Recommendations for Sport and Physical Activity after total Hip and Knee Arthroplasty: A Systematic Review

Abstract Due to the high success of arthroplasty, patients suffering from osteoarthritis have higher expectations regarding the relief of pain, functional recovery and capacity to perform physical and sports activity. The purpose of our article is to present a literature review based on papers published during the last decades, with emphasis on the current recommendations regarding sports after total joint replacement. We conducted a search using PubMed/Medline databases, selecting the scientific articles published between 2005-2020 which discuss the association between physical activity and total hip or knee arthroplasty. The benefits of physical activity in all kinds of joint prostheses outweigh the negative effects; therefore, the recommendation for low-impact sports (hiking, swimming, cycling or golf) at a moderate intensity is considered valid and patients with total hip or knee arthroplasty who preoperatively had a high level of physical activity can return to low-impact sports performed at moderate intensity after 3-6 months.</jats:p

The Conversion Rate of Arthroscopy to Total Joint Arthroplasty in Patients with Knee Osteoarthritis

Abstract The efficacy of knee arthroscopy in the treatment of osteoarthritis has been questioned during the last years by many studies, which demonstrated its lack of effect. The aim of our review was to summarize recent data published in the scientific literature upon the conversion rate from arthroscopy to total arthroplasty in patients with knee osteoarthritis. We conducted a search of scientific articles discussing the association between arthroscopy and total knee arthroplasty in patients with osteoarthritis, using PubMed/Medline databases. The following parameters were evaluated: age and gender distribution of the patients, indications for arthroscopy, conversion to arthroplasty, time interval between the two procedures. We concluded that the current literature underlines the poor efficacy of arthroscopy for degenerative meniscus and cartilaginous pathology. The high rate of progression to knee arthroplasty, especially among older patients with comorbidities should determine specialists to evaluate the risks and benefits associated with arthroscopic surgery in these cases.</jats:p

Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression

Abstract Background Bladder cancer (BC) is a common urothelial malignancy, characterized by a high recurrence rate. The biology of bladder cancer is complex and needs to be deciphered. The latest evidence reveals the critical role of the non-coding RNAs, particularly microRNAs (miRNAs), as vital regulatory elements in cancer. Method We performed a miRNAs microarray using paired tissues (tumor and adjacent normal bladder tissue), followed by the validation with qRT-PCR of five selected transcripts. Additional next-generation sequencing investigation established the interconnection among the altered miRNAs and mutated genes. Based on the overlapping between TCGA data and data obtained in the study, we focused on the systematic identification of altered miRNAs and genes mutated involved in bladder cancer tumorigenesis and progression. Results By overlapping the miRNAs expression data, the two patient cohorts, we identified 18 miRNAs downregulated and, 187 miRNAs upregulated. qRT-PCR validation was completed using a selected panel of two downregulated (miR-139-5p and miR-143-5p) and three up-regulated miRNAs (miR-141b, miR-200 s or miR-205). Altered miRNAs patterns are interrelated to bladder tumorigenesis, allowing them to be used for the development of novel diagnostic and prognostic biomarkers. Three EMT-related upregulated miRNAs have an essential role in the molecular mechanisms, specifically key processes underlying tumorigenesis, invasion and metastasis. Using the Ampliseq Cancer Panel kit and Ion Torrent PGM Next-Generation Sequencing an increased mutation rate for TP53, FGFR3, KDR, PIK3CA and ATM were observed, but the mutational status for only TP53 was correlated to the survival rate. The miRNAs pattern, along with the gene mutation pattern attained, can assist for better patient diagnosis. Conclusion This study thereby incorporates miRNAs as critical players in bladder cancer prognosis, where their altered gene expression profiles have a critical biological function in relationship with tumor molecular phenotype. The miRNA-mRNA regulatory networks identified in BC are ripe for exploitation as biomarkers or targeted therapeutic strategies. </jats:sec

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Additional file 8: Figure S3. A Network analysis of the mutated genes in relation to their targeted miRNAs in bladder cancer. Schematic representation was obtained from miRnet ( https://www.mirnet.ca/ )

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Additional file 2: Table S2. Demographic and histopathological characteristics of TCGA bladder cancer patient cohort