Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-0

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>0 μl PBS (left panel) or in the same volume of serum (right panel), followed by flow cytometry. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Fluorescence 2 (FL2-H) dot-plot. The number of counts in the area containing specific signals (R2) is given in the figures. Aggregate formation in serum is strongly inhibited

Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-2

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>yzed by flow cytometry. The measurements are shown in a Fluorescence 1 (FL1-H) vs. Side-Scatter (SSC) dot-plot. All six BSE-samples (A-F) can be differentiated from the controls (G-J) by a population of events in region R3 (green dots). Panel K: Quantification of measurements shown in panels A-J

Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-1

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>(panel A) or presence (panel B) of 10nM PrP aggregates. Panel C: quantification of measurements shown in A and B, and of measurements (not shown) with different seed concentrations. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Side-Scatter (SSC) dot-plot. Aggregate formation (signal in region R1) was strongly enhanced by all seed concentrations tested, from 5 nM to 10nM