Identification and Characterization of Agv1, a Pre-Metazoan Arf GAP: A Dissertation

Human immunodeficiency virus type 1 (HIV-1) is a member of the lentivirus subfamily of retroviruses. HIV-1 expresses multiple genes from a single provirus by alternative splicing. Early in viral expression, fully spliced 2-kb viral RNA is exported from the nucleus and encodes the viral regulatory protein, Rev, which is essential for nuclear transport of partially spliced and unspliced genomic-length RNA. Rev binds to an RNA structural element called the Rev response element (RRE) and mediates nuclear export through the leucine-rich nuclear export signal (NES) pathway. The human Rev Interacting Protein (hRIP) interacts specifically with the Rev NES. Rev NES mutants that are unable to export Rev-dependent RNAs are also unable to bind to hRIP. The hRIP cDNA encodes a 562 amino acid protein containing an N-terminal zinc finger with homology to Arf GAP domains, a central serine and threonine rich region, and C-terminal phenylalanine-glycine (FG) repeats characteristic of nucleoporins. To identify an hRIP ortholog in a genetically tractable organism, we performed database searches using the N-terminal zinc finger of hRIP. Using this approach, we identified a novel gene in Schizosaccharomyces pombe. Alignment of the entire reading frame of the putative ortholog with hRIP indicates similarity with the serine/threonine rich region and with the FG repeats, suggesting that S. pombecould be a good model system to study the cellular function of hRIP. We find that the S. pombe ORF is an essential gene, which encodes a 483 amino acid protein that is also able to interact with the NES of HIV-1 Rev. Based on being an essential gene, and the presence of a putative Arf GAP domain, the ORF was named an Arf GAP essential for viability, agv1+. We show that Agv1 is not directly involved in the nuclear export of poly(A+) RNA or 5S rRNA, nuclear export of leucine-rich NES-containing proteins, or nuclear import of nuclear localization signal (NLS)-containing proteins. However, Agv1 does appear to play a role in the cytoplasmic localization of 5S rRNA. We demonstrate that loss of Agv1 alters the localization of endoplasmic reticulum (ER) membrane and Golgi membrane resident proteins, accumulates intracellular membrane, and blocks processing of carboxypeptidase Y. Furthermore, the S. cerevisiae ADP-ribosylation factor (Arf) GTPase activating protein (GAP) Glo3, but not a catalytically inactive Glo3 mutant [R59K], is able to partially compensate for the loss of Agv1 function in temperature sensitive strains, indicating that Agv1 is an S. pombe Arf GAP with some functional features similar to S. cerevisiae Glo3

The Neglect of Quantitative Research in Green Criminology and Its Consequences

While interest in green criminology has rapidly expanded over the past twenty-five years, much of this growth has occurred on the periphery of orthodox criminology. This article suggests that green criminology’s marginalization is partially a result of its non-quantitative methodology. We hypothesize that non-quantitative tendencies within green criminology distance it from orthodox criminology because orthodox criminology values quantitative methods (Tewksbury et al. in J Crim Justice Educ 16(2):265–279, 2005). Here, we examine how neglecting quantitative research methods may contribute to inattention to green criminology within orthodox criminology, and we consider what can be done to change that situation. We suggest that employing quantitative approaches within green criminology is one way to increase its appeal to mainstream criminology, and that quantitative studies, in conjunction with other research methodologies, can also enhance generalizability of findings, influence policy, and advance theory construction and hypothesis testing

Does the modernization of environmental enforcement reduce toxic releases? An examination of self-policing, criminal prosecutions and toxic releases in the United States, 1988–2014

According to modernization theory, enforcement schemes that rely on end-of-the-pipe regulation are not as effective at achieving improved environmental performance as market-based approaches that encourage pollution prevention. Consistent with that observation, the U.S. Environmental Protection Agency transitioned to the use of self-policing to encourage pollution prevention. Other studies note that environmental compliance is significantly affected by traditional “command-and-control” strategies. Using Prais Winston regression we examine these contrasting views by estimating the relationship between toxic releases, self-policing, and criminal prosecutions from 1988 through 2014. Initial correlations suggest that (1) self-policing is not associated with toxic releases but that (2) criminal prosecutions may reduce toxic releases through general deterrence signals. Subsequent analyses controlling for gross domestic product revealed that neither self-policing nor criminal enforcement correlate with toxic releases but that gross domestic product was the strongest predictor of emissions. The implications of these findings for the control of toxic emissions are discussed

