2,345 research outputs found
Poly(amido amine)s as Gene Delivery Vectors: Effects of Quaternary Nicotinamide Moieties in the Side Chains
To evaluate the effect of quaternary nicotinamide pendant groups on gene delivery properties, a series of poly(amido amine) (co)polymers were synthesized by Michael addition polymerization of N, N-cystaminebisacrylamide with variable ratios of 1-(4-aminobutyl)-3-carbamoylpyridinium (Nic-BuNH2), and tert-butyl-4-aminobutyl carbamate (BocNH-BuNH2), yielding poly(amido amine)s (NicX-NHBoc) with X=0, 10, 30, and 50 % of quaternary nicotinamide groups in the polymer side chains. Deprotection of the pendant Boc-NH groups afforded an analogous series of polymers (NicX-NH2) with higher charge density (due to the presence of protonated primary amino groups in the side chains) and subsequent acetylation yielded a series of polymers (NicX-NHAc) of lower hydrophobicity than the Boc-protected polymers. The polymers with the Boc-protected or the acetylated amino groups showed high buffer capacity in the range pH 5.1-7.4, which is a property that can contribute to endosomal escape of polyplexes. The presence of quaternary nicotinamide groups has distinct beneficial effects on the gene vector properties of these polymers. The polymers containing 30 % of quaternary nicotinamide groups in their side chains condense DNA into small, nanosized particles (200 nm) with positive surface charge (+15 mV). Fluorescence experiments using ethidium bromide as a competitor showed that the quaternary nicotinamide groups intercalate with DNA, contributing to a more intimate polymer-DNA binding and shielding. Polyplexes of nicotinamide-functionalized poly(amido amine)s NicX-NHBoc and NicX-NHAc, formed at 12/1 polymerDNA mass ratio, efficiently transfect COS-7 cells with efficacies up to four times higher than that of PEI (Exgen 500), and with essentially absence of cytotoxicity. NicX-NH2 polymers, possessing protonated primary amino groups in their side chains, have a higher cytotoxicity profile under these conditions, but at lower 3/1 polymer-DNA mass ratio also these polymers are capable of efficient transfection, while retaining full cell viability
Electron spin resonance on a 2-dimensional electron gas in a single AlAs quantum well
Direct electron spin resonance (ESR) on a high mobility two dimensional
electron gas in a single AlAs quantum well reveals an electronic -factor of
1.991 at 9.35 GHz and 1.989 at 34 GHz with a minimum linewidth of 7 Gauss. The
ESR amplitude and its temperature dependence suggest that the signal originates
from the effective magnetic field caused by the spin orbit-interaction and a
modulation of the electron wavevector caused by the microwave electric field.
This contrasts markedly to conventional ESR that detects through the microwave
magnetic field.Comment: 4 pages, 4 figure
Reply:
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87032/1/24410_ftp.pd
Remote Hardware-In-the-Loop Measurement System for Electrolyser Characterization
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The installation of facilities replicating the realworld condition is often required for carrying out meaningful tests on new devices and for collecting data with the aim to create realistic device model. However, these facilities require huge investments, as well as areas where they can be properly installed. In this paper, we present a test infrastructure exploiting the concept of Remote Power Hardware-In-the-Loop (RPHIL), applied for characterizing the performances of a 8kW Proton Exchange Membrane (PEM) electrolyser installed at the Hanze University of Applied Sciences in Groningen (The Netherlands). The electrolyser is subjected to different test conditions imposed both locally and remotely. The results show that this measurement procedure is effective and can open new perspectives in the way to share and exploit the existing research infrastructure in Europe
Soil‐Plant Indicators for Determining the Impact of Management on the Stability of Grasslands in Cattle Exploitation
A new mechanism for exchange processes observed in the compounds [M(η-C_5H_5)_2(exo-η-RCH = CH_2)H], M = Nb and Ta
Dynamic n.m.r. studies of the exchange processes in the complexes [M(η-C_5H_5)(exo-η-RCH=CH_2)H], M = Nb, Ta, lead to the proposal of a new mechanism involving intermediates with agostic bonding
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Embedded Agency in Institutional Theory: Problem or Paradox
In “Beyond Constraining and Enabling: Toward New Microfoundations in Institutional Theory” Professor Cardinale (2018) seeks to expose and correct “shortcomings” (p.133) in institutional theory’s conceptualization of structure, agency and their relationship. To this end, he theorizes the “different mechanism[s] through which actors are embedded in structure” (p.134). We agree that institutional theory’s microfoundations merit theoretical attention and development. However, we question the premise that the issue of agency in institutional theory is adequately, or even plausibly, formulated as one of “embeddedness”. We also challenge the relevance of Professor Cardinale’s engagement of Husserl to help solve what we argue to be a phantom problem central to his theory
Exciton condensate at a total filling factor of 1 in Corbino 2D electron bilayers
Magneto-transport and drag measurements on a quasi-Corbino 2D electron
bilayer at the systems total filling factor 1 (v_tot=1) reveal a drag voltage
that is equal in magnitude to the drive voltage as soon as the two layers begin
to form the expected v_tot=1 exciton condensate. The identity of both voltages
remains present even at elevated temperatures of 0.25 K. The conductance in the
current carrying layer vanishes only in the limit of strong coupling between
the two layers and at T->0 K which suggests the presence of an excitonic
circular current
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