Nyt 100% Proteinfoder til Økologiske Æg- og Kyllingeproduktion

Brochure om RDD1 projektet BioconVa

Præsentation af status for Bioconval til Organic RDD 14.12.12

Kort status for Bioconval projektet på statusmøde for Organic RDD projekter den 14. december 2012

Report on completed/demonstrated rearing system - Farmergødning/Bånlev

Rapport omhandlende kompostering af gødning med fluelarver på prototype

Larvae for layers

Companies and researchers are in close collaboration developing a container- based system for cultivating fly larvae at organic poultry farms. In a one week process, manure will be converted to compost and the live larvae will be harvested and used for feeding laying hens. The larvae are expected to have a beneficial effect on the growth performance, intestinal health and on animal behavior in flocks

Impact of salinomycin on the intestinal microflora of broiler chickens

<p>Abstract</p> <p>Background</p> <p>The ionophoric coccidiostat salinomycin is widely used in chicken feed. In the near future the use of ionophore coccidiostats may be banned as has been the case for other antimicrobial growth promoters. This study was conducted to examine the effect of salinomycin on <it>Campylobacter jejuni </it>infection and on the composition of the caecal microflora in broiler chickens.</p> <p>Methods</p> <p>An experimental infection study was carried out in isolators and the intestinal microflora was analyzed using quantitative cultivation, denaturant gradient gel electrophoresis (DGGE), cloning and sequencing.</p> <p>Results</p> <p>We found no effect of salinomycin on <it>C. jejuni </it>but salinomycin significantly affected the composition of the microflora. In addition, salinomycin significantly reduced the prevalence of <it>Clostridium perfringens </it>and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.</p> <p>Conclusion</p> <p>Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.</p

The influence of the cage system and colonisation of Salmonella Enteritidis on the microbial gut flora of laying hens studied by T-RFLP and 454 pyrosequencing

<p>Abstract</p> <p>Background</p> <p>In the EU conventional cages for laying hens are forbidden beginning in January 2012, however concerns about a higher transmission rate of <it>Salmonella </it>in alternative cages systems have been raised. The extent to which cage systems may affect the intestinal microbiota of laying hens is not known, and different microbiota may demonstrate different resistance towards colonization with <it>Salmonella</it>. To investigate this, ileal and caecal samples from two experimental studies where laying hens were inoculated with <it>Salmonella </it>Enteritidis and housed in different systems (conventional cage, furnished cage or aviary), were compared using Terminal Restriction Fragment Length Polymorphism (T-RFLP). The distribution of genera in the microbiota in caecum was furthermore described by next generation sequencing of 16S rDNA libraries.</p> <p>Results</p> <p>Hens in the same cage type developed similar T-RFLP fingerprints of the ileal and caecal microbiota, and these could be separated from layers in the other cages types. No significant difference in the fingerprint profiles could be observed between <it>Salmonella </it>positive and negative samples from same cage. By deep sequencing of 16S rDNA libraries from caecum, 197 different Operational Taxonomic Units (OTU) were identified, and 195 and 196 OTU respectively, were found in hens in aviary and furnished cages, but only 178 OTU of these were recovered from conventional cages. The ratio between the dominating phyla or families and genera in the microbiota remained fairly constant throughout the study. <it>Faecalibacterium </it>and <it>Butyricimonas </it>were the most prevalent genera found in the caecal microbiota of layers irrespective of the cage type.</p> <p>Conclusions</p> <p>Hens confined in the same cage group tend to develop similar microbiota in their ileum and caecum possibly due to isolation, while differences in the microbiota between cages may be caused by environmental or individual bird factors. Although the cages type had influence on composition of the microbiota in the layers by promoting higher diversity in furnished and aviary systems, we did not observe differences in colonization and excretion pattern of <it>Salmonella </it>from these groups. We suggest, that differences in group size and exposure to a more faecally contaminated environment in the alternative systems may explain the observed differences in diversity of the caecal microbiota.</p

Integreret larveproduktion til foder i økologisk ægproduktion

Præsentation af projektet og dets udfordringer ved Åbent hus dag hos Jan Volmar, 31. oktober 201

Efficiency and scalability in producing feed from manure using the common housefly

Key-note (Insects to feed the world): Efficiency and scalability in producing feed from manure using the common housefl

Co-infection dynamics of a major food-borne zoonotic pathogen in chicken

A major bottleneck in understanding zoonotic pathogens has been the analysis of pathogen co-infection dynamics. We have addressed this challenge using a novel direct sequencing approach for pathogen quantification in mixed infections. The major zoonotic food-borne pathogen Campylobacter jejuni, with an important reservoir in the gastrointestinal (GI) tract of chickens, was used as a model. We investigated the co-colonisation dynamics of seven C. jejuni strains in a chicken GI infection trial. The seven strains were isolated from an epidemiological study showing multiple strain infections at the farm level. We analysed time-series data, following the Campylobacter colonisation, as well as the dominant background flora of chickens. Data were collected from the infection at day 16 until the last sampling point at day 36. Chickens with two different background floras were studied, mature ( treated with Broilact, which is a product consisting of bacteria from the intestinal flora of healthy hens) and spontaneous. The two treatments resulted in completely different background floras, yet similar Campylobacter colonisation patterns were detected in both groups. This suggests that it is the chicken host and not the background flora that is important in determining the Campylobacter colonisation pattern. Our results showed that mainly two of the seven C. jejuni strains dominated the Campylobacter flora in the chickens, with a shift of the dominating strain during the infection period. We propose a model in which multiple C. jejuni strains can colonise a single host, with the dominant strains being replaced as a consequence of strain-specific immune responses. This model represents a new understanding of C. jejuni epidemiology, with future implications for the development of novel intervention strategies