Correction: Transcriptome analysis of tomato (Solanum lycopersicum L.) shoots reveals a crosstalk between auxin and strigolactone.

[This corrects the article DOI: 10.1371/journal.pone.0201124.]

Transcriptome analysis of tomato (<i>Solanum lycopersicum</i> L.) shoots reveals a crosstalk between auxin and strigolactone

<div><p>Auxin and strigolactone (SL) are two important phytohormones involved in shoot branching and morphology. Tomato (<i>Solanum lycopersicum</i> L.), a member of the Solanaceae family, is one of the most popular food crops with high economic value in the world. To seek a better understanding of the responses to exogenous hormones, transcriptome analyses of the tomato shoots treated with exogenous auxin and SL, separately or together, were performed. A total of 2326, 260 and 1379 differential expressed genes (DEGs) were identified under the IAA, GR24 and IAA+GR24 treatments, respectively. Network analysis pointed out two enriched interaction clusters, including “ethylene biosynthesis” and “photosynthesis”. Several ethylene biosynthesis and metabolism-related genes were up-regulated under both IAA and IAA+GR24 treatments, suggesting their involvement in the regulation of ethylene biosynthesis. Besides, auxin-SLs-triggered the expression of several <i>CAB</i> genes may lead to systemic increases in the induction of photosynthesis. Several auxin-activated metabolic pathways could be reduced by the GR24 treatment, indicated that the crosstalk between auxin and SLs may be involved in the metabolic regulation of tomato. Further analysis showed that SLs affect the responses of tomato shoots to auxin by inducing the expression of a series of auxin downstream genes. On the other hand, auxin regulated the biosynthesis of SLs by affecting the genes in the “Carotenoid biosynthesis” pathway. Our data will give us an opportunity to reveal the crosstalk between auxin and SLs in the shoots of tomato.</p></div

Real-time quantitative PCR validation of several hormone-related genes.

<p>The histogram shows the relative expression level of these genes with respect to the ACTIN in tomato. The data were analyzed by three independent repeats, and standard deviations were shown with error bars. Significant differences in expression level were indicated by “*”.</p

GO enrichment analysis of the DEGs in different comparisons.

<p>(a) Classification of the enriched GO terms under the IAA treatment. (b) Classification of the enriched GO terms under the GR24 treatment. (c) Classification of the enriched GO terms under the IAA+GR24 treatment.</p

Transcriptional variations in tomato shoots under different hormone treatments.

<p>(a) Expression profiles of the DEGs under different hormone treatments were shown by a heatmap. (b) Significance analysis of the DEGs in different comparisons by Volcanoplots. (c) The number of up- and down-regulated genes in different comparisons. (d) Venn diagrams showed the proportions of the up- and down-regulated genes in three comparisons.</p

Transcript abundance changes of auxin- and SL-related genes.

<p>(a) Overview of the auxin signaling pathway in tomato. (b) The numbers of the DEGs involved in the auxin signaling pathway. (c) Overview of the SL biosynthesis pathway in tomato. (d) The numbers of the DEGs involved in the SL biosynthesis pathway.</p

Interaction networks of the DEGs analyzed by Cytoscape software ver. 3.0.1.

<p>(a) The PPI network under the IAA treatment. (b) The PPI network under the IAA+GR24 treatment. (c) The PPI network under the GR24 treatment. Red background colour indicated the proteins involved in the ethylene biosynthesis pathway. Green background colour indicated the proteins involved in the photosynthesis pathway.</p