Un capteur à alcool (gaz) pour l'interface Orphy

Partie 2 (p. 117-125) : http://www.epi.asso.fr/revue/82som.htm#b82p117Sommaires des numéros :http://archive-edutice.ccsd.cnrs.fr/edutice-00000869http://archive-edutice.ccsd.cnrs.fr/edutice-000008701re partie : Principe de fonctionnement et étude de la réponse [b81p119]On cherche à : - établir la relation entre la tension produite par ce capteur - non linéaire - et la concentration des solutions alcooliques avec lesquelles il est en contact par la phase vapeur, - en évaluer les limite de détection et sensibilité, dans le but d'utilisations quantitatives telles que le dosage de solutions alcooliques diluées et le suivi en continu de fermentations. 2e partie : Utilisation pour le suivi de fermentations [b82p117]L'étude de la réponse de ce capteur à gaz à des solutions d'alcool a conduit au protocole d'étalonnage pour des utilisations quantitatives . Nous l'appliquons pour suivre la fermentation du glucose par les levures et étudier l'effet de la concentration du sucre. Le dosage de l'alcool dans le milieu permettra d'apprécier la réponse du capteur dans ces conditions d'expérience

Protein Kinase D1 (PKD1) Is a New Functional Non-Genomic Target of Bisphenol A in Breast Cancer Cells

International audienceExposure to bisphenol A (BPA), one of the most widespread endocrine disruptors present in our environment, has been associated with the recent increased prevalence and severity of several diseases such as diabetes, obesity, autism, reproductive and neurological defects, oral diseases, and cancers such as breast tumors. BPA is suspected to act through genomic and non-genomic pathways. However, its precise molecular mechanisms are still largely unknown. Our goal was to identify and characterize a new molecular target of BPA in breast cancer cells in order to better understand how this compound may affect breast tumor growth and development. By using in vitro (MCF-7, T47D, Hs578t, and MDA-MB231 cell lines) and in vivo models, we demonstrated that PKD1 is a functional non-genomic target of BPA. PKD1 specifically mediates BPAinduced cell proliferation, clonogenicity, and anchorage-independent growth of breast tumor cells. Additionally, low-doses of BPA (≤10 −8 M) induced the phosphorylation of PKD1, a key signature of its activation state. Moreover, PKD1 overexpression increased the growth of BPA-exposed breast tumor xenografts in vivo in athymic female Swiss nude (Foxn1 nu/nu) mice. These findings further our understanding of the molecular mechanisms of BPA. By defining PKD1 as a functional target of BPA in breast cancer cell proliferation and tumor development, they provide new insights into the pathogenesis related to the exposure to BPA and other endocrine disruptors acting similarly