Additional file 1: of Synergic effects between ocellatin-F1 and bufotenine on the inhibition of BHK-21 cellular infection by the rabies virus

Mass spectrometry profile of the active fraction (F11) from L. labyrinthicus skin secretion. Left black arrows indicate the charge states of the 2547.99 Da molecule and right white arrows indicate the charge states of the 2192.81 Da peptide. (PDF 23 kb

Additional file 4: of Synergic effects between ocellatin-F1 and bufotenine on the inhibition of BHK-21 cellular infection by the rabies virus

Evaluation of cytotoxicity of different molecules in BHK-21 cell line after treatment with: ( A ) F11 (1 mg.mL −1 ); ( B ) OF1 (1 mg.mL −1 ); ( C ) OF1TP (6 mg.mL −1 ); (D) RGVTP (6 mg.mL −1 ) and ( E ) bufotenine (0.8 mg.mL −1 ). (F) Negative control (MEM-FBS only) and (G) positive control [DMSO (1:5) only]. Magnification 100 × . (PDF 89 kb

Additional file 3: of Synergic effects between ocellatin-F1 and bufotenine on the inhibition of BHK-21 cellular infection by the rabies virus

Figure showing direct immunofluorescence (DIF) of BHK-21 cell monolayer after treatment with RABV and: ( A ) 4 mg.mL −1 synthetic ocellatin-F1 tetrapeptide (OF1TP); ( B ) 4 mg.mL −1 synthetic rabies virus glycoprotein G tetrapeptide (RVGTP); ( C ) negative control (MEM-FBS only) and ( D ) positive control (PV only). Magnification 200 × . (PDF 35 kb

Additional file 2: of Synergic effects between ocellatin-F1 and bufotenine on the inhibition of BHK-21 cellular infection by the rabies virus

CID MS2 fragmentation spectra of ( A ) m/z = 850.16 [M + 3H+] 3+ present in F11 and ( B ) the triply charged precursor of synthetic ocellatin-F1 (OF1) (m/z = 850.25). The most evident peaks are annotated according to the fragmentation pattern. (PDF 34 kb