SUPPORTIVE TREATMENT OF VASCULAR DYSFUNCTION IN PEDIATRIC SUBJECTS WITH OBESITY: THE OBELIX STUDY

INTRODUCTION: Overweight and obese children develop abnormal endothelial cell dysfunction with increased vasomotor tone and inflammation. Curcumin, resveratrol, zinc, magnesium, selenium, soy, folic acid, and Vitamin D have shown potential beneficial effects on endothelial function. We test, among overweight and obese pediatric subjects, the effects on the endothelium of a combination of curcumin, resveratrol, zinc, magnesium, soy, folic acid, selenium, and Vitamin D. METHODS: 48 subjects (6-17 years) were randomized into two groups (placebo Vs treatment) and attended three visits at 0, 3, and 6 months (+/- 15 days). Endothelial function was assessed through the Laser Doppler Flowmetry technique using “post occlusive release hyperemic test” (PORH) for estimation of Delta Flow (DF) and Hyperemic Area under Curve index (Hyperemic AUC index) and “heat provocation test” (HPT) to measure Delta Flow HPT” (DFHPT). RESULTS: DF, Hyperemic AUC index and DFHPT represent recognized parameters for estimating endothelial dysfunction in both pediatric and adult age. Significant DF difference was noted at time-6-month in both groups (p<0.001). Overall time trend was significantly different between baseline, time-3-months, and 6-months both in Placebo (p<0.05) and Treatment (p< 0.001) groups and their comparison (p < 0.001). No differences were noted in Hyperemic AUC index (3 and 6 months), while there were significant differences in time trends of Treatment (p< 0.001) and Placebo (p<0.05) groups and their comparison (p < 0.001). DFHPT difference between groups was significant at 3 months and 6 months (p<0.05). The overall time trend was significant exclusively in "Treatment group" between time-3-month and 6-months (p<0.05). Correlation with anthropometrics was found for DF and BMI (r=0.677 at 6 months; p<0.05), as well as for Hyperemic AUC index and males (r= 0.348, p<0.05), while DFHPT showed no correlation. CONCLUSIONS: Curcumin, resveratrol, zinc, magnesium, soy, folic acid, selenium, and Vitamin D appear to be promising in enhancing endothelial function by improvement of both DF in post-occlusive release hyperemia test and DF in the heat provocation test, potentially lowering the risk of developing cardiovascular diseases in overweight and obese pediatric subjects

The psychophysical impact that COVID-19 has on children must not be underestimated

Italy has been one of the European countries that has been most affected by the COVID\u201019 pandemic. By 16 April 2020, 159,107 Italian residents had tested positive for COVID\u201019 and these included 1,123 children, up to nine years of age (0.7%) and 1,804 adolescents, aged between 10 and 19 years old (1.1%) (1). These data were in line with the case studies reported for the Chinese population, where the respective percentage (proportion) was 0.9% and 1.2% respectively (2). A five\u2010year\u2010old Italian child, who had been affected by many previous and unspecified pathologies, died after testing positive for COVID\u201019 infection (1). The lower vulnerability of the paediatric population to COVID\u201019 seems eviden

Rapid prototyping for enhanced dynamic positioning systems

The paper aims to show the design procedure for a Class-2 dynamic positioning system, from initial conceptualisation to factory assessment test. The approach involves the use of simulation-based design combined with hardware in the loop testing. This kind of approach involves a detailed knowledge of the investigated ship but gives significant well-known advantages. The main are: the reduction of the design duration, the optimisation of the system performance, the debug of the control logics before the installation onboard, the reduction of the sea trial costs. Finally, the result of this process is a product ready to be installed onboard. Moreover, over the years, the interest of the classification societies in simulation results for certifying such systems is increased. This study shows both the approach and the results of designing a dynamic positioning system for a Platform Supply Vessel. A custom simulation platform has been developed to have realistic feedbacks of the case-study ship. The DP controller structure, including regulator, force and thrust allocation; have been conceptualised, and then, after the porting procedure, the DP software has been downloaded in the real PCL. Several HIL tests have been carried out to fine-tune the controller parameters. The results show, under different environmental conditions, the respect of the design criteria

The Importance of Stereochemically Active Lone Pairs For Influencing Pb II and As III Protein Binding

