Study on parameters optimization of unmanned aerial vehicle and ecological remediation of buckwheat stained with DSE

The complex and changeable terrain, subsidence topography and land fissures of mining area in northern Shaanxi make it difficult for mechanical restoration of ecological environment. Based on M8A20A eight-rotor UAV platform, this study investigated the effects of the optimal parameters on aerial seeding, and the ecological restoration of buckwheat by DSE infection and aerial seeding. Results showed that the flight parameters had significant effects on the available width and uniformity of buckwheat seeds. The optimal parameters of aerial seeding includehigh speed, flight height of 2.5 m, flight speed of 4.0 m/s and operating distance of 6.0 m. Compared with the control group, plant height, ground diameter and above-ground biomass of the buckwheat infected with Dark Septate Endophytes(DSE)increased by 11%、25%、49%, respectively. In addition, the DSE infection could increase the SPADR and photosynthetic rate of buckwheat, promoting the root to grow and improving the yield and quality of buckwheat. This UAV aerial seeding combined with growth promotion of DSE infection could offer reference for ecological restoration of complex terrain in the western mining areas

Effect of Lactobacillus delbrueckii subsp. lactis on vaginal radiotherapy for gynecological cancer

Abstract The aim of this study was to evaluate the effect of Lactobacillus delbrueckii subsp. lactis (L.del) on vaginal microbiota (VM) dysbiosis and vaginal radiation injury in gynecologic cancer patients. The inhibitory effects of L.del on cervical cancer cells were also studied in vitro. Gynecologic cancer patients receiving radiotherapy were randomized into control and L.del intervention groups. The control group received radiotherapy, while the intervention group received radiotherapy and L.del intervention (1 capsule/day placed into the deep vagina from the first day of radiotherapy until the end of treatment). Vaginal swab samples were collected on the first day pre-treatment and the last day post-treatment. DNA from 54 patients was extracted and assessed by the 16S rRNA sequencing method. Radiotherapy resulted in vaginal microbiome dysbiosis characterized by increased phylogenetic diversity and increased abundance of Brevundimonas, Streptococcus and Prevotella, but a decreased abundance of Lactobacillus. Level 2 vaginal radiation injury was positively associated with the abundance of Brevundimonas and gram-negative non-fermenting bacteria. Administration of L.del attenuated the reduction of Lactobacillus while also inhibiting the abundance of Streptococcus and Prevotella, thereby ameliorating radiotherapy-related vaginal microbiota dysbiosis. CLD inhibited the in vitro proliferation of SiHa cells by altering the expression of BCL2, HPV16-E6, HPV16-E7, IL6, MAP7, BAX, Caspase-3, Caspase-9 and LTF. In conclusion, L. del application can alleviate radiation-induced vaginal dysbiosis and restore Lactobacillus dominance of the vaginal microbiome. Moreover, CLD was found to inhibit cell growth and promote the apoptosis of SiHa cells in vitro. The registration number for this clinical trial is ChiCTR1900021784