Soluble mediator secretion by non-stimulated and stimulated synovial tissue explants (STEs) from normal and OA donors.

<p>Graphs demonstrate the absolute levels of all soluble mediators, which were significantly (p<0.01) different between <b>A.</b> non-stimulated (black bars) and IL-1α-stimulated (white bars) normal STEs and <b>B.</b> non-stimulated (grey bars) and IL-1α-stimulated (white bars) OA STEs. Data were obtained from pooled supernatants representing the average of 6 STEs per donor and the production in a time period of 7 days. Data are plotted on a log scale. Bars indicate mean concentration (pg/ml culture medium) ± SD.</p

Percentage GAG release from cartilage explants cultured alone or together with STEs under non-stimulated and IL-1α-stimulated conditions.

<p>%GAG release is indicated as mean (SD).</p><p>Δ STE+cartilage: corrected value for GAG release in the cartilage explant.</p

Secreted MMP activity (rfu/sec) by STE and cartilage alone and by the co-culture of STE and cartilage under non-stimulated and Il-1α-stimulated conditions.

<p>Secreted MMP activity is indicated as mean (SD).</p

Proteoglycan degradation of healthy cartilage explants cultured alone or together with synovial tissue explants (STEs).

<p>Proteoglycan degradation was expressed as the percentage of glycosaminoglycans (GAG) released into the medium during 7 days of culture of cartilage alone or co-cultured together with normal (<b>A, B</b>) or OA (<b>C, D</b>) STEs without (<b>A, C</b>) or with (<b>B, D</b>) IL-1α stimulation. Each line represents an individual donor and connects the % GAG release of cartilage alone (left) with the matching co-culture condition of cartilage together with STEs (right). There were no significant differences between cartilage cultured alone or co-cultured with normal or OA STEs.</p

Soluble mediator secretion by normal and OA synovial tissue explants (STEs) with or without IL-1α.

<p>Graphs demonstrate all soluble mediators which were significantly (p<0.01) different between <b>A.</b> normal (black bars) and OA (grey bars) STEs and <b>B.</b> normal and OA STEs under pro-inflammatory (IL-1α) conditions. Data were obtained from pooled supernatants representing the average of 6 STEs per donor and the production in a time period of 7 days. Data are plotted on a log scale. Bars indicate mean concentration (pg/ml culture medium) ± SD.</p