Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru

Heliotropium arborescens (Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the in vitro antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three in vitro methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values ​​obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC50 37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R2 0.9989), TFC/ABTS (r = 0.9572; R2 0.9998) and TFC/FRAP (r = 09933; R2 0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of H. arborescens L. show potential antioxidant activity, and moderate antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Graphical abstract

Abamectin and emamectin in grapes of Vitis vinifera L. from a district of the Valley of Ica-Peru

Context: In 14 districts of the valley of Ica-Peru, Vitis vinifera L. plants are cultivated that produce grapes for consumption as table grapes and raisins (dried grapes); at the same time, for the production of wines and Piscos. Aims: To determine the levels of abamectin and emamectin in grapes of Vitis vinifera L from a district of the Valley of Ica-Peru. Methods: 30 lots (30 kg) of Moscatel grape variety V. vinifera L. were collected from six countryside (artisanal and organic cultivation) of the San Juan Bautista district. The extraction of abamectin (ABM) and emamectin benzoate (EMB) from the grapes was carried out with acetonitrile; it was quantified by means of Liquid Chromatography coupled to Mass Spectrometry (HPLC-MS). The maximum permissible limit values (MRL) were established at 0.010 ppm for both insecticides. Results: The determined levels of abamectin and emamectin in grapes were 0.0012-0.015 ppm and 0.0013-0.013 ppm, respectively. Values higher than the maximum permissible limits of abamectin were found in batches A2 (0.0102 ppm), C1 (0.015 ppm), C5 (0.0113 ppm), and F2 (0.012 ppm); emamectin benzoate in lots B1 (0.0113 ppm), B4 (0.013 ppm) and C4 (0.012 ppm). Using the Shapiro-Wilk, Anderson Darling and Student’s t tests, it was found that the global means of the values of the two insecticides in grapes are lower than the MRL. According to the global analysis of variance, the means of the concentrations of both insecticides were not different between the six sampling zones (countryside). Conclusions: The insecticides abamectin and emamectin are below the maximum permissible limit values (0.010 ppm) in Moscatel grapes of Vitis vinifera L., so the residual effect would not have implications for human health

Assessing parametric uncertainty on fibre reinforced composite laminates

Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2016 do Instituto Politécnico de Lisboa. Código de referência IPL/2016/CompDrill_ISELWhen modeling a composite structure it is important to take into account its greater exposition to parametric variability, when compared to other types of materials more traditionally used. The possibility to tailor composite materials according to specific requisites is simultaneously a source of additional variability, which origin may be associated to material and to geometrical characteristics. Regardless the origin of this variability, they will produce its effect in the structure response, thus it is very important to anticipate them and to quantify them as much as possible. With the present work, it is intended to assess and quantify the influence of geometrical parameters variabilities on the composite structural response. Behind this characterization, linear static analyses were performed, considering that the layers’ thicknesses and fibre orientation angles will be affected by uncertainty. A set of simulations and numerical results are presented and discussed.info:eu-repo/semantics/publishedVersio

Deficiencias, discapacidades y factores desencadenantes, en la parroquia Bellavista de la ciudad de Cuenca

El proyecto se enmarca en las líneas de acción de la Comisión de Extensión Universitaria, se aborda un problema de salud pública involucrando a estudiantes de tres Escuelas de la Facultad de Ciencias Médicas, quienes trabajaron en grupos de dos, tres y cuatro personas por parroquia, investigando las deficiencias, discapacidades y sus factores desencadenantes en las familias del área urbana de Cuenca, Los habitantes fueron 25364, constituidos en 5072 familias, cada familia con un promedio de 5 miembros. La muestra fue de 275 familias entrevistadas, de 1175 personas, el promedio por familia de 4.27 miembros. El número mínimo de personas por casa visitadada fue de 1 y el máximo de 8. Conclusiones en anexoLicenciado en EnfermeríaCuenc

A study on the structural behaviour of FGM plates static and free vibrations analyses