From Structural Inequalities to Speaking Out: Youth Participatory Action Research in College Access Collaborations

In recent years, participatory action research projects aimed at addressing local social issues have gained interest in academic settings. These projects can contribute to university-community partnerships, but communication about such projects remains somewhat limited. This article contributes to these developing discussions by describing how a youth participatory action research project (YPAR) supported an ongoing university-community partnership between South University, a mid-sized private liberal arts school, and the local public school community. This educational partnership led to the Anchor Academy, a college access and success program for high school youth with limited financial resources and little or no family history of college. In 2010-2011, Anchor initiated a YPAR project to study the challenges limited-income, first-generation, and minority students faced on their path to college. This article describes how the project deepened university-community relationships, shaped broader awareness and local programming, and inspired a ripple effect of new partnerships that help to sustain the work of supporting marginalized students in their journeys toward college futures. It addresses the struggles faced by the project as well as the positive outcomes, ultimately arguing for the potential for critical, participatory research methodologies to serve as a particularly meaningful platform for collaboration between universities and their communities

Job Coaching: A Means to Reduce Unemployment and Underemployment in the Deaf Community

Historically, vocational rehabilitation approaches to assisting deaf people to succeed in employment have been lacking. This contributes to unemployment and underemployment among the deaf community. This article discusses barriers to employment experienced by deaf people, the traditional role of vocational rehabilitation services, and service gaps related to job retention and advancement. A model for the provision of job coach services is provided that calls for the coach to be a member of a team providing routine ongoing services. Current and future efforts to develop coach training opportunities are described as well

Receipt of Guideline-Concordant Care Among Older Women With Stage I-III Breast Cancer: A Population-Based Study

Background: This study examined receipt of guideline-concordant care (GCC) according to evidence-based treatment guidelines and quality measures and specific types of treatment among older women with breast cancer. Patients and Methods: A total of 142,433 patients aged ≥66 years diagnosed with stage I–III breast cancer between 2007 and 2011 were identified in the SEER-Medicare linked database. Algorithms considering cancer characteristics and the appropriate course of care as per guidelines versus actual care received determined receipt of GCC. Multivariable logistic regression estimated the likelihood of GCC and specific types of treatment for women aged ≥75 versus 66 to 74 years. Results: Overall, 39.7% of patients received GCC. Patients diagnosed at stage II or III, with certain preexisting conditions, and of nonwhite race were less likely to receive GCC. Patients with hormone-negative tumors, higher grade tumors, and greater access to oncology care resources were more likely to receive GCC. Patients aged ≥75 years were approximately 40% less likely to receive GCC or adjuvant endocrine therapy, 78% less likely to have any surgery, 61% less likely to have chemotherapy, and about half as likely to have radiation therapy than those aged 66 to 74 years. Conclusions: Fewer than half of older women with breast cancer received GCC, with the lowest rates observed among the oldest age groups, racial/ethnic minorities, and women with later-stage cancers. However, patients with more aggressive tumor characteristics and greater access to oncology resources were more likely to receive GCC. Considering that older women have the highest incidence of breast cancer and that many are diagnosed at stages requiring more aggressive treatment, efforts to increase rates of earlier stage diagnosis and the development of less toxic treatments could help improve GCC and survival while preserving quality of life

Mindful Persistence: Literacies for Taking up and Sustaining Fermented-Food Projects

Almost by definition, resisting the insidious convenience of the mainstream food supply requires persistence. This is especially true for food projects requiring fermentation—projects that unfold over days or weeks and require day-to-day science in kitchens where variables can be hard to control and where some degree of periodic failure is almost inevitable. In this article, a team of writers—scholars and community members—dramatizes a joint inquiry from which emerged a composite portrait of what we have come to call mindful persistence—an existential yet collaborative engine that drives our food literacies. Dialogic text features highlight the situated insights of individual writers, indicating that while this team shares an interest in fermentation, this interest does not require or assume identical understandings of the science of fermentation or similar positions in the probiotic debate surrounding contemporary fermentation practices. Instead, what is shared is a mindful persistence that scaffolds reflective action in this dynamic problem space