4 research outputs found
Anti-leishmanial activity of alkaloidal extract from Aspidosperma ramiflorum
Infections due to protozoa of the genus Leishmania are a major
worldwide health problem, with high endemicity in developing countries.
The drugs of choice for the treatment of leishmaniasis are the
pentavalent antimonials (SbV), which present renal and cardiac
toxicity. Besides, the precise chemical structure and mechanism of
action of these drugs are unknown up to date. In order to find new
drugs against leishmaniasis, we have been studying extracts of
Brazilian trees. In the present study, we have evaluated the
effectiveness of an alkaloid extract of Aspidosperma ramiflorum
Muell. Arg. (Apocynaceae), against the extracellular forms
promastigotes of L. (L.) amazonensis and L. (V.) braziliensis. The
alkaloid extract of A. ramiflorum was much more effective against L.
(L.) amazonensis (LD50 < 47 μg/ml) than L. (V.) braziliensis.
Based on these in vitro results against L. (L.) amazonensis new studies
should be made to find the compounds with anti-leishmanial activity
Sugar content in nectar flowers of siratro (Macroptilium atropurpureum Urb.) - DOI: 10.4025/actascianimsci.v27i1.1248
Siratro (Macroptilium atropurpureum Urb.) é uma planta forrageira com alto valor nutricional e excelente palatabilidade, sua fenologia é pouco conhecida. Objetivando melhorar o conhecimento e compreensão dos polinizadores do siratro e sua biologia floral, o conteúdo de açúcares totais no néctar de suas flores e a identificação desses açúcares foram determinados por espectrofotometria e cromatografia, respectivamente. A concentração de açúcares totais variou de 1,36 a 3,23 mg/flor para o valor máximo e de 0,19 a 0,42 mg/flor para o valor mínimo. Os resultados mostraram que a concentração de açúcares totais é alta às 8h30min, quando as flores estão abertas e varia um pouco durante o tempo que as flores permanecem abertas. A variação pode estar relacionada ao número de insetos que visitam as flores, especialmente as abelhas que podem coletar pólen e néctar durante o período de antese (8h30min às 16h30min). Por meio de análise enzimática, foi verificado que o siratro possui somente a glicose na sua composiçãoSiratro (Macroptilium atropurpureum Urb.) is a forager with high nutritional values and excellent palatability, and its phenology is almost unknown. Aiming at improving the knowledge and understanding siratro pollinators and floral biology, the total sugar contents on its flowers nectar and the identification of these sugars were determined by, respectively, spectrophotometry and chromatography. The total sugar concentration varied from a maximum of 1.36 and 3.23 mg/flower and a minimum of 0.19 and 0.42 mg/flower. Results showed that the total sugar concentration is high at 8:30 a.m., when the flowers open, and varies slightly during the time the flowers keep open. The variations can be related to the number of insects that visit the flowers, especially bees that may collect pollen and nectar during the open period (8:30 a.m. to 4:30 p.m.). Through enzymatic analysis, data showed that siratro has only glucose in its compositio