117 research outputs found
Efficiency detection of adiponectin expression in chicken preadipocytes.
<p>(A) GFP observed by fluorescence microscopy 24 h after transfection with pGPU6 recombinant vectors. Scale bar, 100 µm. (B) The expression of <i>adiponectin</i> gene 24 h after transfection with pcDNA3.1-ADPN and pGPU6 recombinant vectors (n = 3). (C) The expression of adiponectin protein at day 2, 3 and 9 after transfection with pcDNA3.1-ADPN and siRNA-3 (n = 3). CK: Control group, pC: pcDNA3.1, pA: pcDNA3.1-ADPN, pG: pGPU6/GFP/Neo, siRNA-1: pGPU6/GFP/Neo-ADPN-676, siRNA-2: pGPU6/GFP/Neo-ADPN-751, siRNA-3: pGPU6/GFP/Neo-ADPN-952, siGH: pGPU6/GFP/Neo- siGAPDH. Values are means ± SEM. vs. control group, * <i>P</i><0.05, ** <i>P</i><0.01.</p
Adiponectin activates the p38 MAPK/ATF-2 pathway in cultured chicken preadipocytes.
<p>(A) Cells were treated either with recombination vectors alone or with 10 µM SB253580(SB), total proteins were extracted at 30 min after administration of SB253580 and then immunoblotted for total p38MAPK, phospho-p38MAPK (pT180/pY182), total ATF-2 and phospho-ATF-2 (pT71) (n = 3). (B) Representative images of Oil Red O-stained sections of cells at d 9 after treated either with recombination vectors alone or with 10 µM SB253580. (C) Lipid accumulation was assessed by the quantification of A<sub>510</sub> in destained Oil Red O with isopropyl alcohol (n = 3). Scale bar, 100 µm. CK: Control group, pC: pcDNA3.1, pA: pcDNA3.1-ADPN, pG: pGPU6/GFP/Neo, siRNA-3: pGPU6/GFP/Neo-ADPN-952, siGH: pGPU6/GFP/Neo-GAPDH. Values are means ± SEM. vs. control group, * <i>P</i><0.05, ** <i>P</i><0.01. vs. SB253580 treatment group, # <i>P</i><0.05, ## <i>P</i><0.01.</p
Effects of adiponectin on sequential expression of lipogenesis genes and proteins.
<p>(A) Expression levels of adipogenesis genes at day 1, 3 and 9 after transfection with pcDNA3.1-ADPN and siRNA-3 (n = 3). (B) Expression levels of adipogenesis proteins at day 1, 3 and 9 after transfection with pcDNA3.1-ADPN and siRNA-3 (n = 3). CK: Control group, pA: pcDNA3.1-ADPN, siRNA-3: pGPU6/GFP/Neo-ADPN-952. Values are means ± SEM. vs. control group, * <i>P</i><0.05, ** <i>P</i><0.01.</p
List of genes examined and their corresponding accession numbers.
<p>Footnote: F and R indicate forward and reverse primers respectively. <i>GAPDH  =  Glyceraldehyde 3-phosphate de-hydrogenase</i>, <i>C/EBPα  =  CCAAT/enhancer binding protein alpha</i>, <i>PPARγ  =  Peroxisome proliferator-activated receptor gamma</i>, <i>FAS  =  fatty acid synthase, ATGL  =  adipose triglyceride lipase</i>.</p
Effects of adiponectin on morphology changes and lipid metabolism of cultured chicken preadipocytes.
