99 research outputs found
Percent of variation accounted for by Partial Least Squares (PLS) regression analysis of the parasitism rate.
<p>Percent of variation accounted for by Partial Least Squares (PLS) regression analysis of the parasitism rate.</p
Model output for predicted (landscape) and response (parasitism rate) variables used in the Partial Least Squares (PLS) regression analysis.
<p>Model output for predicted (landscape) and response (parasitism rate) variables used in the Partial Least Squares (PLS) regression analysis.</p
Landscape diversity index (Simpson’s <i>D</i>) and the proportion of other landscape variables at four spatial scales across all 33 sites.
<p>Landscape diversity index (Simpson’s <i>D</i>) and the proportion of other landscape variables at four spatial scales across all 33 sites.</p
Correlation loadings for the plot of the response variable (parasitism rate, Y) and predictor variables across four spatial scales based on PLS analysis.
<p>Radius: (a) 0.5 km; (b) 1.0 km; (c) 1.5 km; (d) 2.0 km. The four dotted line circles (25%, 50%, 75% and 100%) indicate the percentage range of variation explained by the first two factors. The distance of marked points from the origin and from the horizontal and vertical axes jointly show the amount of variation in each variable accounted for by the first two factors.</p
Generation and Application of a Novel InsP<sub>3</sub>R<sub>1</sub> Mono-Antibody from Mouse
<div><p>Inositol 1, 4, 5-Trisphosphate Receptor (InsP<sub>3</sub>R) is an intracellular Ca<sup>2+</sup> release channel, which widely participates in cellular processes. Three isoforms of InsP<sub>3</sub>R were identified as InsP<sub>3</sub>R<sub>1</sub>, InsP<sub>3</sub>R<sub>2,</sub> and InsP<sub>3</sub>R<sub>3</sub>. They share 60–0% protein sequence homology and form a channel in a manner of homotetramer or heterotetramer. Several InsP<sub>3</sub>R isoform-specific rabbit antibodies have been generated to distinguish their localization and functions. However, there is no report of such a valid antibody raised from other species. In his article, we prepare a mouse monoclonal antibody against a synthetic peptide with rat InsP<sub>3</sub>R<sub>1</sub>-specific carboxyl terminus sequence. This monoclonal antibody of InsP<sub>3</sub>R<sub>1</sub> (R1-mAb) was purified and characterized as IgG2b, which can recognize InsP<sub>3</sub>R<sub>1</sub> by Western-blot (WB) analysis and immunoprecipitate (IP) InsP<sub>3</sub>R<sub>1</sub> from moue brain lysate tested. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody could give the consistent results in SH-SY5Y cells, human sperm, and mouse brain paraffin section. In summary, we generate a mouse InsP<sub>3</sub>R<sub>1</sub>-specific IgG 2b antibody identifying InsP<sub>3</sub>R<sub>1</sub> in WB, IF, IHC, and IP analysis, which provides a possible choice for detection of InsP<sub>3</sub>R<sub>1</sub>, especially in application of co-localization analysis with other InsP<sub>3</sub>R isoforms or other proteins.
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Vulnerability evaluation indicator system for urban agglomerations along the Yellow River.
Vulnerability evaluation indicator system for urban agglomerations along the Yellow River.</p
Obstacle factors and obstacle degree in 2001 and 2020 for the urban ag-glomerations along the Yellow River.
Obstacle factors and obstacle degree in 2001 and 2020 for the urban ag-glomerations along the Yellow River.</p
Analysis of spatial variability within the urban agglomerations along the Yellow River, 2001–2005, 2006–2010, 2011–2015 and 2016–2020.
Analysis of spatial variability within the urban agglomerations along the Yellow River, 2001–2005, 2006–2010, 2011–2015 and 2016–2020.</p
Relationship between RGR of height and leaf [N], [P] and N∶P ratio for the transplanted <i>C. chinensis</i>, <i>M. chapensis</i>, and <i>P. rubra</i> seedlings.
<p>Relationship between RGR of height and leaf [N], [P] and N∶P ratio for the transplanted <i>C. chinensis</i>, <i>M. chapensis</i>, and <i>P. rubra</i> seedlings.</p
Relative growth rate (RGR) of height for transplanted <i>C. chinensis</i>, <i>M. chapensis</i>, and <i>P. rubra</i> seedlings affected by removing (VR) or retaining (CK) understory vegetation and litter at the two plantations.
<p>EP: <i>Eucalyptus</i> plantation; NP: native species plantation.</p
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