2 research outputs found
Preferential Mitochondrial Localization of a Goniothalamin Fluorescent Derivative
A fluorescent
2,1,3-benzothiadiazole-containing goniothalamin derivative,
BTDāGTN (<b>1</b>), has been synthesized and successfully
tested in bioimaging experiments in live cells. The fluorescent compound
proved to be capable of transposing the cell membranes, indicating
its subcellular localization. The use of the benzothiadiazole core
as the fluorophore revealed the favored localization of the GTN analogue <b>1</b> in the cytoplasm of live cells, preferentially in the mitochondria,
in line with previous results that indicated the loss of mitochondrial
transmembrane potential upon treatment with GTN. The results described
herein highlight the potential of the BTDāGTN hybrid structures
for future studies regarding the cellular mechanism of action of this
family of compounds
From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
The current article
describes the synthesis, characterization,
and application of a designed hybrid fluorescent BTDācoumarin
(2,1,3-benzothiadiazole-coumarin) derivative (named <b>BTD-Lip</b>). The use of <b>BTD-Lip</b> for live-cells staining showed
excellent results, and lipid droplets (LDs) could be selectively stained.
When compared with the commercially available dye (BODIPY) for LD
staining, it was noted that the designed hybrid fluorescence was capable
of staining a considerable larger number of LDs in both live and fixed
cells (ca. 40% more). The new dye was also tested on live Caenorhabditis elegans (complex model) and showed
an impressive selectivity inside the worm, whereas the commercial
dye showed no selectivity in the complex model