ experimental point.</p

Production of IFN-γ by γδ T cells in the first week after 17DD and 17D/ SIV Gag recombinants immunization in mice.

<p>Percentages of γδ⁺ T cells+ IFN- γ⁺ cells in draining lymph nodes and spleens of mice following 17 DD – (Lymph node-panel A; Spleen- panel D), 17D SIV Gag _45-269 (Lymph node-panel B; Spleen- panel E) and 17D Δ IRES (Lymph node- panel C, Spleen- panel F) immunization in C57BL/6 and BALB/c mice. Statistical analysis was performed by Two- Way ANOVA with Bonferroni post-test. * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001. The means are representative of three independent experiments. N= 3 animals/ experimental point.</p

Functional profile of CD4⁺ T cell responses in mice immunized with 17DD and 17D/ SIV Gag recombinants.

<p>ICCS for YF-specific and Gag-specific CD4⁺ T-cells at day 14 after immunization on secretion of IFN-γ and/or IL-2 following stimuli with antigen (whole inactivated YF 17DD virus -panels A, C and E and peptide mixtures spanning amino acids 1–291 of Gag -panels B, D and F). Bar graphs indicate the mean frequency of CD4⁺ splenocytes specific to YF and Gag capable of producing IFN-γ (black), IL-2 (white) or both (grey). Error bars represent the standard error of the mean. Differences between cytokine + cells were statistically significant between BALB/c and C57BL/6 mice (* p<0.05, **p<0.01). The means are representative of three independent experiments. N= 4 animals/ experiment.</p

Magnitude of YF and Gag-specific CD8⁺ T-cell responses in animals vaccinated with YF 17DD and YF 17D recombinant viruses.

<div><p>Groups of mice (C57BL/6 strain and BALB/c strain) were immunized twice 15 days apart with vehicle medium (mock), YF vaccine (17 DD), recombinant 17 D SIV-Gag _45-269 or recombinant 17 D Δ IRES virus. Spleen cells of each mouse were recovered 14 days after second immunization and cultured with 2 μg/mL of YF CD8 specific peptides (Panel A-C) or 5 mM of SIV Gag peptide pool (Panel D-F). Results are representative of three independent experiments and are normalized to non-stimulated cells control. Bars represent the medians. Statistics were done by Mann-Whitney T test. </p> <p>* <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001.</p></div

Correlations between neutralizing antibodies and IgG2a levels in sera of mice immunized with YF 17DD and YF 17D recombinant viruses.

<p>Neutralizing antibodies levels and IgG2a O.Ds in mouse sera following YF 17 DD (panels A and B), YF 17D SIV Gag _45-269 (panels C and D) and YF 17D Δ IRES (panels E and F) virus immunization in C57BL/6 and BALB/c mice. The analysis was performed by Pearson’s correlation method.</p

Humoral immune responses in animals vaccinated with YF 17DD and YF 17D recombinant viruses.

<p>Neutralizing antibodies levels, Total YF- IgG levels and IgG2a/ IgG1 ratios in mice sera following YF 17 DD (panels A - C), 17D SIV Gag _45-269 (panels D - F) and 17D Δ IRES (panels G - I) virus immunization in C57BL/6 and BALB/c mice. Statistical analysis was performed by Mann-Whitney T test. * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001. The medians are representative of three independent experiments.</p