The toxicity of heavy metals, which is associated with the high affinity of the metals for thiolate rich proteins, constitutes a problem worldwide. However, despite this tremendous toxicity concern, the binding mode of As III and Pb II to proteins is poorly understood. To clarify the requirements for toxic metal binding to metalloregulatory sensor proteins such as As III in ArsR/ArsD and Pb II in PbrR or replacing Zn II in δ‐aminolevulinc acid dehydratase (ALAD), we have employed computational and experimental methods examining the binding of these heavy metals to designed peptide models. The computational results show that the mode of coordination of As III and Pb II is greatly influenced by the steric bulk within the second coordination environment of the metal. The proposed basis of this selectivity is the large size of the ion and, most important, the influence of the stereochemically active lone pair in hemidirected complexes of the metal ion as being crucial. The experimental data show that switching a bulky leucine layer above the metal binding site by a smaller alanine residue enhances the Pb II  binding affinity by a factor of five, thus supporting experimentally the hypothesis of lone pair steric hindrance. These complementary approaches demonstrate the potential importance of a stereochemically active lone pair as a metal recognition mode in proteins and, specifically, how the second coordination sphere environment affects the affinity and selectivity of protein targets by certain toxic ions. Experimental and computational methods have been employed to study the influence of the lone pair of As III and Pb II for the binding of these ions in proteins using designed peptide models. The results show that the mode of coordination of As III and Pb II is greatly influenced by the steric bulk within the second coordination environment of the metals (see figure).Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90414/1/chem_201102786_sm_miscellaneous_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/90414/2/2040_ftp.pd

Vitamin D Supplementation in the Assessment of Cardiovascular Risk Factors in Overweight and Obese Children

Childhood obesity is associated with cardiovascular-disease (CVD) risk factors, an unfavorable lipid profile and reduced levels of 25(OH)D. The aim of our study is to evaluate whether vitamin D supplementation may play a role in the assessment of the CVD risk factors in overweight/obese children and adolescents

Inflammatory myofibroblastic lung tumor: its birth, its bleeding growth, its difficult diagnosis and its surgical end in a child

The diagnosis of inflammatory myofibroblastic tumor is based on radiology and histology. The treatment is surgical, and the prognosis is good. For this reason, although this lung disease is rare, when a child show up at hospital with an unknown hemoptysis, this medical condition should not be underestimated

Massive thymic hyperplasia in a 15-month-old boy: Case report and literature review

Key Clinical Message A surgical approach is the choice in young infants with MTH, who are furthest from the time of physiological involution of the thymus, and when the thymus achieves the largest relative size, a surgical approach is the choice. Steroid therapy has been shown to be ineffective (4, 9, 16, 18-20). No surgical complications have been reported, and the outcome is excellent. Recurrence has been seen in only one case

Long COVID-19 in Children: From the Pathogenesis to the Biologically Plausible Roots of the Syndrome

Long Coronavirus disease-19 (COVID-19) refers to the persistence of symptoms related to the infection with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). This condition is described as persistent and can manifest in various combinations of signs and symptoms, such as fatigue, headache, dyspnea, depression, cognitive impairment, and altered perception of smells and tastes. Long COVID-19 may be due to long-term damage to different organs-such as lung, brain, kidney, and heart-caused by persisting viral-induced inflammation, immune dysregulation, autoimmunity, diffuse endothelial damage, and micro thrombosis. In this review, we discuss the potential and biologically plausible role of some vitamins, essential elements, and functional foods based on the hypothesis that an individual's dietary status may play an important adjunctive role in protective immunity against COVID-19 and possibly against its long-term consequences

Mechanistic Analysis of Nucleophilic Substrates Oxidation by Functional Models of Vanadium-Dependent Haloperoxidases: A Density Functional Theory Study

Density functional theory has been used to investigate the structural, electronic, and reactivity properties of an established functional model for vanadium-dependent haloperoxidases, K[VO(O 2 )Hheida] (Hheida 2– = 2,2′-[(2-hydroxyethyl)imino]diacetate). Possible solution species were determined on the basis of potential exogenous donors present under the conditions necessary for reactivity. The energetically favored solution-state species is a 1:1 complex of Hheida and vanadium with a coordinated hydroxyethyl donor trans to the vanadium–oxido bond which is in agreement with the reported solid-state structure for K[VO(O 2 )Hheida]. Transition states of the oxidation reaction were located for four substrates: chloride, bromide, iodide, and dimethyl sulfide. The role of protonation and its effects on reactivity were examined for each substrate. Protonation of the peroxido moiety leads to a significant drop in the activation barrier for oxidation. In contrast no transition states could be located for an oxido-transfer process involving the oxido ligand. Barriers of activation calculated for halide oxidation were similar, providing support to the hypothesis that the p K a of the halide in acetonitrile is responsible for the decrease in reactivity between I – , Br – , and Cl – . The results presented herein provide a mechanistic correlation between a functional model and the enzyme, making K[VO(O 2 )Hheida] a “complete” functional model for vanadium-dependent haloperoxidase.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/55972/1/515_ftp.pd