People of the Lakes

Publisher PD

"Walking Threads, Threading Walk" : Weaving and Entangling Deleuze and Ingold with Threads

Acknowledgements I want to thank all the participants of the Walking Threads Event (this also includes the wind, the trees, the branches, by-passers, the thread, etcetera). I thank The Unfamiliar editors and reviewers. I want to particularly acknowledge Valeria Lembo whose questions and discussions formed the inspiration and encouragement to engage more intensively with Ingold and Deleuze. The article was made possible through the financial support of ERC Advanced Grant Arctic Domus. Any shortcomings are mine.Peer reviewedPublisher PD

"Walking Threads, Threading Walk": Weaving and Entangling Deleuze and Ingold with Threads

In this paper, I explore theoretical discussions that have emrged through the Walking Threads exercise, correspondences with Lembo, philosophical treatises by Deleuze and Guattari, and anthropological works by Ingold. The subsequent theoretical exploration has been an attempt in weaving together all these different correspondences by walking in the theoretical imaginations of Ingold and Deleuze. Walking Threads, I conclude, can be considered as an exercise or way of incorporating theory into practice

Introducing the Walking Threads Project

The following collections of essays and creative interventions of Paola Esposito, Ragnhild Freng Dale, Jan Peter Laurens Loovers, and Brian Schultis are the result of individual attempts to recall, reflect, and make sense of an exercise called "Walking threads". It also expressed a commitment to allow experiences to grow within and between the authors in an exercise of attunement

'Hard Times are Coming' : Architectures of Cultural Revitalization

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Architectures of domestication : on emplacing human-animal relations in the North

Acknowledgements. This fieldwork and discussions which led to this article was sponsored by ERC Advanced Grant 295458 Arctic Domus and ESRC grant ES‐MO110548‐1 JPI Climate Humanor. We would like to thank Rob Losey, Bente Sundsvold, and Konstantin Klokov for their comments on the manuscript. We would also like to thank that particularly engaging and constructive comments from three anonymous reviewers.Peer reviewedPublisher PD

The β6/β7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation.

Hsp70 is a highly conserved chaperone that in addition to providing essential cellular functions and aiding in cell survival following exposure to a variety of stresses is also a key modulator of prion propagation. Hsp70 is composed of a nucleotide-binding domain (NBD) and substrate-binding domain (SBD). The key functions of Hsp70 are tightly regulated through an allosteric communication network that coordinates ATPase activity with substrate-binding activity. How Hsp70 conformational changes relate to functional change that results in heat shock and prion-related phenotypes is poorly understood. Here, we utilised the yeast [PSI +] system, coupled with SBD-targeted mutagenesis, to investigate how allosteric changes within key structural regions of the Hsp70 SBD result in functional changes in the protein that translate to phenotypic defects in prion propagation and ability to grow at elevated temperatures. We find that variants mutated within the β6 and β7 region of the SBD are defective in prion propagation and heat-shock phenotypes, due to conformational changes within the SBD. Structural analysis of the mutants identifies a potential NBD:SBD interface and key residues that may play important roles in signal transduction between domains. As a consequence of disrupting the β6/β7 region and the SBD overall, Hsp70 exhibits a variety of functional changes including dysregulation of ATPase activity, reduction in ability to refold proteins and changes to interaction affinity with specific co-chaperones and protein substrates. Our findings relate specific structural changes in Hsp70 to specific changes in functional properties that underpin important phenotypic changes in vivo. A thorough understanding of the molecular mechanisms of Hsp70 regulation and how specific modifications result in phenotypic change is essential for the development of new drugs targeting Hsp70 for therapeutic purposes