Improved ordinary measure and image entropy theory based intelligent copy detection method

Agency for Science, Technology and Research (A*STAR

Pseudotyped lentiviral vectors: Ready for translation into targeted cancer gene therapy?

Gene therapy holds great promise for curing cancer by editing the deleterious genes of tumor cells, but the lack of vector systems for efficient delivery of genetic material into specific tumor sites in vivo has limited its full therapeutic potential in cancer gene therapy. Over the past two decades, increasing studies have shown that lentiviral vectors (LVs) modified with different glycoproteins from a donating virus, a process referred to as pseudotyping, have altered tropism and display cell-type specificity in transduction, leading to selective tumor cell killing. This feature of LVs together with their ability to enable high efficient gene delivery in dividing and non-dividing mammalian cells in vivo make them to be attractive tools in future cancer gene therapy. This review is intended to summarize the status quo of some typical pseudotypings of LVs and their applications in basic anti-cancer studies across many malignancies. The opportunities of translating pseudotyped LVs into clinic use in cancer therapy have also been discussed

Asymmetric line edge roughness of multilayer grating reference materials

Line edge roughness (LER) in a one dimensional Si/SiO2 multilayer grating reference material with 20 nm nominal pitch size was investigated. It was shown for the first time that the LER of Si on SiO2 edges was about three times larger than that of SiO2 on Si edges. The asymmetric LERs led to asymmetric uncertainties and it must be considered when using the multilayer grating reference material. Moreover, the origin of asymmetric LER was investigated based on distinguishing contributions of interfacial roughness and interfacial diffusion. The interfacial roughness was determined to be symmetric because the surface roughness of the substrate, the Si single layer, the Si/SiO2 bilayer and the Si/SiO2 multilayer were almost the same with the value about 0.1 nm. Whereas, the interfacial diffusion layer of Si on SiO2 interface was about two times larger than that of SiO2 on Si interface through X-ray reflectivity (XRR) reverse fitting and transmission electron microscopy (TEM) image analysis. The asymmetric interfacial diffusion layer was proposed to be the main reason of the observed asymmetric LERs of the Si/SiO2 multilayer grating reference material

Impacts of Urbanization on River Systems in the Taihu Region, China

River systems are valuable to human beings; meanwhile, they are intensively influenced by human activities, especially urbanization. In this study, based on the data derived from topographic maps and remote sensing images, the temporal and spatial change of river system geomorphology in the Taihu Region over the past 50 years was investigated in conjunction with urbanization. Results demonstrated that the number of river systems decreased drastically, that the morphology of river channels changed into wider and straighter and that the structure of river network tended to simplify in the Taihu Region in recent 50 years. Meanwhile, the changes in river density, the water surface ratio, the river development coefficient, the main river area length ratio and the box dimension in the rapid urbanization period were much greater than those in the slow urbanization period, but the decrease of river sinuosity in the slow urbanization period was more intense. Moreover, the spatial differences of the changes in the river development coefficient were the largest, and the changes in the river indicators in the low-urbanized regions were the most intense. In addition, the changes in the water surface ratio had the closest correlation with urbanization, and the relational degrees between population urbanization and the changes in river systems were the largest. The results can provide a reliable basis to determine reasonable management and conservation strategies of river systems in the Taihu Region

Optimization of Nano-Grating Pitch Evaluation Method Based on Line Edge Roughness Analysis

Pitch uncertainty and line edge roughness are among the critical quality attributes of a pitch standard and normally the analyses of these two parameters are separate. The analysis of self-traceable Cr atom lithography nano-gratings shows a positive relevance and sensitivity between LER and evaluated standard deviation of pitch. Therefore, LER can be used as an aided pre-evaluation parameter for the pitch calculation method, such as the gravity center method or the zero-crossing points method. The optimization of the nano-grating evaluation method helps to obtain the accurate pitch value with fewer measurements and provide a comprehensive characterization of pitch standards

Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters

Abstract A total of 48 endophytic bacteria were isolated from surface-sterilized tissues of the medicinal plant Lonicera japonica, which is grown in eastern China; six strains were selected for further study based on their potential ability to promote plant growth in vitro (siderophore and indoleacetic acid production). The bacteria were characterized by phylogenetically analyzing their 16S rRNA gene similarity, by examining their effect on the mycelial development of pathogenic fungi, by testing their potential plant growth-promoting characteristics, and by measuring wheat growth parameters after inoculation. Results showed that the number of endophytic bacteria in L. japonica varied among different tissues, but it remained relatively stable in the same tissues from four different plantation locations. Among the three endophytic strains, strains 122 and 124 both had high siderophore production, with the latter showing the highest phosphate solubilization activity (45.6 mg/L) and aminocyclopropane-1-carboxylic acid deaminase activity (47.3 nmol/mg/h). Strain 170 had the highest indoleacetic acid (IAA) production (49.2 mg/L) and cellulase and pectinase activities. After inoculation, most of the six selected isolates showed a strong capacity to promote wheat growth. Compared with the controls, the increase in the shoot length, root length, fresh weight, dry weight, and chlorophyll content was most remarkable in wheat seedlings inoculated with strain 130. The positive correlation between enzyme (cellulose and pectinase) activity and inhibition rate on Fusarium oxysporum, the IAA production, and the root length of wheat seedlings inoculated with each tested endophytic strain was significant in regression analysis. Deformity of pathogenic fungal mycelia was observed under a microscope after the interaction with the endophytic isolates. Such deformity may be directly related to the production of hydrolytic bacterial enzymes (cellulose and pectinase). The six endophytic bacterial strains were identified to be Paenibacillus and Bacillus strains based on the results of 16S rRNA gene sequencing analysis and their physiological and biochemical characteristics. Results indicate the promising application of endophytic bacteria to the biological control of pathogenic fungi and the improvement of wheat crop growth