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Additional file 3. Effect of inhibitors on the cellulosomalcomplexes. The glucose concentration was measured using the Glu-HK determination kit (Megazyme). The residual complex activity was assessed using 0.5 mL standard reaction mixture containing 0.25% (w/v) of the substrate for one to 2 days of incubation at 60 °C. The initial activity at time point 0 corresponds to 100% relative activity

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Additional file 4. Overview of 47 recombinant proteins. All proteins were mixed in equimolar amounts before adding them to the pentavalent SKLMY complex

MOESM1 of Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization

Additional file 1. Oligonucleotides used in this study

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Additional file 2. SDS-PAGE summary of all recombinantly expressed dockerin type I containing proteins used in this study (60 different components of C. thermocellum cellulosome). Each protein is shown after the purification process, including the final heat precipitation step. Enzyme names and Clo1313 numbers are shown above the corresponding SDS-PAGE picture. Molecular weight standards are depicted in kDa. Proteins marked with asterisks are truncated versions of the original protein