ИЗУЧЕНИЕ ВЛИЯНИЯ ГИБРИДНЫХ МЕДИКО-ПСИХОЛОГИЧЕСКИХ И ПЕДАГОГИЧЕСКИХ ТЕХНОЛОГИЙ НА ПСИХОСОМАТИЧЕСКОЕ СОСТОЯНИЕ ЛЮДЕЙ В АКАДЕМИЧЕСКОЙ СРЕДЕ

This paper analyzes the influence of color, visual images, symbols and other external factors on the attitude and health of a person in a university environment. The article examines the influence of color as a powerful factor capable of influencing the psychoemotional sphere of a person and the manifestation of psychosomatic diseases. The authors have developed new medical-psychological and pedagogical algorithms for the use of color, visual images and fine motor skills on the psychophysical state of a person.В данной работе анализируется влияние цвета, зрительных образов, символов и других внешних факторов на мироощущение и здоровье человека в университетской среде. В статье рассматривается влияние цвета как мощного фактора, способного влиять на психоэмоциональную сферу человека и проявление психосоматических заболеваний. Авторами разработаны новые медико-психологические и педагогические алгоритмы использования цвета, зрительных образов и мелкой моторики на психофизическое состояние человека

To the question of the relevance of the study of the effect of color on humans

This article attempts to explain the influence of color, visual images, and other external factors on people's perception and human health. The article analyzes the influence of color as a powerful physical factor that can affect stressful conditions. Algorithms have been developed for influencing the psychophysical state of a person in normal and pathological conditions

Increased suppressor activity of transformed ex vivo regulatory T-cells in comparison with unstimulated cells of the same donor [Povyshennaia supressornaia aktivnost' transformirovannykh ex vivo reguliatornykh T-kletok v sravnenii s nestimulirovannymi kletkami togo zhe donora]

Regulatory T-cells CD4⁺CD25⁺FoxP3⁺CD127low (Tregs) play a key role in the maintenance of tolerance to auto antigens, inhibit function of effector T and B lymphocytes, and provide a balance between effector and regulatory arms of immunity. Patients with autoimmune diseases have decreased Treg numbers and impaired suppressive activity. Transformed ex vivo autologous Tregs could restore destroyed balance of the immune system. We developed a method for Treg precursor cell cultivation. Following the method, we were able to grown up 300-400 million of Tregs cells from 50 ml of peripheral blood during a week. Transformed ex vivo Tregs are 90-95% CD4⁺CD25⁺FoxP3⁺CD127low and have increased expression of transcription genes FoxP3 and Helios. Transformed ex vivo Tregs have increased demethylation of FoxP3 promoter and activated genes of proliferation markers Cycline B1, Ki67 and LGALS 1. Transformed ex vivo Tregs have increased suppressive activity and up to 80-90% these cells secrete cytokines TNFα и IFNγ. Our data suggest transformed ex vivo autologous Tregs have genetic, immunophenotypic and functional characteristics for regulatory T-cells and further can be used for adoptive immunotherapy autoimmune diseases and inhibition of transplantation immunity.Reguliatornye T-kletki CD4⁺CD25⁺FoxP3⁺SD127low (Treg) igraiut kliuchevuiu rol' v podderzhanii tolerantnosti k autoantigenam, podavliaiut funktsiiu éffektornykh T- i V-limfotsitov i obespechivaiut balans mezhdu éffektornym i reguliatornym zvenom immuniteta. U bol'nykh s autoimmunnymi zabolevaniiami snizheno soderzhanie Treg i narushena funktsiia étikh kletok. Zamestitel'naia terapiia autologichnymi kletkami patsienta, vyrashchennymi ex vivo, mozhet vosstanovit' narushennyĭ balans immunnoĭ sistemy. Nami razrabotana metodika kul'tivirovaniia kletok-predshestvennikov Treg vne organizma cheloveka, kotoraia pozvoliaet iz 50 ml perifericheskoĭ krovi vyrastit' 300-400 mln Treg v techenie odnoĭ nedeli. V nastoiashchem issledovanii pokazano, chto po sravneniiu s Treg perifericheskoĭ krovi, vyrashchennye ex vivo kletki na 90-95% imeiut fenotip CD4⁺CD25⁺FoxP3⁺SD127low Treg i povyshennuiu ékspressiiu kliuchevykh genov transkriptsii FoxP3 i Helios. V transformirovannykh Treg povyshena stepen' demetilirovaniia v promotornom uchastke gena FoxP3 i aktivirovany geny-markery proliferatsii tsiklina V1, Ki67 i LGALS 1. Transformirovannye ex vivo Treg obladaiut povyshennoĭ supressornoĭ aktivnost'iu, i do 80-90% kletok v populiatsii sekretiruiut tsitokiny TNFα i IFNγ. Nashi dannye pokazyvaiut, chto vyrashchennye ex vivo autologichnye Treg kletki imeiut geneticheskie, markernye i funktsional'nye kharakteristiki reguliatornykh T-kletok, kotorye v budushchem mogut byt' ispol'zovany dlia adaptivnoĭ immunoterapii bol'nykh s autoimmunnymi zabolevaniiami, a takzhe dlia podavleniia transplantatsionnogo immuniteta, v chastnosti, reaktsii transplantata protiv khoziaina