X-ray Study Of Herbaspirilum Seropedicae Glnb Protein Adsorbed On Silicon

GlnB-Hs protein is a globular member of PII-like family involved in the nitrogen control fixation in diazothoph organisms like H. seropedicae. In this work, GlnB-Hs is deposited by spin-coating on Si (in) and its self-assembling is investigated by AFM (atomic force microscopy) and X-ray at grazing incidence angles. GlnB-Hs forms circular 100 nm to 150 nm wide face-up donut shaped protein aggregates on silicon. Reflectivity and diffraction profiles from the protein deposits on Si(111) and Si(100), suggest ordered domains that are stable under the X-ray beam and have d-spacing compatible with protein dimensions already determined by classical protein crystallography. Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA.245-246140146Gray, J., Curr. Opi. in Struct. Biology, 1004 (14), p. 110Sleytr, U., Gyorvary, E., (2003) Progr. in Org. Coating, 47, p. 279Pechkova, E., Nicolini, C., (2004) Trends in Bio, 22, p. 117Pechkova, E., Nicolini, C., (2001) J. of Crystal growth, 231, p. 599Giegé, R., Drenth, J., Ducruix, A., McPherson, A., Saenger, W., (1995) Prog. Cryst. Growth and charact, 30, p. 237Jap, B.K., Zulauf, M., Scheybani, T., Hefti, A., Baumeister, W., Aebi, U., Engel, A., (1992) Ultramicroscopy, 46, p. 45Als-Nielsen, J., Jacquemain, D., Kjaer, K., Leveiller, F., Lahav, M., Leiserowitz, L., (1994) Physics reports, 246, p. 251Samant, M.G., Brown, C.A., Gordon II, J.C., (1991) Langmuir, 7, p. 437Seul, M., Eisenberger, P., McCOnnell, H.M., (1983) PNAS, 80, p. 5795Fenter, P., Eisenberger, P., Li, J., Camillone, N., Bernasek, S., Scoles, G., Ramanarayanan, T.A., Liang, K.S., (1991) Langmuir, 7, p. 2013Salditt, T., Mennicke, U., (2002) Longmuir, 18, p. 8172Verclas, S.A.W., Howes, P.B., Kjaer, K., Wurlitzer, A., Weygand, M., Buldt, G., Dencher, N.A., Lösche, M., (1999) J.M.B, 287, p. 837Lawrence, C.J., Zhou, W., (1991) J. of Non-Newtonian fluid Mech, 39, p. 137Ninfa, A.J., Atkinson, M.R., (2000) Trends Microbiol, 8, p. 172Baldani, V.L.D., Baldani, J.I., Olivares, F., Dobereiner, J., (1992) Symbiosis, 13, p. 65Baldani, J.I., Baldani, V.L.D., Seldin, L., Dobereiner, J., (1986) Int. J. Syst. Bacteriol, 36, p. 86Pimentel, J.P., Olivares, F., Pitard, R.M., Urquiaga, S., Akiba, F., Dobereiner, J., (1991) Plant Soil, 137, p. 61Weber, O.B., Baldani, V.L.D., Teixeira, K.R.S., Kirchhof, C., Baldani, J.I., Doberreiner, J., (1999) Plont Soil, 210, p. 103Benelli, E.M., de Souza, E.M., Funayama, S., Rigo, L.U., Pedrosa, F.O., (1997) J. Bacteriol, 179, p. 4623de Souza, E.M., Pedrosa, F.O., Drummond, M.H., Rigo, L.U., Yates, M.G., (1999) J. Bacteriol, 181, p. 681Benelli, E.M., Buck, M., de Souza, E.M., Yates, M.G., Pedrosa, F.O., (2001) Can. J. Microbiol, 47, p. 309Benelli, E.M., Buck, M., Polikarpov, I., de Souza, E.M., Cruz, L.M., Pedrosa, F.O., (2002) Eur. J. Biochem, 269, p. 3296Hirose, M., Yasaka, T., Takakura, M., Miyazaki, S., (1991) Solid State Technology, p. 43Windt, D.L., (1998) Computers in physics, 12, p. 360Lubambo, A.F., Benelli, E.M., Klein, J., Schreiner, W., de Camargo, P.C., (2006) Cell Bio. and Biophysics, 44, p. 503Guinier, A., (1963) X-Ray Diffraction, p. 121. , Freeman, San FranciscoPechkova, E., Fiodoro, S., Fontani, D., Nicolini, C., (2005) Acta Crysta. D, 61, p. 809Adachi, E., Nagayama, K., (1997) Adv. Biophys, 34, p. 81Kralchevsky, P., Denkov, N.D., (2001) Cur. Opi. In Colloid & Inter. Sci, 6, p. 383Ravelli, R., McSweeneney, S.M., (2000) Structure, 8, p. 315Nave, C., Hill, M.A., (2005) J. Sync. Rad, 12, p. 261Ashley, J.C., (1980) IEEE Trans. on Nucl. Scien, 27, p. 1454M. J. Berger, J. S. Coursey, M. A. Zucker. V 1.2.2., NIST, Gaithersburg, MD, USA 2000Hovington, P., Drouin, D., Gauvin, R., (1997) Scanning, 19, p. 1Murray, J.W., Garman, E.F., Ravelli, R.B.G., (2004) J. of Appl. Cryst, 37, p. 51