The 600MHz NMR spectra of 2.0 mM ¹⁵N,¹³C-labeled RGD-hirudin (1–66) in 20 mM sodium phosphate buffer, pH 7.4.

<p>a. ¹⁵N(F1 axis)-NH(F2 axis) region of the 2D ¹⁵N-¹H Overbodenhausen correlation spectrum, b, c & d are selected ¹³C(F1)-¹H(F3) planes at δ[¹⁵N(F2)] = 116.4 ppm of the 3D CBCA(CO)NH, CBCANH and HNCO spectra of RGD-hirudin (1–66) respectively. Sequential connectivities for four residues are indicated.</p

Expression and purification of unlabeled RGD-hirudin and labeled RGD-hirudin were analyzed by 15% SDS-PAGE.

<p>Lane M, molecular mass standards (116 to 14.4 kDa ). a. Loading was 10 µL of culture supernatant of unlabeled RGD-hirudin for 0 to 20 h after methanol induction. b. Loading was 10 µL of culture supernatant of labeled RGD-hirudin for 0 to 20 h after methanol induction. c. Loading was 10 µL of the purified unlabeled RGD-hirudin (Lane 1) and labeled RGD-hirudin (Lane 2).</p

Results of ¹⁵N, ¹³C-labeled RGD-Hirudin Purification.

<p>Results of ¹⁵N, ¹³C-labeled RGD-Hirudin Purification.</p

The anti-thrombin activity of purified unlabeled and ¹⁵N, ¹³C-labeled RGD-hirudin.

<p>The anti-thrombin activity of purified unlabeled and ¹⁵N, ¹³C-labeled RGD-hirudin.</p

¹⁵N, ¹³C and ¹H Resonance Assignments for r-RGD-Hirudin at pH 7.4 and 25°C.

<p>¹⁵N, ¹³C and ¹H Resonance Assignments for r-RGD-Hirudin at pH 7.4 and 25°C.</p

Purity and molecular weight of ¹⁵N, ¹³C-labeled RGD-hirudin were determined by HPLC-MS.

<p>a. HPLC analysis of purified ¹⁵N, ¹³C-labeled RGD-hirudin showed the purity was up to 98%. b. Mass spectrometry of the purified proteins for unlabeled RGD-hirudin showed its molecular weight was 7021 Da c. The molecular weight of ¹⁵N, ¹³C-labeled RGD-hirudin was 7387 Da The total labeling ratio is more than 98%.</p

sj-docx-1-wso-10.1177_17474930231205221 – Supplemental material for The frequency of imaging markers adjusted for time since symptom onset in intracerebral hemorrhage: A novel predictor for hematoma expansion

Supplemental material, sj-docx-1-wso-10.1177_17474930231205221 for The frequency of imaging markers adjusted for time since symptom onset in intracerebral hemorrhage: A novel predictor for hematoma expansion by Lei Song, Jun Cheng, Cun Zhang, Hang Zhou, Wenmin Guo, Yu Ye, Rujia Wang, Hui Xiong, Ji Zhang, Ren Ke, Dongfang Tang, Yufei Fu, Zhibing He, Liwei Zou, Longsheng Wang, Lianghong Kuang, Xiaoming Qiu, Tingting Guo and Yongqiang Yu in International Journal of Stroke</p