<p>(A) Representative images of Oil Red O-stained sections of three groups at day 1, 3 and 9. Scale bar, 100 µm. (B) Lipid accumulation was assessed by the quantification of A<sub>510</sub> in destained Oil Red O with isopropyl alcohol (n = 3). (C) The areas stained with Oil Red O assessed (n = 3). (D) Lipid droplet diameter frequency distributions for the three groups. Numbers on the x-axis represent the bins for droplets of specific sizes. (E) Glycerol content in the medium (n = 3). (F) FFA content in the cell culture medium (n = 3). FFA: free fatty acid, CK: Control group, pA: pcDNA3.1-ADPN, siRNA-3: pGPU6/GFP/Neo-ADPN-952. Values are means ± SEM. vs. control group, * <i>P</i><0.05, ** <i>P</i><0.01.</p
Free-Standing Atomically Thin ZnO Layers via Oxidation of Zinc Chalcogenide Nanosheets
Monolayer
ZnO represents a class of new two-dimensional (2D) materials
that are expected to exhibit unique optoelectronic properties and
applications. Here we report a novel strategy to synthesize free-standing
atomically thin ZnO layers via the oxidation of hydrothermally grown
ultrathin zinc chalcogenide nanosheets. With micrometer-scaled lateral
size, the obtained ultrathin ZnO layer has a thickness of ∼2
nm, and the layered structure still maintained well after high temperature
oxidation. The thermal treatment strongly improves the crystal quality
as well without inducing cracks or pinholes in the ultrathin layers.
The atomically thin ZnO layers are highly luminescent with dominant
green emission. High quality white light is obtained from the mixed
phosphors containing the ZnO layers, exhibiting their potential as
compelling ultraviolet-excited phosphors
Phosphine-Catalyzed Enantioselective [4 + 2] Cycloaddition–Semipinacol-Type-Rearrangement Reaction of Morita–Baylis–Hillman Carbonates
The chiral phosphine-triggered electrophilic
ylide intermediate
for a Morita–Baylis–Hillman carbonates activation strategy
provides a promising method for the design of organocatalytic intermolecular
higher-order annulation processes
Effects of Organic Cation Length on Exciton Recombination in Two-Dimensional Layered Lead Iodide Hybrid Perovskite Crystals
In recent years, 2D layered organic–inorganic
lead halide
perovskites have attracted considerable attention due to the distinctive
quantum confinement effects as well as prominent excitonic luminescence.
Herein, we show that the recombination dynamics and photoluminescence
(PL) of the 2D layered perovskites can be tuned by the organic cation
length. 2D lead iodide perovskite crystals with increased length of
the organic chains reveal blue-shifted PL as well as enhanced relative
internal quantum efficiency. Furthermore, we provide experimental
evidence that the formation of face-sharing [PbI<sub>6</sub>]<sup>4–</sup> octahedron in perovskites with long alkyls induces
additional confinement for the excitons, leading to 1D-like recombination.
As a result, the PL spectra show enhanced inhomogeneous broadening
at low temperature. Our work provides physical understanding of the
role of organic cation in the optical properties of 2D layered perovskites,
and would benefit the improvement of luminescence efficiency of such
materials
Discovery of <i>N</i>‑(Naphthalen-1-yl)‑<i>N</i>′‑alkyl Oxalamide Ligands Enables Cu-Catalyzed Aryl Amination with High Turnovers
A class of <i>N</i>-(naphthalen-1-yl)-<i>N</i>′-alkyl oxalamides have been proven to be powerful
ligands,
making a coupling reaction of (hetero)Âaryl iodides with primary amines
proceed at 50 °C with only 0.01 mol % of Cu<sub>2</sub>O and
ligand as well as a coupling reaction of (hetero)Âaryl bromides with
primary amines and ammonia at 80 °C with only 0.1 mol % of Cu<sub>2</sub>O and ligand. A wide range of coupling partners work well
under these conditions, thereby providing an easy to operate method
for preparing (hetero)Âaryl amines
Simple Approach to Improving the Amplified Spontaneous Emission Properties of Perovskite Films
Organo-lead halide
perovskite has emerged as a promising optical gain media. However,
continuous efforts are needed to improve the amplified spontaneous
emission (ASE) even lasing properties to evade the poor photostability
and thermal instability of the perovskites. Herein, we report that
simply through the coating of polymer layer, the CH<sub>3</sub>NH<sub>3</sub>PbBr<sub>3</sub> polycrystalline films prepared by a modified
sequential deposition process show remarkably enhanced photoluminescence
and prolonged decay lifetime. As a result, under nanosecond pulse
pumping, the ASE threshold of the perovskite films is significantly
reduced from 303 to 140 μJ/cm<sup>2</sup>. Furthermore, the
light exposure stability is improved greatly after the polymer coating.
We confirmed that the polymer layer plays the roles of both surface
passivation and symmetric waveguides. Our results may shed light upon
the stable and sustained output of laser from perovskite materials
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