Functionally graded materials are characterised by a determined spatial composition variation of their phases’ constituents, which enable for a closer suitability of the material properties to the desired mechanical behaviour. Concerning to the engineered construction of these materials, they can be thought as being achieved by considering a continuous variation of their phases and thus of their properties, or by considering a discrete stacking of a sufficient number of layers, in order to ensure a less abrupt variation profile of their properties. Also, depending on the nature of the applications, it may be important to consider a sandwich configuration, where the three-layered constitution may correspond to a functional requisite. With the present work, these two situations will be studied, considering different methodologies based either on a meshless method or on different approaches based on the finite element method. A comparative study of the performance and adequacy of the developed models is carried out through a set of illustrative cases focused on the study of static and free vibrations behaviour of plate structures.info:eu-repo/semantics/publishedVersio

A study on the structural behaviour of FGM plates static and free vibrations analyses

Functionally graded materials are characterised by a determined spatial composition variation of their phases’ constituents, which enable for a closer suitability of the material properties to the desired mechanical behaviour. Concerning to the engineered construction of these materials, they can be thought as being achieved by considering a continuous variation of their phases and thus of their properties, or by considering a discrete stacking of a sufficient number of layers, in order to ensure a less abrupt variation profile of their properties. Also, depending on the nature of the applications, it may be important to consider a sandwich configuration, where the three-layered constitution may correspond to a functional requisite. With the present work, these two situations will be studied, considering different methodologies based either on a meshless method or on different approaches based on the finite element method. A comparative study of the performance and adequacy of the developed models is carried out through a set of illustrative cases focused on the study of static and free vibrations behaviour of plate structures.info:eu-repo/semantics/publishedVersio

A comprehensive review on analysis of nanocomposites: from manufacturing to properties characterization

The study of nanocomposites in its diverse scientific fields has increased dramatically over the years with numerous theoretical and experimental techniques emerging and redefining the process of synthesis, analysis and cost control methodologies of nanocomposites. The present review is an attempt to identify the various methodologies, techniques, theories and formulations that are used in nanocomposite technology. As an overall qualitative appreciation it is possible to conclude that the diversity of processes involved in the manufacture and analysis of nanocomposites, impacts them differently, influencing their physical nature, chemical behaviour, biological interactions, optical properties and production costs which consequently may introduce some constraints to their application. Hence, a critical review on the best methodology would remain inconclusive. This work intends to collect and relate publications on different fields of the nanocomposites technology and application fields, aiming at contributing to achieve a wide perspective of different aspects of the nanocomposites processes and theories and with this, being an aid to ease and raise the production and analysis of nanocomposites to a higher level

Influence of interphase on the multi-physics coupled frequency of three-phase smart magneto-electro-elastic composite plates

The present article researches the influence of piezoelectric interphase thickness on the coupled frequency response of three-phase smart magneto-electro-elastic (TPS-MEE) plates with the aid of Reddy's third-order shear deformation theory (RTSDT). A three-phase smart composite constituted of CoFe2O4 piezomagnetic matrix embedded with carbon fibers in the piezoelectric shell is considered for evaluation. The coupling characteristics of the smart carbon/PZT-5A (PZT-7A)/CoFe2O4 composites significantly changes with the interphase thickness of piezoelectric interphase. Thereby the stiffness and the natural frequency of the structure composed of these three-phase MEE materials drastically changes. A finite element (FE) formulation has been derived incorporating Hamilton's principle. The equations of motion are obtained through condensation technique. The results reveal a significant effect of interphase thickness on the natural frequency of the three-phase smart magneto-electroelastic plate. Further, a special attention has been paid on evaluating the influence of carbon fiber/piezoelectric volume fraction on the free vibration behaviour of TPS-MEE plate. Also, a parametric study has been performed to investigate the effect of boundary conditions, aspect ratio and length-to-width ratio

Impaired mitophagy in Fanconi anemia is dependent on mitochondrial fission

10.18632/oncotarget.11161Oncotarget73658065-5807