Maximum likelihood (ML) and Neighbor joining (NJ) phylogenetic trees of 28 core genome virulence genes.

<p>The ML and NL phylogenetic trees of both chromosomes of <i>V</i>. <i>mimicus</i> CAIM 1882, 1883, 602 and <i>V</i>. <i>mimicus</i> 451 were obtained using MEGA, where <i>V</i>. <i>cholerae</i> O1 biovar El Tor N16961 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144885#pone.0144885.ref034" target="_blank">34</a>] was used as outgroup.</p

BLAST matrix (proteome comparison) of <i>V</i>. <i>mimicus</i> 451, <i>V</i>. <i>mimicus</i> CAIM 602, <i>V</i>. <i>mimicus</i> CAIM 1882 and <i>V</i>. <i>mimicus</i> CAIM 1883.

<p>The BLAST matrix was obtained using CMG-biotools [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144885#pone.0144885.ref035" target="_blank">35</a>].</p

Genome Atlas obtained of <i>V</i>. <i>mimicus</i> CAIM 602 (green), CAIM 1882 (red), CAIM 1883 (blue) and <i>V</i>. <i>mimicus</i> 451 (as control strain).

<p>The atlas was constructed with GeneWiz Browser 0.94. From the inner ring to the outer ring: Percent AT, GC Skew, Global Inverted Repeats, Global Direct Repeats, Position Preference, Stacking Energy and Intrinsic Curvature.</p

Composite dendrogram for the three strains of <i>V</i>. <i>mimicus</i> isolated from frozen shrimp process (<i>V</i>. <i>mimicus</i> 87, 92 and 93) and <i>V</i>. <i>mimicus</i> CAIM 602T (type strain).

<p>The dendrogram and a similarity matrix were obtained by a cluster analysis of ERIC-PCR, GTG-5, RAPD and ARDRA (Bionumerics, Applied Maths, Inc).</p

Dendrograms of ERIC-PCR, RAPD, GTG-5PCR and ARDRA used for the analysis of <i>V</i>. <i>mimicus</i> 87, 92, 93 and CAIM 602 (type strain).

<p>The images were analyzed with Bionumerics software (Applied Maths, Inc.) with Dice correlation coefficient for the distance matrix and UPGMA with optimization set at 1% to create the dendrogram.</p