Laboratory measurements of the sound generated by breaking waves

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution December 1991Breaking waves dissipate energy, transfer momentum from the wind to surface currents and breaking enhances the transfer of gas and mass across the air-sea interface. Breaking waves are believed to be the dominant source of sea surface sound at frequencies greater than 500 Hz and the presence of breaking waves on the ocean surface has been shown to enhance the scattering of microwave radiation. Previous studies have shown that breaking waves can be detected by measuring the microwave backscatter and acoustic radiation from breaking waves. However, these techniques have not yet proven effective for studying the dynamics of breaking. The primary motivation for the research presented in this thesis was to determine whether measurements of the sound generated by breaking waves could be used to quantitatively study the dynamics of the breaking process. Laboratory measurements of the microwave backscatter and acoustic radiation from two-dimensional breaking waves are described in Chapter 2. The major findings of this Chapter are: 1) the mean square acoustic pressure and backscattered microwave power correlate with the wave slope and dissipation for waves of moderate slope, 2) the mean square acoustic pressure and backscattered microwave power correlate strongly with each other, and 3) the amount of acoustic energy radiated by an individual breaking event scaled with the amount of mechanical energy dissipated by breaking. The observed correlations with the mean square acoustic pressure are only relevant for frequencies greater than 2200 Hz because lower frequencies were below the first acoustic cut-off frequency of the wave channel. In order to study the lower frequency sound generated by breaking waves another series of two-dimensional breaking experiments was conducted. Sound at frequencies as low as 20 Hz was observed and the mean square acoustic pressure in the frequency band from 20 Hz-l kHz correlated strongly with the wave slope and dissipation. A characteristic low frequency signal was observed immediately following the impact of the plunging wave crest. The origin of this low frequency signal was found to be the pulsating cylinders of air which are entrained by the plunging waves. The pulsation frequency correlated with both the wave slope and dissipation. Following the characteristic constant frequency signal, approximately 0.25 s after the initial impact of the plunging crest, another low frequency signal was typically observed. These signals were generally lower in frequency initially and then increased in frequency as time progressed. To determine if three-dimensional effects were important in the sound generation process and to measure the sound beneath larger breaking waves a series of experiments was conducted in a large multi-paddle wave basin. Three-dimensional breaking waves were generated and the sound produced by breaking was measured in the frequency range from 10 Hz to 20 kHz. The observed sound spectra showed significant increases in level across the entire bandwidth from 10 Hz to 20 kHz and the spectra sloped at -5 to-6 dB per octave at frequencies greater than 1 kHz. The mean square acoustic pressure in the frequency band from 10 Hz to 150 Hz correlated with the wave amplitude similar to the results obtained in the two-dimensional breaking experiments. Large amplitude low frequency spectral peaks were observed approximately 0.75 s after the initial impact of the plunging crests. It was postulated that the low frequency signals observed some time after the initial impact of the plunging crests for both the two and three-dimensional breakers were caused by the collective oscillation of bubble clouds. Void fraction measurements taken by Eric Lamarre were available for five breaking events and therefore the average sound speed inside the bubble clouds and their radii were known. Using this information the resonant frequencies of a two-dimensional cylindrical bubble cloud of equal radius and sound speed were calculated. The frequencies of the observed signals matched closely with the calculated resonant frequencies of the first and second mode of the two-dimensional cylindrical bubble cloud. The close agreement supports the hypothesis that the low frequency signals were produced by the collective oscillation of bubble clouds. In Chapter 4 a model of the sound produced by breaking waves is presented which uses the sound radiated by a single bubble oscillating at its linear resonant frequency and the bubble size distribution to estimate the sound spectrum. The model generates a damped sinusiodal pulse for every bubble formed, as calculated from the bubble size distribution. If the range to the receiver is known then the only unknown parameters are ε, the initial fractional amplitude of the bubble oscillation and L, the dipole moment arm or twice the depth of the bubble below the free surface. It was found that if the product εxL is independent of the bubble radius the model reproduces the shape and magnitude of the observed sound spectrum accurately. The success of the model implies that it may be possible to calculate the bubble size distribution from the sound spectrum. The model was validated using data from experiments where the breaking events were small scale gently spilling waves (Medwin and Daniel, 1990)

Interrelationship between nutrients and chlorophyll-a in an urban stormwater lake during the ice-covered period

Urban stormwater lakes in cold regions are ice-covered for substantial parts of the winter. It has long been considered that the ice-covered period is the “dormant season,” during which ecological processes are inactive. However, little is known about this period due to the historical focus on the open-water season. Recent pioneering research on ice-covered natural lakes has suggested that some critical ecological processes play out on the ice. The objective of this study was to investigate the active processes in ice-covered stormwater lakes. Data collected during a two-year field measurement program at a stormwater lake located in Edmonton, Alberta, Canada were analyzed. The lake was covered by ice from November to mid-April of the following year. The mean value of chlorophyll-a during the ice-covered period was 22.09% of the mean value for the open-water season, suggesting that primary productivity under the ice can be important. Nitrogen and phosphorus were remarkably higher during the ice-covered period, while dissolved organic carbon showed little seasonal variation. Under ice-covered conditions, the total phosphorus was the major nutrient controlling the ratio of total nitrogen to total phosphorus, and a significant positive correlation existed between total phosphorus and chlorophyll-a when the ratio was smaller than 10. The results provide preliminary evidence of the critical nutrient processes in Stormwater Lake during the ice-covered period

