Fabrication of mAb G250-SPIO molecular magnetic resonance imaging nanoprobe for the specific detection of renal cell carcinoma in vitro.

Molecular magnetic resonance imaging (mMRI) has been paid more and more attention for early diagnosis of cancer. A sensitive and specific mMRI probe plays the most important role in this technique. In this study, superparamagnetic iron oxide (SPIO) nanoparticles and mAb G250 were conjugated as mMRI probe for the detection of clear cell renal cell carcinoma (ccRCC) using 3.0-Tesla MRI in vitro. mAb G250 could specifically recognize carbonic anhydrase IX (CAIX) antigen overexpressed in ccRCC and the SPIO nanoparticles as MRI contrast agent presented excellent MRI response and good biocompatibility. The successful assembly of this nanoprobe was confirmed by UV-vis spectrum, FT-IR spectroscopy and DLS analysis. In vitro MRI study on ccRCC cells and control cells indicated that our fabricated mAb G250-SPIO nanoprobe could be used in the specific labeling of clear cell renal carcinoma cells successfully

Measuring Subthalamic Nucleus Volume of Parkinson’s Patients and Evaluating Its Relationship with Clinical Scales at Pre- and Postdeep Brain Stimulation Treatment: A Magnetic Resonance Imaging Study

This study investigated potential imaging biomarkers for predicting the efficacy of deep brain stimulation (DBS) of the subthalamic nucleus (STN) in patients with Parkinson’s disease (PD). A total of 59 PD patients and 50 healthy control subjects underwent high-resolution 3-dimensional T1-weighted brain magnetic resonance imaging. Bilateral STN volumes were compared between the 2 groups, and a correlation analysis was performed to assess the relationship between bilateral STN volumes or intracranial volume (ICV) and pre- or postoperative clinical scale scores. The results showed that the left STN volume differed significantly between PD patients and controls. In patients, the left STN volume was negatively correlated with pre- and postoperative quality of life scores and positively correlated with Mini-mental State Examination (MMSE) and Montreal Cognitive Assessment scores; ICV was also positively correlated with the MMSE score. These findings indicate that changes in the left STN volume are a useful biomarker for evaluating the clinical outcome of PD patients following DBS

FT-IR spectra of mAb G250-SPIO nanoprobes (green line), SPIO nanoparticles (black line), and mAb G250 (red line).

<p>FT-IR spectra of mAb G250-SPIO nanoprobes (green line), SPIO nanoparticles (black line), and mAb G250 (red line).</p

Characterization of SPIO nanoparticles.

<p>(A) TEM image of SPIO nanoparticles. (B) Distribution of SPIO nanoparticle size. (C) T2-weighted MR image of SPIO nanoparticles with different concentrations at 3.0 T. With increasing SPIO concentration, the MR image became darker.</p

T2-weighted and T2map MR images of 786

<p>-<b>0 renal carcinoma cells treated with mAb G250-SPIO nanoprobe, IgG-SPIO nanoprobe, SPIO nanoparticles, or culture medium.</b></p

Cell viability of 786-0 renal carcinoma cells (A) and normal human umbilical vein endothelial cells (B) after exposure to various concentrations of SPIO nanoparticles, as determined by a CCK-8 assay.

<p>Cell viability of 786-0 renal carcinoma cells (A) and normal human umbilical vein endothelial cells (B) after exposure to various concentrations of SPIO nanoparticles, as determined by a CCK-8 assay.</p

UV-vis absorption spectra of mAb G250-SPIO nanoprobes (blue line), SPIO nanoparticles (red line), and mAb G250 (black line).

<p>The emergence of absorption peak of mAb G250 in the mAb G250-SPIO nanoprobe indicates the successful fabrication of specific MR imaging nanoprobe.</p

T2-weighted (A) and T2map MR images (B) of NIH-3T3, HLF-1 and HUVEC cells treated with G250-mAb-SPIO nanoprobe, BSA-blocked SPIO nanoparticles, or cell culture medium.

<p>T2-weighted (A) and T2map MR images (B) of NIH-3T3, HLF-1 and HUVEC cells treated with G250-mAb-SPIO nanoprobe, BSA-blocked SPIO nanoparticles, or cell culture medium.</p

MRI stability test of BSA-blocked SPIO nanoparticles in cell culture medium for various hours. (1) BSA-blocked SPIO nanoparticles were dispersed in PBS for 1 h.

<p>(2)–(4) BSA-blocked SPIO nanoparticles were dispersed in cell culture medium for 1 h, 5 h, and 24 h. (5) pure 30% gelatin solution.</p

T2 relaxation time of NIH-3T3 cells, HLF-1 cells and HUVEC cells treated with G250-mAb-SPIO nanoprobe, BSA-blocked SPIO nanoparticles or culture medium.

<p>T2 relaxation time of NIH-3T3 cells, HLF-1 cells and HUVEC cells treated with G250-mAb-SPIO nanoprobe, BSA-blocked SPIO nanoparticles or culture medium.</p