Microneutralization (MN) and hemagglutination inhibition (HAI) titres are highly correlated for seasonal influenza subtypes H1N1 and H3N2.

<p>Log-transformed MN and HAI titres for subtypes A) H1N1 (A/Brisbane/59/2007) and B) H3N2 (A/Brisbane/10/2007) are presented, along with the significance of correlation as determined by linear regression.</p

BT3.2 expression in ovarian cancer cells.

<p><b>A</b>. Western-blot analysis of total protein extracts from TOV112D cell line infected with PLenti (vector control), BT3.1, BT3.2 or BT3.3 viral constructs. Extracts were loaded in triplicate on 10% SDS/PAGE gel and membranes were hybridized with anti-CD277 (eBioscience), anti-BT3.3 (Atlas Antibodies) and anti-BT3.2 (SDIX Inc.) as indicated at the bottom of the Figure. <b>B</b>. Immunohistochemistry analysis of paraffin-embedded cell pellets from TOV112D cell line infected with either PLenti (vector control), BT3.1, BT3.2 or the BT3.3 viral constructs. Only cells transfected with the BT3.2 construct stained positively with the anti-BT3.2 (SDIX Inc.). <b>C</b>. Immunohistochemistry of xenograft tumors from TOV112D transfected with either empty vector or BT3.2 construct. Only cells infected with the BT3.2 construct stained positively with the anti-BT3.2 (SDIX Inc.). <b>D</b>. Representative staining for immunohistochemistry of BT3.2 on a high-grade serous EOC TMA. From left to right: negative, low, moderate and high intensity.</p

Representative staining for immunohistochemistry of infiltrating macrophages.

<p>High and low density of CD68+ (tumor-associated macrophages, TAM) (A) and CD206+ (M2 subtype of TAM) cells (B). Magnification in the high infiltration area is shown on the right panel for each staining (top). C and D. Kaplan-Meier analysis of the ratio of infiltrating intraepithelial CD206+/CD68+ cells representing the relative density of CD206+ M2 TAM over the total density of CD68+ intraepithelial infiltrating macrophages. Kaplan-Meier curves of overall survival (C) and disease-free survival (D) in 180 and 174 patients, respectively. Significance (p) is indicated by log rank.</p

Pearson correlation test (two-tailed) between intra-epithelial immune infiltrate and clinical parameters in EOC patients.

<p>Table showing the coefficient of correlation with a Pearson correlation test (Pearson Correlation) between clinical parameters and immune infiltrate density in the intra-epithelial ovarian tumor tissues. Grade and stage were evaluated according to the FIGO classification. Res. Dis. =  amount of residual disease at primary resection of the tumor as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038541#pone-0038541-t001" target="_blank">Table 1</a>. Recurrence is the first event of ovarian cancer recurrence after the primary resection of the tumor. Death is the event of death due to ovarian cancer. CA125 =  blood serum level CA125 at the date of primary resection of the ovarian tumor. P is the p value from Pearson correlation test. N is the number of cases included in the statistical analysis. C =  Pearson correlation coefficient. P = p value. N =  number of cases.</p

Pearson correlation test (two-tailed) between BT3.2 expression and markers of intra-epithelial immune infiltrate in EOC tissues.

<p>Table showing the Pearson coefficient of correlation C between staining observed for each marker in the intra-epithelial ovarian tumor tissues. Grade and stage were evaluated according to the FIGO classification. P is the p value from Pearson correlation test. N is the number of cases included in the statistical analysis for which the observed staining was reliable. CD206/CD68 =  ratio of CD206+/CD68+ staining in the intra-epithelial area of tumor. C =  Pearson correlation coefficient. P = p value.</p

Representative staining for immunohistochemistry of tumor infiltrating lymphocytes.

<p>High and low density of CD3+ (T cells), CD4+ (T cells), CD8+ (T cells) and CD20+ (B cells) shown in left and middle panels. A magnification in the high infiltration area is shown on the right panel for each staining.</p

Pearson correlation test (two-tailed) between BT3.2 expression in EOC tissues and clinical parameters of patients.

<p>Table showing the coefficient of correlation with a Pearson correlation test (Correlation) between clinical parameters and BT3.2 staining intensity observed in the intra-epithelial area of ovarian tumor tissues. Grade and stage were evaluated according to the FIGO classification as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038541#s2" target="_blank">Materials and Methods</a>. Res. Disease is the amount of residual disease at primary resection of the tumor as classified in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038541#pone-0038541-t001" target="_blank">Table 1</a>. Recurrence is the first event of ovarian cancer recurrence after the primary resection of the tumor. CA125 =  blood serum level CA125 at the date of primary resection of the ovarian tumor. P is the p value from Pearson correlation test. N is the number of cases included in the statistical analysis.</p

Univariate and multivariate Cox regression analysis showing the statistical association between BT3.2 expression and outcome of EOC patients.

<p>OS =  overall survival is the time of survival from the date of primary resection until event of death due to ovarian cancer. Res. Dis. =  amount of residual disease at time of primary resection of the ovarian tumor. Age =  age of patient at time of the first resection of the ovarian tumor. DFS =  disease free survival is the time from the first resection of the primary tumor until the first event of recurrence. HR =  hazard ratio. CI =  confidence interval. P =  p value.</p

Kaplan Meier analysis of BT3.2 in high-grade serous EOC.

<p>Kaplan-Meier curves of overall survival (A) and disease-free survival (B) in 194 and 171 patients, respectively. Significance (p) is indicated by log rank.</p