Interrelationship Between Nutrients and Chlorophyll-a in an Urban Stormwater Lake During the Ice-covered Period

Urban stormwater lakes in cold regions are ice-covered for substantial parts of the winter. It has long been considered that the ice-covered period is the “dormant season,” during which ecological processes are inactive. However, little is known about this period due to the historical focus on the open-water season. Recent pioneering research on ice-covered natural lakes has suggested that some critical ecological processes play out on the ice. The objective of this study was to investigate the active processes in ice-covered stormwater lakes. Data collected during a two-year field measurement program at a stormwater lake located in Edmonton, Alberta, Canada were analyzed. The lake was covered by ice from November to mid-April of the following year. The mean value of chlorophyll-a during the ice-covered period was 22.09% of the mean value for the open-water season, suggesting that primary productivity under ice can be important. Nitrogen and phosphorus were remarkably higher during the ice-covered period, while dissolved organic carbon showed little seasonal variation. Under ice-covered conditions, the total phosphorus was the major nutrient controlling the ratio of total nitrogen to total phosphorus, and a significant positive correlation existed between total phosphorus and chlorophyll-a when the ratio was smaller than 10. The results provide preliminary evidence of the critical nutrient processes in the Stormwater Lake during the ice-covered period

A Phase I study of an intravesically administered immunotoxin targeting EpCAM for the treatment of nonmuscle-invasive bladder cancer in BCGrefractory and BCG-intolerant patients

Mark Kowalski1, Joycelyn Entwistle2, Jeannick Cizeau2, Demi Niforos1, Shauna Loewen2, Wendy Chapman1, Glen C MacDonald21Viventia Biotechnologies Inc., Mississauga, ON, Canada; 2Viventia Biotechnologies Inc., Winnipeg, MB, CanadaPurpose: A Phase I dose-escalation study was performed to determine the maximum tolerated dose (MTD) of the immunotoxin VB4-845 in patients with nonmuscle-invasive bladder cancer (NMIBC) refractory to or intolerant of bacillus Calmette–Guerin (BCG). Secondary objectives included evaluation of the safety, tolerability, pharmacokinetics, immunogenicity, and efficacy of VB4-845.Patients and methods: Sixty-four patients with Grade 2 or 3, stage Ta or T1 transitional cell carcinoma or in situ carcinoma, either refractory to or intolerant of BCG therapy, were enrolled. Treatment was administered in ascending dose cohorts ranging from 0.1 to 30.16 mg. After receiving weekly instillations of VB4-845 to the bladder via catheter for 6 consecutive weeks, patients were followed for 4–6 weeks post-therapy and assessed at week 12.Results: An MTD was not determined, as a dose-limiting toxicity was not identified over the dose range tested. VB4-845 therapy was safe and well tolerated with most adverse events reported as mild; as a result, no patients were removed from the study in response to toxicity. By the end of the study, the majority of patients had developed antibodies to the exotoxin portion of VB4-845. A complete response was achieved in 39% of patients at the 12-week time point.Conclusions: VB4-845 dosed on a weekly basis for 6 weeks was very well tolerated at all dose levels. Although an MTD was not determined at the doses administered, VB4-845 showed evidence of an antitumor effect that warrants further clinical investigation for the treatment of NMIBC in this patient population.Keywords: Pseudomonas exotoxin A, anti-EpCAM, fusion protein, targeted therap

Statistics of surface divergence and their relation to air-water gas transfer velocity

Air-sea gas fluxes are generally defined in terms of the air/water concentration difference of the gas and the gas transfer velocity,kL. Because it is difficult to measure kLin the ocean, it is often parameterized using more easily measured physical properties. Surface divergence theory suggests that infrared (IR) images of the water surface, which contain information concerning the movement of water very near the air-water interface, might be used to estimatekL. Therefore, a series of experiments testing whether IR imagery could provide a convenient means for estimating the surface divergence applicable to air-sea exchange were conducted in a synthetic jet array tank embedded in a wind tunnel. Gas transfer velocities were measured as a function of wind stress and mechanically generated turbulence; laser-induced fluorescence was used to measure the concentration of carbon dioxide in the top 300 μm of the water surface; IR imagery was used to measure the spatial and temporal distribution of the aqueous skin temperature; and particle image velocimetry was used to measure turbulence at a depth of 1 cm below the air-water interface. It is shown that an estimate of the surface divergence for both wind-shear driven turbulence and mechanically generated turbulence can be derived from the surface skin temperature. The estimates derived from the IR images are compared to velocity field divergences measured by the PIV and to independent estimates of the divergence made using the laser-induced fluorescence data. Divergence is shown to scale withkLvalues measured using gaseous tracers as predicted by conceptual models for both wind-driven and mechanically generated turbulence

Morphology and distribution of scales, dermal ossifications, and other non-feather integumentary structures in non-avialan theropod dinosaurs

Modern birds are typified by the presence of feathers, complex evolutionary innovations that were already widespread in the group of theropod dinosaurs (Maniraptoriformes) that include crown Aves. Squamous or scaly reptilian-like skin is, however, considered the plesiomorphic condition for theropods and dinosaurs more broadly. Here, we review the morphology and distribution of non-feathered integumentary structures in non-avialan theropods, covering squamous skin and naked skin as well as dermal ossifications. The integumentary record of non-averostran theropods is limited to tracks, which ubiquitously show a covering of tiny reticulate scales on the plantar surface of the pes. This is consistent also with younger averostran body fossils, which confirm an arthral arrangement of the digital pads. Among averostrans, squamous skin is confirmed in Ceratosauria (Carnotaurus), Allosauroidea (Allosaurus, Concavenator, Lourinhanosaurus), Compsognathidae (Juravenator), and Tyrannosauroidea (Santanaraptor, Albertosaurus, Daspletosaurus, Gorgosaurus, Tarbosaurus, Tyrannosaurus), whereas dermal ossifications consisting of sagittate and mosaic osteoderms are restricted to Ceratosaurus. Naked, non-scale bearing skin is found in the contentious tetanuran Sciurumimus, ornithomimosaurians (Ornithomimus) and possibly tyrannosauroids (Santanaraptor), and also on the patagia of scansoriopterygids (Ambopteryx, Yi). Scales are surprisingly conservative among non-avialan theropods compared to some dinosaurian groups (e.g. hadrosaurids); however, the limited preservation of tegument on most specimens hinders further interrogation. Scale patterns vary among and/or within body regions in Carnotaurus, Concavenator and Juravenator, and include polarised, snake-like ventral scales on the tail of the latter two genera. Unusual but more uniformly distributed patterning also occurs in Tyrannosaurus, whereas feature scales are present only in Albertosaurus and Carnotaurus. Few theropods currently show compelling evidence for the co-occurrence of scales and feathers (e.g. Juravenator, Sinornithosaurus), although reticulate scales were probably retained on the mani and pedes of many theropods with a heavy plumage. Feathers and filamentous structures appear to have replaced widespread scaly integuments in maniraptorans. Theropod skin, and that of dinosaurs more broadly, remains a virtually untapped area of study and the appropriation of commonly used techniques in other palaeontological fields to the study of skin holds great promise for future insights into the biology, taphonomy and relationships of these extinct animals.Fil: Hendrickx, Christophe Marie Fabian. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Bell, Phil R.. University Of New England; AustraliaFil: Pittman, Michael. Colegio Universitario de Londres; Reino UnidoFil: Milner, Andrew R. C.. No especifíca;Fil: Cuesta Fidalgo, Elena. Bayerische Staatssammlung für Paläontologie und Geologie; AlemaniaFil: O'Connor, Jingmai. No especifíca;Fil: Loewen, Mark. No especifíca;Fil: Currie, Philip J.. University of Alberta; CanadáFil: Mateus, Octávio. Universidade Nova de Lisboa; PortugalFil: Kaye, Thomas G.. Foundation For Scientific Advancement; Estados UnidosFil: Delcourt, Rafael. Universidade Estadual de Campinas; Brasi

A unique therapeutic approach to emesis and itch with a proanthocyanidin-rich genonutrient

<p>Abstract</p> <p>Background</p> <p>We examined the therapeutic potential of a proprietary <it>Croton palanostigma </it>extract (Zangrado^®) in the management of emesis and itch.</p> <p>Methods</p> <p>Emesis was induced in ferrets with morphine-6-glucuronide (0.05 mg/kg sc) in the presence of Zangrado (3 mg/kg, ip) and the cannabinoid receptor 1 antagonist, AM 251 (5 mg/kg, ip). Topical Zangrado (1%) was assessed for anti-pruretic actions in the 5-HT-induced scratching model in rats and evaluated in capsaicin-induced gastric hyperemia as measured by laser doppler flow. In the <it>Apc</it>^<it>Min</it>mouse model of precancerous adenomatosis polyposis, mice received Zangrado (100 μg/ml in drinking water) from the age of 6 – 16 weeks for effects on polyp number. In RAW 264.7 cells Zangrado was examined for effects on lipopolysaccharide-induced nitrite production.</p> <p>Results</p> <p>Zangrado was a highly effective anti-emetic, reducing morphine-induced vomiting and retching by 77%. These benefits were not associated with sedation or hypothermia and were not reversed by cannabinoid receptor antagonism. Itch responses were blocked in both the morphine and 5-HT models. Zangrado did not exacerbate the <it>Apc</it>^<it>Min</it>condition rather health was improved. Capsaicin-induced hyperemia was blocked by Zangrado, which also attenuated the production of nitric oxide by activated macrophages.</p> <p>Conclusion</p> <p>Zangrado is an effective anti-emetic and anti-itch therapy that is devoid of common side-effects, cannabinoid-independent and broadly suppresses sensory afferent nerve activation. This complementary medicine represents a promising new approach to the management of nausea, itch and irritable bowel syndrome.</p